Prevalence and capsular type distribution of group B Streptococcus isolated from vagina of pregnant women in Nghe An province, Vietnam
-
Tran Quang Hanh
,
Vu Van Du
,
Pham Thu Hien
,
Duong Dinh Chinh
,
Cao Ba Loi
,
Nguyen Manh Dung
,
Do Ngoc Anh
,
Tran Thi Kieu Anh
Abstract
Background and Objectives: Identification of GBS serotypes provides helpful information for appropriate the development of suitable vaccines; however, no reports from Vietnam have been published. This study has been performed to find the prevalence and serotypes of group B Streptococcus isolated from vagina of pregnant women in Nghe An province, Vietnam.
Materials and Methods: Vaginal swabs were collected from pregnant women at 35-37 weeks of gestation at the Nghe An Obstetrics and Pediatrics Hospital, Vietnam between May 2018 and July 2019. The swabs were cultured on 5% sheep blood agar for isolation of GBS. All isolates were identified using the Gram staining, CAMP test and specific PCR. GBS strains were serotyped using the multiplex PCR assays.
Results: The prevalence of vaginal GBS colonization was 9.20% of 750 participants. Among the isolates, serotypes III (39.13%) and V (31.89%) were the most frequent, followed by serotypes Ia (11.59%), VI (11.59%), Ib (2.90%), II (1.45%) and VII (1.45%), respectively. Serotypes IV, VIII and IX were not found.
Conclusion: The prevalence of GBS in the Nghe An province of central Vietnam was similar to reports from other parts of the world. The predominat GBS serotypes (III, V, Ia and VI) were slightly different from those previously described from other regions around the world. The high frequency of serotype VI was a notable feature of the strains from pregnant women in Vietnam.
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2. Poyart C, Tazi A, Réglier-Poupet H, Billoët A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol 2007; 45:1985-1988.
3. Wang P, Ma Z, Tong J, Zhao R, Shi W, Yu S, et al. Serotype distribution, antimicrobial resistance, and molecular characterization of invasive group B Streptococcus isolates recovered from Chinese neonates. Int J Infect Dis 2015;37:115-118.
4. Raabe VN, Shane AL. Group B Streptococcus (Streptococcus agalactiae). Microbiol Spectr 2019;7(2): 10.1128/microbiolspec.
5. Imperi M, Pataracchia M, Alfarone G, Baldassarri L, Orefici G, Creti R. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. J Microbiol Methods 2010; 80:212-214.
6. Mukesi M, Iweriebor BC, Obi LC, Nwodo UU, Moyo SR, Okoh AI. Prevalence and capsular type distribution of Streptococcus agalactiae isolated from pregnant women in Namibia and South Africa. BMC Infect Dis 2019; 19:179.
7. Morozumi M, Wajima T, Takata M, Iwata S, Ubukata K. Molecular characteristics of group B Streptococci isolated from adults with invasive infections in Japan. J Clin Microbiol 2016; 54:2695-2700.
8. Chen VL, Avci FY, Kasper DL. A maternal vaccine against group B Streptococcus: past, present, and future. Vaccine 2013;31:D13-D19.
9. Verani JR, McGee L, Schrag SJ; Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC). Prevention of perinatal group B streptococcal disease-revised guidelines from CDC, 2010. MMWR Recomm Rep 2010;59:1-36.
10. Dangor Z, Lala SG, Cutland CL, Koen A, Jose L, Nakwa F, et al. Burden of invasive group B Streptococcus disease and early neurological sequelae in South African infants. PLoS One 2015; 10(4):e0123014.
11. Skoff TH, Farley MM, Petit S, Craig AS, Schaffner W, Gershman K, et al. Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990-2007. Clin Infect Dis 2009; 49:85-92.
12. Ballard MS, Schonheyder HC, Knudsen JD, Lyytikainen O, Dryden M, Kennedy KJ, et al. The changing epidemiology of group B Streptococcus bloodstream infection: a multi-national population-based assessment. Infect Dis (Lond) 2016; 48:386-391.
13. Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL. Serotype IX, a proposed new Streptococcus agalactiae serotype. J Clin Microbiol 2007; 45:2929-2936.
14. Slotved HC, Hoffmann S. Evaluation of procedures for typing of group B Streptococcus: a retrospective study. PeerJ 2017;5:e3105.
