Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 12 1 2020 03 02 11 17 Tran Hanh Department of Obstetrics, Nghe An Obstetrics and Pediatrics Hospital, Nghe An, Vietnam Vu Du Department of Treatment on Demand, National Hospital of Obstetrics and Gynecology, Hanoi, Vietnam Pham Hien Department of Treatment on Demand, National Hospital of Pediatrics, Hanoi, Vietnam Duong Chinh Department of Neurology, Nghe An Friendship General Hospital, Nghe An, Vietnam Cao Loi Department of Science and Training, National Institute of Malaria Parasitology and Entomology, Hanoi, Vietnam Nguyen Dung Department of Anesthesiology and Critical Care, 108 Military Centre Hospital, Hanoi, Vietnam Do Anh Department of Medical Parasitology, Military Medical University, Hanoi, Vietnam Tran Thi Anh Department of Paediatric, Vinh Medical University, Nghe An, Vietnam 2019 10 14 2019 12 22 Background and Objectives: Identification of GBS serotypes provides helpful information for appropriate the development of suitable vaccines; however, no reports from Vietnam have been published. This study has been performed to find the prevalence and serotypes of group B Streptococcus isolated from vagina of pregnant women in Nghe An province, Vietnam. Materials and Methods: Vaginal swabs were collected from pregnant women at 35-37 weeks of gestation at the Nghe An Obstetrics and Pediatrics Hospital, Vietnam between May 2018 and July 2019. The swabs were cultured on 5% sheep blood agar for isolation of GBS. All isolates were identified using the Gram staining, CAMP test and specific PCR. GBS strains were serotyped using the multiplex PCR assays. Results: The prevalence of vaginal GBS colonization was 9.20% of 750 participants. Among the isolates, serotypes III (39.13%) and V (31.89%) were the most frequent, followed by serotypes Ia (11.59%), VI (11.59%), Ib (2.90%), II (1.45%) and VII (1.45%), respectively. Serotypes IV, VIII and IX were not found. Conclusion: The prevalence of GBS in the Nghe An province of central Vietnam was similar to reports from other parts of the world. The predominat GBS serotypes (III, V, Ia and VI) were slightly different from those previously described from other regions around the world. The high frequency of serotype VI was a notable feature of the strains from pregnant women in Vietnam. https://ijm.tums.ac.ir/index.php/ijm/article/view/2363 https://ijm.tums.ac.ir/index.php/ijm/article/download/2363/1216