Plasma surface modificationas a new approach to protect urinary catheteragainst Escherichia coli biofilmformation
Abstract
Background and Objectives: Biomaterials are widely used in medical devices such as urinary catheters. One of the main problems associated with long term using of the urinary catheters is biofilm formation on their surfaces. Many techniques have been presented to reduce the biofilm formation. One of the most revolutionary techniques allowing such surface fictionalization is plasma surface modification.
Materials and Methods: In this study, a glow discharge plasma (GDP) effect on Escherichia coli biofilm formation on the surface of urinary catheter in the pressure of 1.6 × 10-1 Torr of nitrogen, discharge voltage about 1.2 kV and current of 150 mA for 20 minutes has been investigated. Crystal violet binding assay and sonication method were performed in order to evaluate the amount of biofilm formation on tested biomaterials.
Results: Characterization of modified surfaces by Attenuated Total Reflectance Fourier Transform Infrared Spectrometry (ATR-FTIR) and atomic force microscopy (AFM) revealed a noticeable change in hydrophobicity and roughness of catheter surfaces achieved by nitrogen plasma. The results of crystal violet binding assay and sonication method showed that the amount of biofilm formation on modified surface was about 86% less than the pristine sample.
Conclusion: Plasma surface modification can reduce the risk of infections in patients with long-term use of urinary catheters.
Langer R, Peppas NA. Advances in biomaterials, drug delivery, and bionanotechnology. AIChE J 003;49:2990-2006
Aronsson BO, Lausmaa J, Kasemo B. Glow discharge plasma treatment for surface cleaning and modification of metallic biomaterials. J Biomed Mater Res 1997; 35:49-73.
Nichols RL, Raad II. Management of bacterial com- plications in critically ill patients: surgical wound and catheter-related infections. Diagn Microbiol Infect Dis 1999; 33: 121-130.
Watnick P, Kolter R. Biofilm, city of microbes. J Bacte- riol 2000; 182: 2675- 2679.
Chandki R, Banthia P, Banthia R. Biofilms: A microbi- al home. J Indian Soc Periodontol 2011; 15: 111-114.
Davies D. Understanding biofilm resistance to antibac- terial agents. Nat Rev Drug Discov 2003; 2: 114-122.
Donlan RM, Costerton JW. Biofilms: survival mecha- nisms of clinically relevant microorganisms. Clin Mi- crobiol Rev 2002; 15: 167-193.
Mah TF, O'Toole GA. Mechanisms of biofilm resis- tance to antimicrobial agents. Trends Microbiol 2001;9: 34-39.
Trautner BW, Darouiche RO. Role of biofilm in cathe- ter-associated urinary tract infection. Am J Infect Con- trol 2004; 32: 177-183.
Bazaka K, Jacob MV, Crawford RJ, Ivanova EP. Plas- ma-assisted surface modification of organic biopoly- mers to prevent bacterial attachment. Acta Biomater 2011; 7: 2015-2028.
Flemming HC, Ridgway H (2009). Biofilm control: Conventional and alternative approaches. In: Marine and Industrial Biofouling. Ed, HC Flemming, PS Mur- thy, R Venkatesan, KE Cooksey. Springer, 1st ed. USA, pp. 103-117.
Simões M, Simões LC, Vieira MJ. A review of current and emergent biofilm control strategies. LWT-Food Sci Technol 2010; 43: 573-583.
Cortés ME, Bonilla JC, Sinisterra RD (2011). Biofilm formation, control and novel strategies for eradication. In: Science against microbial pathogens: Communicat- ing current research and technological advances. Ed, A Mendez-Vilas. Formatex Research Center, Spain, Vol., pp. 896-905.
Estrela AB, Abraham WR. Combining biofilm-con- trolling compounds and antibiotics as a promising new way to control biofilm infections. Pharmaceuticals 2010; 3: 1374-1393.
Bazaka K, Jacob MV, Crawford RJ, Ivanova EP. Ef- ficient surface modification of biomaterial to prevent biofilm formation and the attachment of microorgan- isms. Appl Microbiol Biotechnol 2012; 95: 299-311.
Lawson MC, Hoth KC, Deforest CA, Bowman CN, Anseth KS. Inhibition of Staphylococcus epidermidis biofilms using polymerizable vancomycin derivatives. Clin Orthop Relat Res 2010; 468: 2081-2091.
Sodhi RNS, Sahi VP, Mittelman MW. Application of electron spectroscopy and surface modification tech- niques in the development of anti-microbial coatings for medical devices. J Electron Spectros Relat Phe- nomena 2001; 121: 249-264.
Rodrigues LR. Inhibition of bacterial adhesion on medical devices. Adv Exp Med Biol 2011; 715: 351-367.
Cheruthazhekatt S, Černák M, Slavíček P, Havel J. Gas plasmas and plasma modified materials in medicine. J Appl Biomed 2010; 8: 55-66.
Pompl R, Jamitzky F, Shimizu T, Steffes B, Bunk W, Schmidt HU, et al. The effect of low-temperature plas- ma on bacteria as observed by repeated AFM imaging. New J Phys 2009; 11: 115023.
Moman RM, Najmaldeen H. The bactericidal efficacy of cold atmospheric plasma technology on some bacte- rial strains. Egypt Acad J Biol Sci 2010; 2: 43-47.
Wilson DJ, Rhodes NP, Williams RL. Surface mod- ification of a segmented polyetherurethane using a low-powered gas plasma and its influence on the ac- tivation of the coagulation system. Biomaterials 2003;24: 5069-5081.
Roth JC (2004). Prospective industrial applications of the one atmosphere uniform glow discharge plasma. Conf Rec. 39th IEEE IAS Annu Meeting. vol 1, pp. 223.
Kelly-Wintenberg K, Sherman DM, Tsai PPY, Gadri RB, Karakaya F, Chen Z, et al. Air filter sterilization using a one atmosphere uniform glow discharge plas- ma (the volfilter). IEEE Trans Plasma Sci 2000; 28: 64-71.
Adetunji VO, Isola TO. Crystal violet binding assay for assessment of biofilm formation by Listeria monocyto- genes and Listeria spp on wood, steel and glass surfac-es. Global Veterinaria 2011; 6: 6-10.
Bjerkan G, Witsø E, Bergh K. Sonication is superior to scraping for retrieval of bacteria in biofilm on titanium and steel surfaces in vitro. Acta Orthop 2009; 80: 245-250.
Balazs DJ, Triandafillu K, Chevolot Y, Aronsson BO, Harms H, Descouts P, et al. Surface modification of PVC endotracheal tubes by oxygen glow discharge to reduce bacterial adhesion. Surf Interface Anal 2003 ;35: 301-309.
Wang IW, Anderson JM, Jacobs MR, Marchant ER.Adhesion of Staphylococcus epidermidis to biomedical polymers: contributions of surface thermodynamics and hemodynamic shear conditions. J Biomed Mater Res 1995; 29: 485-493.
Files | ||
Issue | Vol 8 No 4 (2016) | |
Section | Articles | |
Keywords | ||
Plasma treatment Glow discharge plasma surface Modification biofilm catheter |
Rights and permissions | |
![]() |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |