A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain
,
Abstract
Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis.
Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.
Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay.
Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.
1. Dou M, Sanchez J, Tavakoli H, Gonzalez JE, Sun J, Bard JD, et al. A low-cost microfluidic platform for rapid and instrument-free detection of whooping cough. Anal Chim Acta 2019;1065:71-78.
2. Ross PJ, Sutton CE, Higgins S, Allen AC, Walsh K, Misiak A, et al. Relative contribution of Th1 and Th17 cells in adaptive immunity to Bordetella pertussis: towards the rational design of an improved acellular pertussis vaccine. PLoS Pathog 2013;9(4):e1003264.
3. Hendriksz T, Pierce-Talsma S. Osteopathic manipulative medicine use in pediatric patients with pertussis. J Am Osteopath Assoc 2019;119(2):e9-e10.
4. Chiappini E, Stival A, Galli L, De Martino M. Pertussis re-emergence in the post-vaccination era. BMC Infect Dis 2013;13:151.
5. Trainor EA, Nicholson TL, Merkel TJ. Bordetella pertussis transmission. Pathog Dis 2015;73(8):ftv068.
6. Wendelboe AM, Njamkepo E, Bourillon A, Floret DD, Gaudelus J, Gerber M, et al. Transmission of Bordetella pertussis to young infants. Pediatr Infect Dis J 2007;26:293-299.
7. Skoff TH, Kenyon C, Cocoros N, Liko J, Miller L, Kudish K, et al. Sources of infant pertussis infection in the United States. Pediatrics 2015;136:635-641.
8. Jefferson T, Rudin M, DiPietrantonj C. Systematic review of the effects of pertussis vaccines in children. Vaccine 2003;21:2003-2014.
9. Amirthalingam G, Gupta S, Campbell H. Pertussis immunisation and control in England and Wales, 1957 to 2012: a historical review. Euro Surveill 2013;18: 20587.
10. Yeung KHT, Duclos P, Nelson EAS, Hutubessy RCW. An update of the global burden of pertussis in children younger than 5 years: a modelling study. Lancet Infect Dis 2017;17:974-980.
11. Burdin N, Handy LK, Plotkin SA. What is wrong with pertussis vaccine immunity? The problem of waning effectiveness of pertussis vaccines. Cold Spring Harb Perspect Biol 2017;9(12): a029454.
12. Sato Y, Sato H. Development of acellular pertussis vaccines. Biologicals 1999; 27: 61-69.
13. Cherry JD. Why do pertussis vaccines fail? Pediatrics 2012; 129:968-970.
14. Clark TA. Changing pertussis epidemiology: everything old is new again. J Infect Dis 2014; 209:978-981.
15. Gasperini G, Biagini M, Arato V, Gianfaldoni C, Vadi A, Norais N, et al. Outer membrane vesicles (OMV)-based and proteomics-driven antigen selection identifies novel factors contributing to Bordetella pertussis adhesion to epithelial cells. Mol Cell Proteomics 2018;17:205-215.
16. Gaillard ME, Bottero D, Moreno G, Rumbo M, Hozbor D. Strategies and new developments to control pertussis, an actual health problem. Pathog Dis 2015; 73(8): ftv059.
17. Diavatopoulos DA, Edwards KM. What is wrong with pertussis vaccine immunity? Why immunological memory to pertussis is failing. Cold Spring Harb Perspect Biol 2017;9(12): a029553.
18. Kulp A, Kuehn MJ. Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu Rev Microbiol 2010;64:163-184.
19. Lee EY, Choi DS, Kim KP, Gho YS. Proteomics in gram-negative bacterial outer membrane vesicles. Mass Spectrom Rev 2008;27:535-555.
20. Roier S, Zingl FG, Cakar F, Durakovic S, Kohl P, Eichmann TO, et al. A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria. Nat Commun 2016;7:10515.
21. Asensio CJA, Gaillard ME, Moreno G, Bottero D, Zurita E, Rumbo M, et al. Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate. Vaccine 2011; 29:1649-1656.
22. Gaillard ME, Bottero D, Errea A, Ormazábal M, Zurita ME, Moreno G, et al. Acellular pertussis vaccine based on outer membrane vesicles capable of conferring both long-lasting immunity and protection against different strain genotypes. Vaccine 2014; 32:931-937.
23. Stainer D, Scholte M. A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol 1970; 63:211-220.
24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-254.
25. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
26. Roberts R, Moreno G, Bottero D, Gaillard ME, Fingermann M, Graieb A, et al. Outer membrane vesicles as acellular vaccine against pertussis. Vaccine 2008;26: 4639-4646.
27. Hozbor D, Rodriguez M, Fernandez J, Lagares A, Guiso N, Yantorno O. Release of outer membrane vesicles from Bordetella pertussis. Curr Microbiol 1999; 38: 273-278.
28. Harlowe E, Lane D. Using Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1999.
29. Zurita ME, Wilk MW, Carriquiriborde F, Bartel E, Moreno GN, Misiak A, et al. A pertussis outer membrane vesicle-based vaccine induces lung-resident memory CD4 T cells and protection against Bordetella pertussis, including pertactin deficient strains. Front Cell Infect Microbiol 2019;9:125.
