Original Article

A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain


Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis.
Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.
Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay.
Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.

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IssueVol 12 No 1 (2020) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijm.v12i1.2516
Bordetella pertussis Extraction Outer membrane vesicle Vaccine

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How to Cite
Sekhavati M, Mohabati Mobarez A, Siadat SD, Noofeli M. A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain. Iran J Microbiol. 2020;12(1):37-42.