In vitro antibacterial effects of silver nanoparticles synthesized using Verbena officinalis leaf extract on Yersinia ruckeri, Vibrio cholera and Listeria monocytogenes

  • Narjes Sanchooli Department of Fisheries of Hamoun International Wetland Research Institute, University of Zabol, Zabol, Iran
  • Saeide Saeidi Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran
  • Hashem Khandan Barani Department of Fisheries of Hamoun International Wetland Research Institute, University of Zabol, Zabol, Iran
  • Esmael Sanchooli Department of Chemistry, University of Zabol, Zabol, Iran
Keywords: Antibacterial activity, Green synthesis, Verbena officinalis, Minimum inhibitory concentration

Abstract

Background and Objectives: The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by Verbena officinalis leaf extract against Yersinia ruckeri, Vibrio cholerae and Listeria monocytogenes.Materials and Methods: Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and V. officinalis leaf extract. The AgNPs were characterized by UV-visible spectrophotometer, scanning electron microscopy (SEM), and Fourier transform infrared spectrometer (FTIR). To determine minimum inhibitory concentration and test antibiogram of nanoparticle synthesized, broth micro dilution and agar well diffusion methods were used, respectively. Results: The zones of bacterial inhibition were 16 ± 0.5 and 9.16 ± 0.28 mm against Y. ruckeri and L. monocytogenes using 10 and 0.62 mg/mL AgNPs, respectively. Among the studied bacterial species, silver nanoparticles were more effective on Y. ruckeri and L. monocytogenes and less effective on V. cholerae. The highest MIC and MBC of AgNPs (2.5 and 5 mg/mL) were observed for V. cholerae. The lowest MIC and MBC of AgNPs (0.32 and 0.62 mg/mL) were observed for Y. ruckeri, respectively. The MIC and MBC of AgNPs were found to be 1.25 and 2.5 mg/mL for L. monocytogenes.Conclusion: The results clearly indicated that V. officinalis AgNPs have potential antimicrobial activity against Gram-positive and Gram-negative bacteria.

References

Argyropoulou CC, Daferera D, Tarantilis PA. Chemical composition of the essential oilfrom leaves of Lippia citriodora H.B.K. (Verbenaceae) at two developmental stages. Plant Geno Evol. 2007; 35: 831–837.

Baker C, Pradhan A, Akstis LP, Pochan DJ, Shah SI. Synthesis and Antibacterial Properties of Silver Nanoparticles", J Nanosci and Nanotechno. 2005; 5:244-249.

Banerjee P, Satapathy M, Mukhopahayay, A Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess. 2014; 1:2-10.

Ben Embarek, PK. Presence, detection, and growth of Listeria monocytogenes in seafoods. Int J Food Microbiol. 1994; 23: 17-34.

Gnanadesigan M, Anand M, Ravikumar S, Maruthupandy M, Ali, MS Vijayakumar V, Kumaraguru AK. Antibacterial potential of biosynthesized silver nanoparticles using Avicennia marina mangrove plant. Appl Nanosci. 2010; 2:143-147.

Hamouda T, Baker JR. Antimicrobial mechanism of action of surfactant lipid preparations in enteric gram-negative bacilli. J Appl Microbiol. 2000; 89:397-403.

Heidarpour F, Ghani WA, Ahmadun FR, Sobri S, Zargar M, Mozafari MR. Nano silver-coated polypropylene water filter: I. Manufacture by electron beam gun using a modified balzers 760 machine. Dig J Nanomater Biostruct. 2010; 5:787–796.

Jacob SJ, Finub JS, Narayanan A. Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line. Colloidsand surfaces B, Biointerfaces. 2012; 91:212-214.

Jain D, Daima KH, Kachhwaha S, Kothari SL. Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their anti microbial activities. Dig J Nanomat Biostruct. 2009; 4:723-27.

Jain N, Sharma M. Biodiversity of keratinophilic fungal flo¬ra in university campus, jaipur, India. Iran Journal Public Health. 2012; 41(11):27–33.

Karuppiah M, Rajmohan R. Green synthesis of silver nanoparticles using Ixora coccinea leaves extract. Material letter. 2013; 97:141–143.

