Comparative effects of granulocyte-colony stimulating factor and colistin-alone or in combination on burn wound healing in Acinetobacter baumannii infected mice

  • Somayeh Soleymanzadeh Moghadam Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Zeinab Fagheei Aghmiyuni Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Hassan Zaheri Faculty of Veterinary Medicine, Islamic Azad University, Garmsar branch, Garmsar, Iran
  • Nastaran Arianpour Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Danaeifard Faculty of Veterinary Medicine, Islamic Azad University, Garmsar branch, Garmsar, Iran
  • Maryam Roham Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran http://orcid.org/0000-0001-5406-709X
  • Mahnoush Momeni Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
Keywords: Burn, Granulocyte-colony stimulating factor, Colistin, Acinetobacter baumannii, Wound infection

Abstract

Background and Objectives: Burn wounds are one of the most important health problems all over the world because infection after burn can delay wound healing. Treating burn wounds with granulocyte-colony stimulating factor (G-CSF) is known to improve healing of injured tissue. In addition, colistin is prescribed as an effective treatment. The aim of this study was to evaluate the effect of G-CSF and colistin alone or in combination with G-CSF on wound healing of Acinetobacter baumannii (A. baumannii) infected burns.Materials and Methods: This study was performed between January 2016 and April 2018. Burn wounds were experimentally induced in 36 mice. The wounds were inoculated with A. baumannii. In a 7-day period, burn wounds in each group were daily treated with subcutaneous injections (0.1 ml) of saline, G-CSF, colistin, and G-CSF plus colistin. After killing the animals, the size of the wound, number of leukocytes in the skin and microbial growth were evaluated. A value of p ≤ 0.05 was considered statistically significant. Results: Wound healing in the G-CSF plus colistin group was significantly higher than the control group and the G-CSF group (P = 0.023 and P = 0.033, respectively). In G-CSF+colistin group, the number of leukocytes was higher than the control group considerably (P = 0.007). On the 7th day of treatment, number of positive bacterial cultures in the colistin and the G-CSF plus colistin groups was lower than other groups with a significant difference.Conclusion: Concurrent consumption of G-CSF and antibiotics can control burn infection and enhance the immune system towards wound healing.