15. Wang P, Tong J-j, Ma X-h, Song F-l, Fan L, Guo C-m, et al. Serotypes, antibiotic susceptibilities, and multi-locus sequence type profiles of Streptococcus agalactiae isolates circulating in Beijing, China. PLoS One 2015; 10(3):e0120035.
16. Lu B, Li D, Cui Y, Sui W, Huang L, Lu X. Epidemiology of Group B Streptococcus isolated from pregnant women in Beijing, China. Clin Microbiol Infect 2014; 20:O370-O373.
17. Jannati E, Roshani M, Arzanlou M, Habibzadeh S, Rahimi G, Shapuri R. Capsular serotype and antibiotic resistance of group B streptococci isolated from pregnant women in Ardabil, Iran. Iran J Microbiol 2012; 4:130-135.
18. Africa CWJ, Kaambo E. Group B Streptococcus Serotypes in Pregnant Women From the Western Cape Region of South Africa. Front Public Health 2018; 6:356.
19. Kim DH, Min BJ, Jung EJ, Byun JM, Jeong DH, Lee KB, et al. Prevalence of group B Streptococcus colonization in pregnant women in a tertiary care center in Korea. Obstet Gynecol Sci 2018; 61:575-583.
20. Villanueva-Uy ME, Wongsiridej P, Sangtawesin V, Chiu V, Tallo V, Nazaire-Bermal N, et al. The burden of invasive neonatal Group B streptococcal (GBS) disease in Thailand and the Philippines. Southeast Asian J Trop Med Public Health 2015; 46:728-737.
21. Miller CS, Handley KM, Wrighton KC, Frischkorn KR, Thomas BC, Banfield JF. Short-read assembly of full-length 16S amplicons reveals bacterial diversity in subsurface sediments. PLoS One 2013; 8(2):e56018.
22. Russell NJ, Seale AC, O'Driscoll M, O'Sullivan C, Bianchi-Jassir F, Gonzalez-Guarin J, et al. Maternal colonization with group B Streptococcus and serotype distribution worldwide: systematic review and meta-analyses. Clin Infect Dis 2017;65(suppl_2):S100-S111.
23. Stein RA. Group B streptococci in pregnancy: New perspectives for old challenges. Int J Clin Pract 2019;73(5):e13340.
24. Namavar Jahromi B, Poorarian S, Poorbarfehee S. The prevalence and adverse effects of group B streptococcal colonization during pregnancy. Arch Iran Med 2008;11:654-657.
25. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B Streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis 2010;10:336.
26. Hong JS, Choi CW, Park KU, Kim SN, Lee HJ, Lee HR, et al. Genital group B Streptococcus carrier rate and serotype distribution in Korean pregnant women: implications for group B streptococcal disease in Korean neonates. J Perinat Med 2010; 38:373-377.
27. Nkembe NM, Kamga HG, Baiye WA, Chafa AB, Njotang PN. Streptococcus agalactiae prevalence and antimicrobial susceptibility pattern in vaginal and anorectal swabs of pregnant women at a tertiary hospital in Cameroon. BMC Res Notes 2018; 11:480.
28. Sharmila V, Joseph NM, Arun Babu T, Chaturvedula L, Sistla S. Genital tract group B streptococcal colonization in pregnant women: a South Indian perspective. J Infect Dev Ctries 2011; 5:592-595.
29. Ali MM, Woldeamanuel Y, Woldetsadik DA, Chaka TE, Fenta DA, Dinberu MT, et al. Prevalence of group B Streptococcus among pregnant women and newborns at Hawassa University comprehensive specialized hospital, Hawassa, Ethiopia. BMC Infect Dis 2019; 19:325.
30. El-Kersh TA, Al-Nuaim LA, Kharfy TA, Al-Shammary FJ, Al-Saleh SS, Al-Zamel FA. Detection of genital colonization of group B streptococci during late pregnancy. Saudi Med J 2002; 23:56-61.
31. Wollheim C, Sperhacke RD, Fontana SKR, Vanni AC, Kato SK, Araujo PR, et al. Group B Streptococcus detection in pregnant women via culture and PCR methods. Rev Soc Bras Med Trop 2017; 50:179-183.
32. Rausch AV, Gross A, Droz S, Bodmer T, Surbek DV. Group B Streptococcus colonization in pregnancy: prevalence and prevention strategies of neonatal sepsis. J Perinat Med 2009; 37:124-129.
