Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 4 1 2012 03 15 44 46 EN Arash Ghodousi Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. S Vatani ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.. Davood Darban-Sarokhalil Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Maryam Omrani Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AA Fooladi Research Center of Molecular Biology, Department of Pathology, School of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran. Ad Khosaravi ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Mohammad Mehdi Feizabadi Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 16 A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria. https://ijm.tums.ac.ir/index.php/ijm/article/view/713 https://ijm.tums.ac.ir/index.php/ijm/article/download/713/480