Khalil MMH. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian J Chem. 2013; 1131–1139.

Klasen HJ. A historical review of the use of silver in the treatment of burns. I. early uses. Burns. 2000; 26:117-130.

Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B Biointerfaces. 2010; 76:50-56.

Kumarasamyraja D, Jeganathan, NS. Green synthesis of silver nanoparticle using aqueous extract of Acalypha indica and its antimicrobial activity. Int J Pharm and Bio Sci. 2013; 4: 469 – 476.

Logeswari P, Silambarasan S, Abraham J. Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc. 2015; 19(3):311-317.

Mariselvam R, Ranjitsing AJA, Usha R, Nanthini A, Kalirajan K, Padmalatha C, Mosae SP Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (family: Arecaceae) for enhanced antibacterial activity. Spectrochim Acta A. 2014; 129: 537–541.

Nafe A, Tasneem F, Ajit V, Ram P, Biogenic synthesis of silver nanoparticles using Scenedesmus abundans and evaluation of their antibacterial activity. J Nanoparticles. 2014; 1-6.

Namratha, N, Monica, PV. Synthesis of silver nanoparticles using Azadirachta indica (Neem) extract and usage in water purification. Asian J Pharm Tech. 2013; 3:170–174.

Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? a study of the Gram,negative bacterium Escherichia coli. Appl Environ Microbiol. 2007; 17:12-20.

Palanivelu J, Kunjumon MM, Anushree Suresh A, Ashwini Nair, A. Green Synthesis of Silver Nanoparticles from Dracaena mahatma Leaf Extract and its Antimicrobial Activity. J Pharm Sci Res. 2015; 7: 690-695.

Petrus EM, Tinakumari S, Chai LC, Ubong A, Tunung R, Elexson N, Chai LF, Son RA. study on the minimum inhibitory concentration and minimum bactericidal concentration of Nano Colloidal Silver on food-borne pathogens. Int food Res. 2011; 18: 55-66.

Rajesh S, Raja DP, Rathi, JM, Sahayaraj K. Biosynthesis of silver nanoparticles using Ulva fasciata(Delile) ethylacetate extract and its activity against Xanthomonas campestris Pv. malvacearum. J Biopest. 2012; 5:119-128.

Reid J, Klose KE. Vibrio cholerae and cholera: out of the water and into the host. FEMS Microbiol Review. 2002; 29: 125-139.

Ross Aj, Rucker Rr, Ewing WH. Description of a bacterium associated with redmouth disease of rainbow trout. J Microbiol. 1966; 12(4):763-770.

Roy K, Biswas S, Banerjee PC. Green Synthesis of Silver Nanoparticles by Using Grape (Vitis vinifera) Fruit Extract: Characterization of the Particles and Study of Antibacterial Activity. Res J Pharma Biol Chem Sci. 2013; 4(1): 1271-1278.

Ruparelia JP, Kumar A, Duttagupta SP, Diao M, Yao, M. Use of zero-valentir nanoparticles in inactivating microbes. water Res. 2009; 43:5243-51.

Saxena A, Tripathi RM, Singh RP. Biological synthesis of silver nanoparticles by using onion (allium cepa) extract and their antibacterial activity. Dig J Nanomater and Bios. 2010; 5: 427– 432.

Tobback E, Decostere A, Hermans K, Haesebrouck F, Chiers, K. Yersinia ruckeri infections in salmonid fish. J Fish dis. 2007; 30: 257–268.

Veerasamy R, Xin TZ, Gunasagaran S, Xiang TFW, Yang EFC, Jeyakumar N, et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc. 2011; 15:113-20.

Vijayakumar M, Priya K, Nancy FT, Noorlidah A, Ahmed AB. Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Industrial Crops and Products. 2013; 41:235-40.

Published
2019-02-10
How to Cite
1.
Sanchooli N, Saeidi S, Khandan Barani H, Sanchooli E. In vitro antibacterial effects of silver nanoparticles synthesized using Verbena officinalis leaf extract on Yersinia ruckeri, Vibrio cholera and Listeria monocytogenes. Iran J Microbiol. 10(6):400-8.
Section
Original Article(s)