Articles

Comparison of Butyric acid concentrations in ordinary and probiotic yogurt samples in Iran

Abstract

Background and Objectives: Butyric acid has many applications in chemical, food and pharmaceutical industries. Applications of butyric acid are as an additive to food, flavorings, varnishes, perfumes, pharmaceuticals and disinfectants. Butyric acid concentrations have positive impact on the quality control of milk, yogurt and other probiotic dairy products. The present investigation was undertaken to determine and compare the concentrations of butyric acid (C4) in the ordinary and probiotic yogurt samples by GC method.
Materials and Methods: Probiotic yogurt samples were prepared under laboratory scale conditions using two different commercial starters ABY1 and 211, while ordinary yogurt samples lacked the probiotic starter cultures. All samples were analyzed in duplicate, for C4 concentrations by gas chromatography after day 1, 2, 10 and 20 of production, during storage at 4ºC. The results were analyzed using ANOVA and Duncan test.
Results: The level of the mentioned fatty acid in ABY1 yogurt sample was significantly higher (0.2%) than in 211 samples (0.17%). These values were significantly lower in ordinary yogurt samples and only 0.07% was recorded in these samples on first day of storage which decreased gradually during storage. The level of reduction in the yogurt samples tested during different time intervals was not similar in all the examined samples, and some showed enhanced reduction than other samples.
Conclusions: Compared to ordinary yogurt samples, probiotic yogurt samples used in study showed higher levels of butyric acid with increased shelf life.

Evers, J. M. (2003). Determination of free fatty acids in milk using the BDI method-some practical and theoretical aspects. Int Dairy J, 13, 111-121.

Moreau NM, Delepee R, Maume D, LeBizec B, Nguyen PG, Champ MM, et al. Rapid measurement of C-enrichment of acetic, propionic and butyric acids in plasma with solid phase microextraction coupled to gas- chromatography mass spectrometry .Analytica Chimica Acta 2004; 512: 305-310.

Filípek J, Dvořák R. Determination of the Volatile Fatty Acid Content in the Rumen Liquid: Comparison of Gas Chromatography and Capillary Isotachophoresis. Acta Vet Brno 2009; 78: 627-633.4. Akaln S, Gonc S, Unal G. Functional properties of bioactive components of milk fat in metabolism. Pak J Nut 2006; 5: 194-197.

Lupton JR. Microbial degradation products influence colon cancer risk: the butyrate controversy. J Nutr 2004;134: 479-482.

Association of Official Analytical Chemists. (1995).In Official methods of analysis (16th ed, p. 250). Gaithersburg, MD, USA: AOAC International 536, 858.

Watson R, Wright CJ, McBurney T, Taylor AJ, Linforth RST. Influence of harvest data and light integral on the development of strawberry flavor compounds. J Exp Bot 2002; 53: 2121-2129.

Molkentin J, Prech D. Comparison of gas chromatographic methods for analysis of butyric acid in milk fat and fats containing milk fat. Z Lebensm Unters Forsch A 1998; 206: 213-216.

Ming-Hua Y, Youk-Meng C. A rapid gas chromatographic method for direct determination of short-chain (C2–C12) volatile organic acids in foods. food Chem. 2001; 75: 101-108.

Yadav H, Shalini J and Sinha PR. Production of free fatty acids and conjugated linoleic acid in probiotic dahi containing L. acidophilus and L. casei during fermenta-tion and storage. Int Dairy J 2007; 17: 1006-1010.

Yang MH, Choong YM. A rapid gas chromatographic method for direct determination of short-chain (C2– C12) volatile organic acids in foods. Food Chem 2001;75: 101-108.

Van Immerseel F, Ducatelle R, De Vos M, Boon N, Van De Wiele T, Verbeke K, Rutgeerts P, Sas B, Louis P. Butyric acid-producing anaerobic bacteria as a novel probiotic treatment approach for inflammatory bowel disease. J Med Microbiol 2010; 59: 141-143.

Alosno C, Gilliand G. Production of free conjugated linileic acid by lactobacillus acidiphilus and lactobacillus casei of human origin. J Dairy Sci 2003; 86: 1941-1946.

Coskun JK, Ondul L. Free fatty acid accumulation by mesophilic lactic acid bacteria in cold stored milk. J Microbiol 2004; 42: 133-138.

Lamoureux L, Roy D, Gauthier SF. Production of oligosaccharides in yogurt containing Bifidobacteria and yogurt cultures. J Dairy Sci 2002; 85: 1058-1069.

Daly C, Davis R. The biotechnology of lactic acid bacteria with emphasis on applications in food safety and human health. Agri. food Sc 1998; 7: 251-264.

Centeno JA, Tomillo FJ, Fenández-García E, Gaya P, Nuňez M. Effect of wild strains of Lactococcus lactis on the volatile profile and the sensory characteristics of Ewes’ raw milk cheese. J Dairy Sci 2002; 85: 3164-3172.

Sheu BS, Wu JJ, Lo CY. Impact of supplement with Lactobacillus and Bifidobacterium containing yogurt on triple therapy for H. pylori eradication. The Aliment Pharmacol 2002; 16: 1669-1675.

Filipek JM, Dvorak R. Determination of the volatile fatty acid content in the rumen liquid: comparison of gas chromatography and capillary isotachophoresis. Acta Vet Brno 2009; 78: 627-633.

Li W, Han HJ, Zhang CH. Continuous butyric acid production by corn stalk immobilized Clostridium thermobutyricum cell. Afr J Microbiol Res 2011; 5:661-666.

Beshkova D, Simova E, Frengova G, Simov Z.Production of flavour compounds by yogurt starter cultures. J Ind Microbiol Biotech 1998; 20: 180-186.

Ogata T., Kingako M, Aeshima TY, Teraguchi S, Fukuwatari Y, Ishibashi N, Hayasawa H, Fujisawa T, Lino H . Microb Eco. Health and Dis 1999; 11: 41-46.

Adhikari K, Grün IU, Mustapha A, Fernando LN.Changes in the profile of organic acids in plain set and stirred yogurts during manufacture and refrigerated storage. J Food Quality 2001; 13: 78-84.

Wei MC, Chang CT, Jen JF. Determination of organic acids in fermentation products of milk with high performance liquid chromatography. Chromatographia 2001; 54: 601-605.

Files
IssueVol 4 No 2 (2012) QRcode
SectionArticles
Keywords
Probiotic butyric acid gas chromatography starter cultures yogurt

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Vaseji N, Mojgani N, Amirinia C, Iranmanesh M. Comparison of Butyric acid concentrations in ordinary and probiotic yogurt samples in Iran. Iran J Microbiol. 1;4(2):87-93.