Evaluation of antibacterial efficiency of chitosan and chitosan nanoparti- cles on cariogenic streptococci: an in vitro study
-
Azam Aliasghari
,
Mohammad Rabbani-Khorasgani
,
Sedigheh Vaezifar
,
Fateh Rahimi
,
Habibollah Younesi
,
Maryam Khoroushi
Abstract
Background and Objectives: The most prevalent and worldwide oral disease is dental caries that affects a significant pro- portion of the world population. There are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. Therefore new methods are needed for better management of this important challenge. Chitosan is a natural and non-toxic polysaccharide with many biological applications, particu- larly as an antimicrobial agent. Chitosan nanoparticle is a bioactive and environment friendly material with unique physico- chemical properties. The aim of the present study was to investigate the antimicrobial effect of chitosan and nano-chitosan on the most important cariogenic streptococci.
Materials and Methods: For evaluation of antimicrobial effect of chitosan and nano-chitosan against oral streptococci broth micro-dilution method was carried out for four bacterial species; Streptococcus mutans, Streptococcus sobrinus, Streptococ- cus sanguis and Streptococcus salivarius. Also the effect of these materials on adhesion of above bacteria was evaluated. One-way ANOVA and post hoc Tukey test were used for statistical analysis.
Results: The MICs of chitosan for S. mutans, S. sanguis, S. salivarius and S. sobrinus were 1.25, 1.25, 0.625 and 0.625 mg/ mL, respectively. The MIC of chitosan nanoparticle for S. mutans, S. salivarius and S. sobrinus was 0.625 mg/mL and for S. sanguis was 0.312 mg/mL. Chitosan and chitosan nanoparticles at a concentration of 5 mg/mL also reduced biofilm forma- tion of S. mutans up to 92.5% and 93.4%, respectively.
Conclusion: T
e results of this study supported the use of chitosan and chitosan nanoparticles as antimicrobial agents against cariogenic Streptococci.
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| Issue | Vol 8 No 2 (2016) | |
| Section | Articles | |
| Keywords | ||
| Chitosan Chitosan nanoparticles Dental caries Oral streptococci | ||
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