Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 10 5 2018 11 24 334 341 EN Ghasem Habibi Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Mohammad Arjomandzadegan Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Maryam Tayeboon Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Farshideh Didgar Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Hossein Sarmadian Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Maryam Sadrnia Department of Biology, Payame Noor University, Tehran, Iran Farid Mirhosseini Department of Chemistry, Faculty of Sciences, Arak University, Arak, Iran Somayeh Geravand Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran Mahboobeh Abdoli Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran 2018 04 01 2018 07 26 Background and Objectives: Antibiotics resistance has recently increased. The aim of this study was the evaluation of antibacterial efficacy of Aloe vera carrier produced in microemulsion system in comparison with ordinary antibiotics against some Enterobacteriacea. Materials and Methods: The aquatic extract of Aleo vera was produced by the Soxhlet method and a nonocarrier in the microemulsion system was prepared by two emulsifiers. The clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Shigella dysenteriae, Salmonella Typhimurium, Salmonella Paratyphi, Serratia marcescens, Proteus mirabilis, Enterobacter aerogenes, Citrobacter freundii and Morganella morganii were obtained from patients and were identified by microbiological methods. Diffusion disk was used for evaluation of antibacterial properties in comparison with selected ordinary antibiotics. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for tested materials were determined using MTT in the Micro Broth dilution method. Results: The results proved that effect of carrier on studied isolates is dependent on concentration level. The inhibitory effect of carrier in concentration of 15 µg/ml by 18 mm zone of inhibition for Klebsiella pneumoniae was comparable to Ceftazidime and Cefalothin. The lowest MIC and MBC determined by the Microbroth dilution method with MTT belonged to Klebsiella pneumoniae as 0.1 and 3 µg/ml and higher concentrations belonged to Enterobacter aerogenes at 7 and 15 µg/ml. The greatest effect of carrier of Aleo vera aquatic extract was observed for Klebsiella pneumoniae and the lowest effect belonged to Enterobacter aerogenes, Citrobacter freundii and Morganella morganii. Conclusion: It was concluded that the carrier of Aloe vera produced in microemulsion system was most effective and had equal effects in comparison with ordinary antibiotics against Enterobacteriacea. https://ijm.tums.ac.ir/index.php/ijm/article/view/1694 https://ijm.tums.ac.ir/index.php/ijm/article/download/1694/1074 https://ijm.tums.ac.ir/index.php/ijm/article/download/1694/1076