33. Ji W, Zhang L. Colonization prevalence and antibiotic susceptibility of Group B Streptococcus in pregnant women over a 6-year period in Dongguan, China. PLoS One 2017; 12(8):e0183083.
34. Song JY, Lim JH, Lim S, Yong Z, Seo HS. Progress toward a group B streptococcal vaccine. Hum Vaccin Immunother 2018;14:2669-2681.
35. Chien-Chung Lee, Jen-Fu Hsu, Rajendra Prasad Janapatla, Chyi-Liang Chen, Ying-Li Zhou, Reyin Lien, Cheng-Hsun. Clinical and microbiological characteristics of group B Streptococcus from pregnant women and diseased infants in intrapartum antibiotic prophylaxis Era in Taiwan. Sci Rep 2019; 9:13525.
36. Madrid L, Seale AC, Kohli-Lynch M, Edmond KM, Lawn JE, Heath PT, et al. Infant group B Streptococcal disease incidence and serotypes worldwide: systematic review and meta-analyses. Clin Infect Dis 2017; 65(suppl_2):S160-S172.
37. Lin SM, Zhi Y, Ahn KB, Lim S, Seo HS. Status of group B streptococcal vaccine development. Clin Exp Vaccine Res 2018; 7:76-81.
38. Karunakaran R, Raja NS, Hafeez A, Puthucheary SD. Group B Streptococcus infection: epidemiology, serotypes, and antimicrobial susceptibility of selected isolates in the population beyond infancy (excluding females with genital tract- and pregnancy-related isolates) at the University Malaya Medical Centre, Kuala Lumpur. Jpn J Infect Dis 2009; 62:192-194.
39. Dhanoa A, Karunakaran R, Puthucheary SD. Serotype distribution and antibiotic susceptibility of group B streptococci in pregnant women. Epidemiol Infect 2010;138:979-981.
40. Whitney CG, Daly S, Limpongsanurak S, Festin MR, Thinn KK, Chipato T, et al. The international infections in pregnancy study: group B streptococcal colonization in pregnant women. J Matern Fetal Neonatal Med 2004; 15:267-274.
41. Shabayek S, Abdalla S, Abouzeid AM. Serotype and surface protein gene distribution of colonizing group B Streptococcus in women in Egypt. Epidemiol Infect 2014; 142:208-210.
42. Slotved H-C, Dayie NTKD, Banini JAN, Frimodt-Møller N. Carriage and serotype distribution of Streptococcus agalactiae in third trimester pregnancy in southern Ghana. BMC Pregnancy Childbirth 2017; 17:238.
43. Botelho ACN, Oliveira JG, Damasco AP, Santos KTB, Ferreira AFM, Rocha GT, et al. Streptococcus agalactiae carriage among pregnant women living in Rio de Janeiro, Brazil, over a period of eight years. PLoS One 2018; 13(5):e0196925.
2. Poyart C, Tazi A, Réglier-Poupet H, Billoët A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol 2007; 45:1985-1988.
3. Wang P, Ma Z, Tong J, Zhao R, Shi W, Yu S, et al. Serotype distribution, antimicrobial resistance, and molecular characterization of invasive group B Streptococcus isolates recovered from Chinese neonates. Int J Infect Dis 2015;37:115-118.
4. Raabe VN, Shane AL. Group B Streptococcus (Streptococcus agalactiae). Microbiol Spectr 2019;7(2): 10.1128/microbiolspec.
5. Imperi M, Pataracchia M, Alfarone G, Baldassarri L, Orefici G, Creti R. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. J Microbiol Methods 2010; 80:212-214.
6. Mukesi M, Iweriebor BC, Obi LC, Nwodo UU, Moyo SR, Okoh AI. Prevalence and capsular type distribution of Streptococcus agalactiae isolated from pregnant women in Namibia and South Africa. BMC Infect Dis 2019; 19:179.
7. Morozumi M, Wajima T, Takata M, Iwata S, Ubukata K. Molecular characteristics of group B Streptococci isolated from adults with invasive infections in Japan. J Clin Microbiol 2016; 54:2695-2700.
8. Chen VL, Avci FY, Kasper DL. A maternal vaccine against group B Streptococcus: past, present, and future. Vaccine 2013;31:D13-D19.
9. Verani JR, McGee L, Schrag SJ; Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC). Prevention of perinatal group B streptococcal disease-revised guidelines from CDC, 2010. MMWR Recomm Rep 2010;59:1-36.
