Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 10 6 2019 02 10 400 408 EN Narjes Sanchooli Department of Fisheries of Hamoun International Wetland Research Institute, University of Zabol, Zabol, Iran Saeide Saeidi Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran Hashem Khandan Barani Department of Fisheries of Hamoun International Wetland Research Institute, University of Zabol, Zabol, Iran Esmael Sanchooli Department of Chemistry, University of Zabol, Zabol, Iran 2017 02 23 2018 06 05 Background and Objectives: The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by Verbena officinalis leaf extract against Yersinia ruckeri, Vibrio cholerae and Listeria monocytogenes. Materials and Methods: Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and V. officinalis leaf extract. The AgNPs were characterized by UV-visible spectrophotometer, scanning electron microscopy (SEM), and Fourier transform infrared spectrometer (FTIR). To determine minimum inhibitory concentration and test antibiogram of nanoparticle synthesized, broth micro dilution and agar well diffusion methods were used, respectively. Results: The zones of bacterial inhibition were 16 ± 0.5 and 9.16 ± 0.28 mm against Y. ruckeri and L. monocytogenes using 10 and 0.62 mg/mL AgNPs, respectively. Among the studied bacterial species, silver nanoparticles were more effective on Y. ruckeri and L. monocytogenes and less effective on V. cholerae. The highest MIC and MBC of AgNPs (2.5 and 5 mg/mL) were observed for V. cholerae. The lowest MIC and MBC of AgNPs (0.32 and 0.62 mg/mL) were observed for Y. ruckeri, respectively. The MIC and MBC of AgNPs were found to be 1.25 and 2.5 mg/mL for L. monocytogenes. Conclusion: The results clearly indicated that V. officinalis AgNPs have potential antimicrobial activity against Gram-positive and Gram-negative bacteria. https://ijm.tums.ac.ir/index.php/ijm/article/view/1289 https://ijm.tums.ac.ir/index.php/ijm/article/download/1289/1088