2. Ross PJ, Sutton CE, Higgins S, Allen AC, Walsh K, Misiak A, et al. Relative contribution of Th1 and Th17 cells in adaptive immunity to Bordetella pertussis: towards the rational design of an improved acellular pertussis vaccine. PLoS Pathog 2013;9(4):e1003264.
3. Hendriksz T, Pierce-Talsma S. Osteopathic manipulative medicine use in pediatric patients with pertussis. J Am Osteopath Assoc 2019;119(2):e9-e10.
4. Chiappini E, Stival A, Galli L, De Martino M. Pertussis re-emergence in the post-vaccination era. BMC Infect Dis 2013;13:151.
5. Trainor EA, Nicholson TL, Merkel TJ. Bordetella pertussis transmission. Pathog Dis 2015;73(8):ftv068.
6. Wendelboe AM, Njamkepo E, Bourillon A, Floret DD, Gaudelus J, Gerber M, et al. Transmission of Bordetella pertussis to young infants. Pediatr Infect Dis J 2007;26:293-299.
7. Skoff TH, Kenyon C, Cocoros N, Liko J, Miller L, Kudish K, et al. Sources of infant pertussis infection in the United States. Pediatrics 2015;136:635-641.
8. Jefferson T, Rudin M, DiPietrantonj C. Systematic review of the effects of pertussis vaccines in children. Vaccine 2003;21:2003-2014.
9. Amirthalingam G, Gupta S, Campbell H. Pertussis immunisation and control in England and Wales, 1957 to 2012: a historical review. Euro Surveill 2013;18: 20587.
10. Yeung KHT, Duclos P, Nelson EAS, Hutubessy RCW. An update of the global burden of pertussis in children younger than 5 years: a modelling study. Lancet Infect Dis 2017;17:974-980.
11. Burdin N, Handy LK, Plotkin SA. What is wrong with pertussis vaccine immunity? The problem of waning effectiveness of pertussis vaccines. Cold Spring Harb Perspect Biol 2017;9(12): a029454.
12. Sato Y, Sato H. Development of acellular pertussis vaccines. Biologicals 1999; 27: 61-69.
13. Cherry JD. Why do pertussis vaccines fail? Pediatrics 2012; 129:968-970.
14. Clark TA. Changing pertussis epidemiology: everything old is new again. J Infect Dis 2014; 209:978-981.
15. Gasperini G, Biagini M, Arato V, Gianfaldoni C, Vadi A, Norais N, et al. Outer membrane vesicles (OMV)-based and proteomics-driven antigen selection identifies novel factors contributing to Bordetella pertussis adhesion to epithelial cells. Mol Cell Proteomics 2018;17:205-215.
16. Gaillard ME, Bottero D, Moreno G, Rumbo M, Hozbor D. Strategies and new developments to control pertussis, an actual health problem. Pathog Dis 2015; 73(8): ftv059.
17. Diavatopoulos DA, Edwards KM. What is wrong with pertussis vaccine immunity? Why immunological memory to pertussis is failing. Cold Spring Harb Perspect Biol 2017;9(12): a029553.
18. Kulp A, Kuehn MJ. Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu Rev Microbiol 2010;64:163-184.
19. Lee EY, Choi DS, Kim KP, Gho YS. Proteomics in gram-negative bacterial outer membrane vesicles. Mass Spectrom Rev 2008;27:535-555.
20. Roier S, Zingl FG, Cakar F, Durakovic S, Kohl P, Eichmann TO, et al. A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria. Nat Commun 2016;7:10515.
21. Asensio CJA, Gaillard ME, Moreno G, Bottero D, Zurita E, Rumbo M, et al. Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate. Vaccine 2011; 29:1649-1656.
22. Gaillard ME, Bottero D, Errea A, Ormazábal M, Zurita ME, Moreno G, et al. Acellular pertussis vaccine based on outer membrane vesicles capable of conferring both long-lasting immunity and protection against different strain genotypes. Vaccine 2014; 32:931-937.
23. Stainer D, Scholte M. A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol 1970; 63:211-220.
24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-254.
25. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
26. Roberts R, Moreno G, Bottero D, Gaillard ME, Fingermann M, Graieb A, et al. Outer membrane vesicles as acellular vaccine against pertussis. Vaccine 2008;26: 4639-4646.
27. Hozbor D, Rodriguez M, Fernandez J, Lagares A, Guiso N, Yantorno O. Release of outer membrane vesicles from Bordetella pertussis. Curr Microbiol 1999; 38: 273-278.
28. Harlowe E, Lane D. Using Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1999.
29. Zurita ME, Wilk MW, Carriquiriborde F, Bartel E, Moreno GN, Misiak A, et al. A pertussis outer membrane vesicle-based vaccine induces lung-resident memory CD4 T cells and protection against Bordetella pertussis, including pertactin deficient strains. Front Cell Infect Microbiol 2019;9:125.
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i1.2516 | |
| Keywords | ||
| Bordetella pertussis Extraction Outer membrane vesicle Vaccine | ||
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How to Cite
1.
Sekhavati M, Mohabati Mobarez A, Siadat SD, Noofeli M. A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain. Iran J Microbiol. 2020;12(1):37-42.
