Detection of lytA, pspC, and rrgA genes in Streptococcus pneumoniae isolated from healthy children
,
Abstract
Background and Objectives: Many surface proteins are implicated in nasopharyngeal colonization and pathogenesis of Streptococcus pneumoniae. Some of these factors are candidate antigens for protein based vaccines. New vaccine designs focus on the surface proteins (e. g., pspA and pspC) and also cytolysin, and pneumolysin. In this study, 3 key virulence genes, lytA, pspC, and rrgA, which encoded surface proteins, were detected among S. pneumoniae isolates.
Materials and Methods: A total of 260 nasopharyngeal swabs were collected from healthy children under 6 years old attending day care centers in Mashhad, Iran. Isolates of S. pneumoniae were confirmed by optochin susceptibility and colony appearance and also by PCR for cpsA gene. The presence of lytA, pspC, and rrgA genes were also detected by PCR.
Results: A total of 59 isolates were confirmed as S. pneumoniae. Among these isolates, 50 (84.74%), 19 (32.20%), and 2 (3.38%) were positive for lytA, rrgA, and pspC genes respectively. The presence of these genes among S.pneumoniae isolates were as follows: 1) rrgA, lytA, pspC (1 isolate), 2) rrgA, lytA(17isolates), 3) pspC (2 isolate), 4) lytA (50 isolates).
Conclusion: cpsA gene was specific for detection of S. pneumoniae isolates which were colonized in nasopharynx. The lytA gene was the most frequent gene among the S. pneumoniae isolates, and combination of rrgA, lytA was the most observed pattern. Thus, it is important for future monitoring of vaccine formulation in our country.
Neves FP, Pinto TC, Correa MA, dos Anjos Barreto R, de Souza Gouveia Moreira L, Rodrigues HG, et al. Nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among children from Brazil before the introduction of the 10-valent conjugate vaccine. BMC Infect Dis 2013;13:318.
Sakai F,Talekar SJ, Klugman KP, Vidal JE. Expression of Streptococcus pneumoniae virulence-related genes in the nasopharynx of healthy children. PLoS One 2013;8: e67147.
Le Polain de Waroux O, Flasche S, Prieto-Merino D, Edmunds WJ. Age-dependent prevalence of nasopharyngeal carriage of Streptococcus pneumoniae before conjugate vaccine introduction: A prediction model based on a meta-analysis. PLoS One 2014;9:e86136.
Voss S, HallstromT, Saleh M, Burchhardt G, Pribyl T, Singh B, et al. The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin. J Biol Chem 2013; 288: 15614-27.
Mitchell AM, Mitchell TJ. Streptococcus pneumoniae:virulence factors and variation. Clin Microbiol Infect 2010; 16:411-418.
Ricci S, Gerlini A, Pammolli A, Chiavolini D, Braione V, Tripodi SA, et al. Contribution of different pneumococcal virulence factors to experimental meningitis in mice. BMC Infect Dis 2013; 13: 444.
Dave S, Carmicle S, Hammerschmidt S, Pangburn MK, McDaniel LS. Dual roles of PspC, a surface protein of Streptococcus pneumoniae, in binding human secretory IgA and factor H. J Immunol 2014;173:471-477.
Iannelli F, Oggioni MR, Pozzi G. Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae. Gene 2002;284: 63–71.
Kurola P (2011). Role of pneumococcal virulence genes in the etiology of respiratory tract infection and biofilm formation. University of Oulu, Oulu-Finland.
Orsolya D (2004). Epidemiology of Streptococcus pneumoniae: Molecular characterisation, antibiotic sensitivity and serotyping of hungarian isolates. Semmelweis University, Budapest.
Whatmore AM, Dowson CG. The autolysin- encoding gene (lytA) of Streptococcus pneumoniae displays restricted allelic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes. Infect Immun 1999;67:4551–4556.
LeMieuxJ, Hava DL, BassetA, CamilliA. RrgA,rrgB and rrgC components of a multi subunit pilus encoded by the Streptococcus pneumoniae rlrA pathogenicity islet. Infect Immun 2006;74: 2453–2456.
Nelson AL, RiesJ, Bagnoli F, Dahlber S, Falker S, Rounioja S, et al. RrgA is a pilus-associated adhesin in Streptococcus pneumoniae. Mol Microbiol 2007;66:329–340.
Mousavi SF, Nobari S, Rahmati Ghezelgeh F, Lyriai H, JalaliP, Shahcheraghi F, et al.Serotyping of Streptococcus pneumoniae isolated from Tehran by Multiplex PCR: Are serotypes of clinical and carrier isolates identical? Iran J Microbiol 2013;5:220-226.
SuzukiN, YuyamaM, Maeda S, Ogawa H, Mashiko K, Kiyoura Y. Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniae. J Med Microbiol 2006;55:709–714.
Iannelli F,Chiavolini D, Ricci S,Oggioni MR, Pozzi G.Pneumococcal surface protein C contributes to sepsis caused by Streptococcus pneumoniae in mice. Infect Immun 2004;72:3077-3080.
Hava DL, Hemsley CJ, Camilli A. Transcriptional regulation in the Streptococcus pneumoniae rlrA pathogenicity islet by RlrA. J Bacteriol 2003;185:413-421.
Basset A, Turner KH, BoushE, Sayeed S, Dove SL, MalleyR. Expression of the type 1 pneumococcal pilus is bistable and negatively regulated by the structural component RrgA. Infect Immun 2011;79: 2974–2983.
Park HK, Lee SJ,Yoon JW, ShinJW, Shin HS, Kook JK, et al. Identification of the cpsA gene as a specific marker for the discrimination of Streptococcus pneumoniae from viridans group streptococci. J Med Microbiol 2010; 59:1146–1152.
Ramos Sevillano E, Rodriguez Sosa C, Diez Martinez R, Gimenez MJ, Olmedillas E, Garcia P, et al. Macrolides and β-Lactam antibiotics enhance C3b deposition on the surface of multidrug-resistant Streptococcus pneumoniae strains by a Lyt A autolysin-dependent mechanism. Antimicrob Agents Chemother 2012; 56: 5534–5540.
Rosenow C ,Ryan P, Weiser JN, Jonson S, Font P,Ortqvist A, Masure HR. Contribution of novel cholin-binding proteins to adherence, colonization and immunogenicity of Stereptococcus pneumoniae. Mol Microbiol 1997;25:819-829.
Wizemann TM, Adamou JE, LangermannS. Adhesins as targets for vaccine development. Emerg Infect Dis 1999;5:395-403.
| Files | ||
| Issue | Vol 7 No 3 (2015) | |
| Section | Articles | |
| Keywords | ||
| Streptococcus pneumoniae lytA pspC rrgA children | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
