Articles

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

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IssueVol 4 No 1 (2012) QRcode
SectionArticles
Keywords
Mycobacterium tuberculosis Pulsed-filed gel electrophoresis TB-complex
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How to Cite
1.
Ghodousi A, Vatani S, Darban-Sarokhalil D, Omrani M, Fooladi A, Khosaravi A, Feizabadi MM. Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex. Iran J Microbiol. 1;4(1):44-46.