“In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
-
Mohammad Sekhavati
,
Keyvan Tadayon
,
Rainak Ghaderi
,
Reza Banihashemi
,
Ahmad Reza Jabbari
,
Gholamreza Shokri
,
Nasim Karimnasab
Abstract
Background and Objectives: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings.
Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
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| Files | ||
| Issue | Vol 7 No 1 (2015) | |
| Section | Original Article(s) | |
| Keywords | ||
| DNA size marker PCR sequencing Bacillus anthracis Sterne 34F2 | ||
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