10. Dangor Z, Lala SG, Cutland CL, Koen A, Jose L, Nakwa F, et al. Burden of invasive group B Streptococcus disease and early neurological sequelae in South African infants. PLoS One 2015; 10(4):e0123014.
11. Skoff TH, Farley MM, Petit S, Craig AS, Schaffner W, Gershman K, et al. Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990-2007. Clin Infect Dis 2009; 49:85-92.
12. Ballard MS, Schonheyder HC, Knudsen JD, Lyytikainen O, Dryden M, Kennedy KJ, et al. The changing epidemiology of group B Streptococcus bloodstream infection: a multi-national population-based assessment. Infect Dis (Lond) 2016; 48:386-391.
13. Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL. Serotype IX, a proposed new Streptococcus agalactiae serotype. J Clin Microbiol 2007; 45:2929-2936.
14. Slotved HC, Hoffmann S. Evaluation of procedures for typing of group B Streptococcus: a retrospective study. PeerJ 2017;5:e3105.
15. Wang P, Tong J-j, Ma X-h, Song F-l, Fan L, Guo C-m, et al. Serotypes, antibiotic susceptibilities, and multi-locus sequence type profiles of Streptococcus agalactiae isolates circulating in Beijing, China. PLoS One 2015; 10(3):e0120035.
16. Lu B, Li D, Cui Y, Sui W, Huang L, Lu X. Epidemiology of Group B Streptococcus isolated from pregnant women in Beijing, China. Clin Microbiol Infect 2014; 20:O370-O373.
17. Jannati E, Roshani M, Arzanlou M, Habibzadeh S, Rahimi G, Shapuri R. Capsular serotype and antibiotic resistance of group B streptococci isolated from pregnant women in Ardabil, Iran. Iran J Microbiol 2012; 4:130-135.
18. Africa CWJ, Kaambo E. Group B Streptococcus Serotypes in Pregnant Women From the Western Cape Region of South Africa. Front Public Health 2018; 6:356.
19. Kim DH, Min BJ, Jung EJ, Byun JM, Jeong DH, Lee KB, et al. Prevalence of group B Streptococcus colonization in pregnant women in a tertiary care center in Korea. Obstet Gynecol Sci 2018; 61:575-583.
20. Villanueva-Uy ME, Wongsiridej P, Sangtawesin V, Chiu V, Tallo V, Nazaire-Bermal N, et al. The burden of invasive neonatal Group B streptococcal (GBS) disease in Thailand and the Philippines. Southeast Asian J Trop Med Public Health 2015; 46:728-737.
21. Miller CS, Handley KM, Wrighton KC, Frischkorn KR, Thomas BC, Banfield JF. Short-read assembly of full-length 16S amplicons reveals bacterial diversity in subsurface sediments. PLoS One 2013; 8(2):e56018.
22. Russell NJ, Seale AC, O'Driscoll M, O'Sullivan C, Bianchi-Jassir F, Gonzalez-Guarin J, et al. Maternal colonization with group B Streptococcus and serotype distribution worldwide: systematic review and meta-analyses. Clin Infect Dis 2017;65(suppl_2):S100-S111.
23. Stein RA. Group B streptococci in pregnancy: New perspectives for old challenges. Int J Clin Pract 2019;73(5):e13340.
24. Namavar Jahromi B, Poorarian S, Poorbarfehee S. The prevalence and adverse effects of group B streptococcal colonization during pregnancy. Arch Iran Med 2008;11:654-657.
25. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B Streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis 2010;10:336.
26. Hong JS, Choi CW, Park KU, Kim SN, Lee HJ, Lee HR, et al. Genital group B Streptococcus carrier rate and serotype distribution in Korean pregnant women: implications for group B streptococcal disease in Korean neonates. J Perinat Med 2010; 38:373-377.
27. Nkembe NM, Kamga HG, Baiye WA, Chafa AB, Njotang PN. Streptococcus agalactiae prevalence and antimicrobial susceptibility pattern in vaginal and anorectal swabs of pregnant women at a tertiary hospital in Cameroon. BMC Res Notes 2018; 11:480.
28. Sharmila V, Joseph NM, Arun Babu T, Chaturvedula L, Sistla S. Genital tract group B streptococcal colonization in pregnant women: a South Indian perspective. J Infect Dev Ctries 2011; 5:592-595.
