Comparison of antibacterial effects of a carrier produced in microemulsion system from aqueous extract of Aloe vera with selected antibiotics on Enterobacteriacea

  • Ghasem Habibi Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Mohammad Arjomandzadegan Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Maryam Tayeboon Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Farshideh Didgar Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Hossein Sarmadian Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Maryam Sadrnia Department of Biology, Payame Noor University, Tehran, Iran
  • Farid Mirhosseini Department of Chemistry, Faculty of Sciences, Arak University, Arak, Iran
  • Somayeh Geravand Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
  • Mahboobeh Abdoli Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
Keywords: Aloe vera, Extract, Antibacterial, Enterobacteriacea

Abstract

Background and Objectives: Antibiotics resistance has recently increased. The aim of this study was the evaluation of antibacterial efficacy of Aloe vera carrier produced in microemulsion system in comparison with ordinary antibiotics against some Enterobacteriacea. Materials and Methods: The aquatic extract of Aleo vera was produced by the Soxhlet method and a nonocarrier in the microemulsion system was prepared by two emulsifiers. The clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Shigella dysenteriae, Salmonella Typhimurium, Salmonella Paratyphi, Serratia marcescens, Proteus mirabilis, Enterobacter aerogenes, Citrobacter freundii and Morganella morganii were obtained from patients and were identified by microbiological methods. Diffusion disk was used for evaluation of antibacterial properties in comparison with selected ordinary antibiotics. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for tested materials were determined using MTT in the Micro Broth dilution method. Results: The results proved that effect of carrier on studied isolates is dependent on concentration level. The inhibitory effect of carrier in concentration of 15 µg/ml by 18 mm zone of inhibition for Klebsiella pneumoniae was comparable to Ceftazidime and Cefalothin. The lowest MIC and MBC determined by the Microbroth dilution method with MTT belonged to Klebsiella pneumoniae as 0.1 and 3 µg/ml and higher concentrations belonged to Enterobacter aerogenes at 7 and 15 µg/ml. The greatest effect of carrier of Aleo vera aquatic extract was observed for Klebsiella pneumoniae and the lowest effect belonged to Enterobacter aerogenes, Citrobacter freundii and Morganella morganii. Conclusion: It was concluded that the carrier of Aloe vera produced in microemulsion system was most effective and had equal effects in comparison with ordinary antibiotics against Enterobacteriacea.

References

Berahou A1, Auhmani A, Fdil N, Benharref A, Jana M, Gadhi CA., Antibacterial activity of Quercus ilex bark's extracts. J Ethnopharmacol. 2007 Jul 25;112(3):426-9. Epub 2007 Apr 5.

Surjushe A1, Vasani R, Saple DG., Aloe vera: a short review. Indian J Dermatol. 2008;53(4):163-6. doi: 10.4103/0019-5154.44785.

Arjomandzadegan M, Emami N, Habibi G, Farazi AA, Kahbazi M, Sarmadian H, Jabbari M, Hosseini H, Ramezani M. ,Antimycobacterial activity assessment of three ethnobotanical plants against Mycobacterium Tuberculosis: An In Vitro study, Int J Mycobacteriol. 2016 Dec;5 Suppl 1:S108-S109. doi: 10.1016/j.ijmyco.2016.10.025.

Kargaran M ; Ali Reza Moradabadi ; Mohammad Arjomandzadegan ; Hossein Hosseini ; Ghasem Habibi ; Maryam Tayeboon ; Hadi Karami ; and Ahmad Akbari, Effects of the Aqueous Extract of Aloe vera on the Morphological and Physiological Properties of E. coli, Iranian Red Crescent Medical Journal. 19(2): e23896 , DOI: 10.5812/ircmj.23896

Sadrnia M, Arjomandzadegan M. Comparative Study on the Effects of Aloe Vera Extract in Clinical Strains of Staphylococcus Aureus, Klebsiella, Staphylococcus Epidermidis and Escherichia Coli Compared to Antibiotics of Choice. Arak Medical University Journal. 2014; 17 (6) :39-46

Kahbazi M1, Dorreh F, Najmi AR, Arjomandzadegan M., Aetiology of dysentery in infants in infection ward of Amir Kabir Hospital, Arak, in 2010-2011.J Ayub Med Coll Abbottabad. 2011 Jul-Sep;23(3):91-3.

Kahbazi M, Ebrahimi M, Zarinfar N, Arjomandzadegan M, Efficacy of Synbiotics for Treatment of Bacillary Dysentery in Children: A Double-Blind, Randomized, Placebo-Controlled Study. Adv Med. 2016;2016:3194010. doi: 10.1155/2016/3194010.

Ramachandra CT, Srinivasa Rao P (2008) Processing of Aloe vera Leaf Gel: A Review. American Journal of Agricultural and Biological Sciences 3 (2): 502-510.

Ni Y, Tizard IR (2004) Analytical methodology: the gel-analysis of aloe pulp and its derivatives. In: Reynolds T (eds), Aloes The Genus Aloe. CRC Press, Boca Raton, Florida.

Ni Y, Turner D, Yates KM, Tizard I (2004) Isolation and characterization of structural components of Aloe vera L. leaf pulp. Int Immunopharmacol 4: 1745-1755.

Eshun K, He Q (2004) Aloe vera: A valuable ingredient for the food, pharmaceutical and cosmetic industries – A review. Crit Rev Food Sci Nutr 44: 91-96.

