Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 7 1 2015 10 13 45 49 EN Mohammad Sekhavati Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Keyvan Tadayon Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Rainak Ghaderi Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Reza Banihashemi Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Ahmad Reza Jabbari Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Gholamreza Shokri Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Nasim Karimnasab Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. 2015 10 12 2015 10 12 Background and Objectives: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker. Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding. https://ijm.tums.ac.ir/index.php/ijm/article/view/141 https://ijm.tums.ac.ir/index.php/ijm/article/download/141/123