29. Ali MM, Woldeamanuel Y, Woldetsadik DA, Chaka TE, Fenta DA, Dinberu MT, et al. Prevalence of group B Streptococcus among pregnant women and newborns at Hawassa University comprehensive specialized hospital, Hawassa, Ethiopia. BMC Infect Dis 2019; 19:325.
30. El-Kersh TA, Al-Nuaim LA, Kharfy TA, Al-Shammary FJ, Al-Saleh SS, Al-Zamel FA. Detection of genital colonization of group B streptococci during late pregnancy. Saudi Med J 2002; 23:56-61.
31. Wollheim C, Sperhacke RD, Fontana SKR, Vanni AC, Kato SK, Araujo PR, et al. Group B Streptococcus detection in pregnant women via culture and PCR methods. Rev Soc Bras Med Trop 2017; 50:179-183.
32. Rausch AV, Gross A, Droz S, Bodmer T, Surbek DV. Group B Streptococcus colonization in pregnancy: prevalence and prevention strategies of neonatal sepsis. J Perinat Med 2009; 37:124-129.
33. Ji W, Zhang L. Colonization prevalence and antibiotic susceptibility of Group B Streptococcus in pregnant women over a 6-year period in Dongguan, China. PLoS One 2017; 12(8):e0183083.
34. Song JY, Lim JH, Lim S, Yong Z, Seo HS. Progress toward a group B streptococcal vaccine. Hum Vaccin Immunother 2018;14:2669-2681.
35. Chien-Chung Lee, Jen-Fu Hsu, Rajendra Prasad Janapatla, Chyi-Liang Chen, Ying-Li Zhou, Reyin Lien, Cheng-Hsun. Clinical and microbiological characteristics of group B Streptococcus from pregnant women and diseased infants in intrapartum antibiotic prophylaxis Era in Taiwan. Sci Rep 2019; 9:13525.
36. Madrid L, Seale AC, Kohli-Lynch M, Edmond KM, Lawn JE, Heath PT, et al. Infant group B Streptococcal disease incidence and serotypes worldwide: systematic review and meta-analyses. Clin Infect Dis 2017; 65(suppl_2):S160-S172.
37. Lin SM, Zhi Y, Ahn KB, Lim S, Seo HS. Status of group B streptococcal vaccine development. Clin Exp Vaccine Res 2018; 7:76-81.
38. Karunakaran R, Raja NS, Hafeez A, Puthucheary SD. Group B Streptococcus infection: epidemiology, serotypes, and antimicrobial susceptibility of selected isolates in the population beyond infancy (excluding females with genital tract- and pregnancy-related isolates) at the University Malaya Medical Centre, Kuala Lumpur. Jpn J Infect Dis 2009; 62:192-194.
39. Dhanoa A, Karunakaran R, Puthucheary SD. Serotype distribution and antibiotic susceptibility of group B streptococci in pregnant women. Epidemiol Infect 2010;138:979-981.
40. Whitney CG, Daly S, Limpongsanurak S, Festin MR, Thinn KK, Chipato T, et al. The international infections in pregnancy study: group B streptococcal colonization in pregnant women. J Matern Fetal Neonatal Med 2004; 15:267-274.
41. Shabayek S, Abdalla S, Abouzeid AM. Serotype and surface protein gene distribution of colonizing group B Streptococcus in women in Egypt. Epidemiol Infect 2014; 142:208-210.
42. Slotved H-C, Dayie NTKD, Banini JAN, Frimodt-Møller N. Carriage and serotype distribution of Streptococcus agalactiae in third trimester pregnancy in southern Ghana. BMC Pregnancy Childbirth 2017; 17:238.
43. Botelho ACN, Oliveira JG, Damasco AP, Santos KTB, Ferreira AFM, Rocha GT, et al. Streptococcus agalactiae carriage among pregnant women living in Rio de Janeiro, Brazil, over a period of eight years. PLoS One 2018; 13(5):e0196925.
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i1.2511 | |
| Keywords | ||
| Group B Streptococcus Prevalence Serotypes Nghe An Vietnam | ||
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How to Cite
1.
Hanh T, Du V, Hien P, Chinh D, Loi C, Dung N, Anh D, Anh TT. Prevalence and capsular type distribution of group B Streptococcus isolated from vagina of pregnant women in Nghe An province, Vietnam. Iran J Microbiol. 2020;12(1):11-17.