Boudreau MD, Beland FA (2006) An evaluation of the biological and toxicological properties of Aloe Barbadensis (Miller), Aloe vera. J Environ Sci Health C 24: 103-154.

Cosmetic Ingredient Review Expert Panel (2007). Final report on the safety assessment of Aloe andongensis extract, Aloe andongensis leaf juice, Aloe arborescens leaf extract, Aloe arborescens leaf juice, Aloe arborescens leaf protoplasts, Aloe barbadensis flower extract, Aloe barbadensis leaf, Aloe barbadensis leaf extract, Aloe barbadensis leaf juice, Aloe barbadensis leaf polysaccharides, Aloe barbadensis leaf water, Aloe ferox leaf extract, Aloe ferox leaf juice and Aloe ferox leaf juice extract. Int J Toxicol 26: 1-50.

Setareh, M., Javaherdashti, R., Assessment and control of MIC in a sugar cane factory, Materials and Corrosion, 2003, 54(4), pp. 259-263

Jawetz, Melnick, & Adelberg's medical microbiology, George F Brooks; Ernest Jawetz; Joseph L Melnick; Edward A Adelberg New York : McGraw Hill Medical, ©2010, 25th ed

Parker MT. Hospital acquired infections: Guidelines to laboratory methods. Copenhagen: WHO Regional Publication European; 1978: 35.

Gupta P, Murali P, Murali MV, Faridi MMA, Kaul PB, Ramachandran VC, et al. Clinical profile of Klebsiella septicaemia in neonates. Indian J Paediatr 1993; 60(4): 565-72.

Arora DR & Chugh TD. Klebocin types of Klebsiella pneumoniae isolated from normal and diarrhoeal stool. Indian J Med Res 1981; 72(1): 856.

Karbasizaed V, Badami N & Emtiazi G. Antimicrobial, heavy metal resistance and plasmid profile of coliforms isolated from nosocomial infections in a hospital in Isfahan, Iran. African Journal of Biotechnology 2003; 2(10): 379-83.

Wallace MR, Johnson A, Daniel M, Malde M & Yousif AA. Sequential emergence of multi-resistant Klebsiella. Pneumoniae in Bahrain. J Hosp Infect 1995; 31(4): 247-52.

Wang ll & Chen M. Surveillance for antimicrobial resistance among clinical isolates of gram-negative bacteria from intensive care unit patients in China 1996-2002. Diagnostic Microbiol Infect Dis 2005; 51(3): 201-8.

Ishii Y, Alba J, Kimura S, Shiroto K & Yamaguchi K. Evaluation of antimicrobial activity of Blactam antibiotics using E-test against clinical isolates from 60 medical centers in Japan. Antimicrob Agent 2005; 25(4): 296-301.

Koletar SL. Concepts in Antimicrobial Therapy. In: Mahon CR, Manoselis G. Textbook of Diagostic Microbiology. Chapter 3. Second Ed. Philadelphia. W.B Saunders Company. 2000; pp: 62-95

Baron EJ,Gold F, Sydney M, Editors. Diagnostic Microbiology. 8th ed. New York: Mosby Company; 1990.

Murray P, Baron R, Pfauer EJ, Tenoyer M, Yolken FC, Robert H, Editors. Manual of clinical Microbiology. 7th ed. Philadelphia: American Society for Microbiology; 1999.

Ferrari, M., Frnasier, C. M., Isetta, M. A. 1990. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. Immunological Methods, 131: 161-172.

Gerlier, D., Thomasset, N. 1986. Use of MTT colorimetric assay to measoure cell activation. . Journal of Immunological Methods, 94: 57-63.

Romijn JC, Verkoelen CF, Schroeder FH. Application of the MTT assay to human prostate cancer cell lines in vitro: establishment of test conditions and assessment of hormonestimulated growth and drug-induced cytostatic and cytotoxic effects. Prostate 1988; 12: 99-110.

Mohammadmehdi Fani and Jamshid Kohanteb; Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria Journal of Oral Science, Vol. 54, No. 1, 15-21, 2012

Agarry O.O., Olaleye M.T. and Bello-Michael, C.O. Comparative antimicrobial activities of aloe vera gel and leaf; African Journal of Biotechnology Vol. 4 (12), pp. 1413-1414

George D, MDS n Sham S. Bhat ; Comparative evaluation of the antimicrobial efficacy of aloe vera tooth gel and two popular commercial toothpastes: An in vitro study, General Dentistry Aloe vera tooth gel demonstrated enhanced antibacterial effect against S. mitis. MDS n Beena Antony, PhD 238 May/June 2009

Renisheya Joy Jeba Malar T, Johnson M, Nancy Beaulah S, Laju R S, Anupriya G, Renola Joy Jeba Ethal T; Anti-Bacterial And Antifungal Activity Of Aloe Vera Gel Extract International Journal of Biomedical and Advance Research 184 IJBAR (2012) 03(03) .

Published
2018-11-24
How to Cite
1.
Habibi G, Arjomandzadegan M, Tayeboon M, Didgar F, Sarmadian H, Sadrnia M, Mirhosseini F, Geravand S, Abdoli M. Comparison of antibacterial effects of a carrier produced in microemulsion system from aqueous extract of Aloe vera with selected antibiotics on Enterobacteriacea. Iran J Microbiol. 10(5):334-341.
Section
Original Article(s)