References

1. Mahmood Saffari FF, Pourbabaee M, Zibaei M. Evaluation of metallo-β-lactamase-production and carriage of bla-vim genes in Pseudomonas aeruginosa isolated from burn wound infections in Isfahan. Arch Trauma Res 2016; 5(4): e34343.
2. Leseva M, Arguirova M, Nashev D, Zamfirova E, Hadzhyiski O. Nosocomial infections in burn patients: etiology, antimicrobial resistance, means to control. Ann Burns Fire Disasters 2013;26:5-11.
3. Schwacha MG, Ayala A, Chaudry IH. Insights into the role of gammadelta T lymphocytes in the immunopathogenic response to thermal injury. J Leukoc Biol 2000;67:644-650.
4. Almasaudi SB. Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features. Saudi J Biol Sci 2018;25:586-596.
5. Goel N, Wattal C, Oberoi JK, Raveendran R, Datta S, Prasad KJ. Trend analysis of antimicrobial consumption and development of resistance in non-fermenters in a tertiary care hospital in Delhi, India. J Antimicrob Chemother 2011;66:1625-1630.
6. Aubin G, Portillo M, Trampuz A, Corvec S. Propionibacterium acnes, an emerging pathogen: from acne to implant-infections, from phylotype to resistance. Med Mal Infect 2014;44:241-250.
7. Bissell MJ, Radisky D. Putting tumours in context. Nat Rev Cancer 2001;1:46-54.
8. Babcock GF. Predictive medicine: severe trauma and burns. Cytometry B Clin Cytom 2003;53:48-53.
9. Layman H, Sacasa M, Murphy AE, Murphy AM, Pham SM, Andreopoulos FM. Co-delivery of FGF-2 and G-CSF from gelatin-based hydrogels as angiogenic therapy in a murine critical limb ischemic model. Acta Biomater 2009;5:230-239.
10. Fitzhugh CD, Hsieh MM, Bolan CD, Saenz C, Tisdale JF. Granulocyte colony-stimulating factor (G-CSF) administration in individuals with sickle cell disease: time for a moratorium? Cytotherapy 2009;11:464-471.
11. Fine J-D, Manes B, Frangoul H. Systemic granulocyte colony-stimulating factor (G-CSF) enhances wound healing in dystrophic epidermolysis bullosa (DEB): Results of a pilot trial. J Am Acad Dermatol 2015;73:56-61.
12. Huang H, Zhang Q, Liu J, Hao H, Jiang C, Han W. Granulocyte-colony stimulating factor (G-CSF) accelerates wound healing in hemorrhagic shock rats by enhancing angiogenesis and attenuating apoptosis. Med Sci Monit 2017;23:2644-2653.
13. Lee J, Han S, Jeon S, Hong T, Song W, Woo H, et al. Population pharmacokinetic analysis of colistin in burn patients. Antimicrob Agents Chemother 2013;57(5):2141-2146.
14. Hsueh P-R, Ko W-C, Wu J-J, Lu J-J, Wang F-D, Wu H-Y, et al. Consensus statement on the adherence to clinical and laboratory standards institute (CLSI) antimicrobial susceptibility testing guidelines (CLSI-2010 and CLSI-2010-update) for Enterobacteriaceae in clinical microbiology laboratories in Taiwan. J Microbiol Immunol Infect 2010;43:452-455.
15. Demers G, Griffin G, De Vroey G, Haywood JR, Zurlo J, Bédard M. Harmonization of animal care and use guidance. Science 2006;312:700-701.
16. Nishio N, Okawa Y, Sakurai H, Isobe K-i. Neutrophil depletion delays wound repair in aged mice. Age (Dordr) 2008;30:11-19.
17. Demling RH. Nutrition, anabolism, and the wound healing process: an overview. Eplasty 2009;9:e9.
18. Huseini HF, Rahimzadeh G, Fazeli MR, Mehrazma M, Salehi M. Evaluation of wound healing activities of kefir products. Burns 2012;38:719-723.
19. Black DS, Slavich GM. Mindfulness meditation and the immune system: a systematic review of randomized controlled trials. Ann N Y Acad Sci 2016;1373:13-24.
20. Jebur MS. Therapeutic efficacy of Lactobacillus acidophilus against bacterial isolates from burn wounds. N Am J Med Sci 2010;2:586-591.
21. Aksungar FB, Topkaya AE, Akyildiz M. Interleukin-6, C-reactive protein and biochemical parameters during prolonged intermittent fasting. Ann Nutr Metab 2007;51:88-95.
22. Abbas AK, Janeway CA. Immunology: improving on nature in the twenty-first century. Cell 2000;100:129-138.
23. Brubaker AL, Kovacs EJ. G-CSF enhances resolution of Staphylococcus aureus wound infection in an age-dependent manner. Shock 2013;40:327-333.
24. Harrison C. Innate immunity as a key element in host defense against methicillin resistant Staphylococcus aureus. Minerva Pediatr 2009;61:503-514.
25. Fernández-Reyes M, Rodríguez-Falcón M, Chiva C, Pachón J, Andreu D, Rivas L. The cost of resistance to colistin in Acinetobacter baumannii: a proteomic perspective. Proteomics 2009;9:1632-1645.
26. Barthelmes J, de Bazo AM, Pewzner-Jung Y, Schmitz K, Mayer CA, Foerch C, et al. Lack of ceramide synthase 2 suppresses the development of experimental autoimmune encephalomyelitis by impairing the migratory capacity of neutrophils. Brain Behav Immun 2015;46:280-292.
27. Goldring MB. The role of cytokines as inflammatory mediators in osteoarthritis: lessons from animal models. Connect Tissue Res 1999;40:1-11.
28. Cerci C, Ergin C, Eroglu E, Agalar C, Agalar F, Cerci S, et al. Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats. World J Gastroenterol 2007;13:2596-2599.
29. Jones A, Elphick H, Pettitt E, Everard M, Evans G. Colistin stimulates the activity of neutrophil elastase and Pseudomonas aeruginosa elastase. Eur Respir J 2002;19:1136-1141.
30. Hector A, Kappler M, Griese M. In vitro inhibition of neutrophil elastase activity by inhaled anti-Pseudomonas antibiotics used in cystic fibrosis patients. Mediators Inflamm 2010;2010:809591.
31. Salaheen S, Kim SW, Haley BJ, Van Kessel JAS, Biswas D. Alternative growth promoters modulate broiler gut microbiome and enhance body weight gain. Front Microbiol 2017;8:2088.
32. Khadem A, Soler L, Everaert N, Niewold TA. Growth promotion in broilers by both oxytetracycline and Macleaya cordata extract is based on their anti-inflammatory properties. Br J Nutr 2014;112:1110-1118.
33. MacGowan AP. Clinical implications of antimicrobial resistance for therapy. J Antimicrob Chemother 2008;62:ii105-ii14.
Published
2019-02-10
How to Cite
1.
Soleymanzadeh Moghadam S, Fagheei Aghmiyuni Z, Zaheri H, Arianpour N, Danaeifard MR, Roham M, Momeni M. Comparative effects of granulocyte-colony stimulating factor and colistin-alone or in combination on burn wound healing in Acinetobacter baumannii infected mice. Iran J Microbiol. 10(6):371-7.
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Original Article(s)