Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
-
Hashem Fakhre-Yaseri
,
Ali Baradaran-Moghaddam
,
Mehdi Shekaraby
,
Hamid Reza Baradaran
,
Seyed Kamran Soltani-Arabshahi
Abstract
Background and Objectives: The cytotoxin-associated gene (cag) pathogenicity island is reported to be a major virulence factor of Helicobacter pylori infection. It is previously reported that the cagA-positive strains are more virulent, so it can be postulated that the cagA-positive gastritis will be more severe and the serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody titer will be higher. The aim of this study was to compare the relationship between IgG and IgA anti-CagA antibody and the cagA gene expression in patients with dyspepsia. Serum samples obtained from 130 dyspeptic patients with positive H. pylori in histological and Geimsa staining were tested for serum IgG and IgA anti-CagA antibody using the enzyme-linked immunosorbent Assay. The expression of the cagA gene was determined using PCR on the biopsy samples, taken via endoscopy.
Results: In our material, the sensitivity of IgG anti-CagA antibody in identifying patients with a proven infection with the cagA-positive strains was 97.67%, and the negative likelihood ratios was 0.06. There was not significant correlation between serum IgA anti-CagA and the expression of the cagA gene among the dyspeptic patients.
Conclusion: The IgG antibody titer was significantly higher in our patients with the cagA-positive H. pylori strain. However, in daily practice, the level of the IgG antibody titer cannot predict whether or not an individual carries a cagA-positive H. pylori strain, because there is a major overlap in the IgG antibody titer between the cagA-positive and cagA-negative patients.
Atherton JC, Blaser M. Co-adaptation of Helicobacter pylori and humans: ancient history, modern implications. J Clin Invest 2009;119:2475-2487.
Atherton JC. The pathogenesis of Helicobacter pylori-induced gastro-duodenal disease. Annu Rev Pathol Mech Dis 2006;1:69-96.
Kim SS, Ruiz VE, Carroll JD, Moss SF. Helicobacter pylori in the pathogenesis of gastric cancer and gastric lymphoma. Cancer Lett 2011;305:228-238.
Baghaei K, Shokrzadeh L, Jafari F, Dabiri H, Yamaoka Y, Bolfion M, et al. Determination of Helicobacter pylori virulence by analysis of the cag pathogenicity island isolated from Iranian patients. Dig Liver Dis 2009;41:634-638.
Gatti LL, Fagundes e Souza EK, Leite KR, Bastos EL, Vicentini LR, Silva LC, et al. cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients. Diagn Microbiol Infect Dis 2005;51:231-235.
Ozen A, Ertem D, Pehlivanoglu E. Natural history and symptomatology of Helicobacter pylori in childhood and factors determining the epidemiology of infection. J Pediatr Gastroenterol Nutr 2006;42:398-404.
Altmana E, Chandan V, Harrison BA, Pitab RV, Li J, KuoLee R, Chen W, Bencomo VV. Design and immunological properties of Helicobacter pylori glycoconjugates based on a truncated lipopolysaccharide lacking Lewis antigen and comprising an α-1,6-glucan chain. Vaccine 2012;30:7332-7341.
Abasiyanika MF, Tuncb M, Saliha BA. Enzyme immunoassay and immunoblotting analysis of Helicobacter pylori infection in Turkish asymptomatic subjects. Diagn Microbiol Infect Dis 2004;50:173-177.
Lepper PM, Moricke A, Vogt K, Bode G, Trautmann M. Comparison of different criteria for interpretation of immunoglobulin G immunobloatting results for diagnosis of Helicobacter pylori infection. Clin Diagn Lab Immunol 2004;11:569-576.
Moayyedi P, Duffett S, Braunhottz D, Mason S, Richards ID, Dowell AC, et al. The Leed´s Dyspepsia Questioner (LDQ); a valid tool for measuring the presence and severity of dyspepsia. Alimen Pharmacol Ther 1998;12:1257-1262.
Mukhopadhyay AK, Kersulyte D, Yong Jeong J, Datta S, Ito Y, et al. Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India. J Bacteriol 2000;182:3219-3227.
Oluwasola AO, Otegbayo JA, Ola SO, Ebili HO, Afolabi AO, Odaibo GN. Correlation of serum Anti–Helicobacter Pylori immunoglobulin A(IgA) with histological parameters of chronic gastritis in Ibadan, Nigeria. Ann Ib Postgrad Med 2012;10:18-24.
Schӧttker B, Adamu MA, Weck MN, Brenner H. Helicobacter pylori infection is strongly associated With gastric and duodenal ulcers in a large prospective study. Clin Gastroentrol Hepatol 2012;10:487-493.
Perez-Perez GI, Maw AM, Link LF, Gunn J, Bowers AL, Minano C, et al. Longitudinal analysis of serological responses of adults to Helicobacter pylori antigens. J Infect Dis 2010;202:916-923.
Azuma T, Yamazaki S, Yamakawa A, Ohtani M, Muramatsu A, Suto H, et al. Association between diversity in the Src homology 2 domain-containing tyrosine phosphatase binding site of Helicobacter pylori CagA protein and gastric atrophy and cancer. J Infect Dis 2004;189:820-827.
Talley NJ, Vakil N. Guidelines for the management of dyspepsia. Am J Gastroentrol 2005;100:2324-2337.
| Files | ||
| Issue | Vol 9 No 2 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| cagA IgG anti-CagA IgA anti-CagA Dyspepsia | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
