Capsular serotype and antibiotic resistance of group B streptococci isolated from pregnant women in Ardabil, Iran
-
E Jannati
,
M Roshani
,
M Arzanlou
,
S Habibzadeh
,
G Rahimi
,
R Shapuri
Abstract
Background and Objectives: Group B Streptococci (GBS) is a major cause of neonatal and maternal infections. The aim of this study was to determine the serotype distribution and antibiotic resistance profile of GBS strains isolated from pregnant women in Ardabil.
Materials and Methods: Antibiotic resistance of 56 GBS isolates was investigated using E-test strips and disk-diffusion method. Serotyping was performed using capsular antiserum.
Results: The results of MIC tests showed all isolates were susceptible to ampicillin, vancomycin and penicillin. One isolate (1.7%) showed reduced susceptibility pattern to penicillin (MIC; 0.25 µg/ml). There were 3 (5.3%) isolates semi-sensitive (0.25-1 µg/ml) to erythromycin (2; 0.5 µg/ml and 1; 0.38 µg/ml) and 2 (3.5%) isolates to clindamycin (1; 0.5 µg/ml, 1;0.38 µg/ml). Additionally, 2 (3.5%) isolates were resistant to clindamycin (1; 16 µg/ml, 1; 2 µg/ml). According to the disk diffusion test, 47 (83.9%), 8 (14.2%) and 7 (12.5%) isolates were resistant to Co-trimoxazole, ciprofloxacin and ceftriaxone respectively. Serotypes V (19.6%), II (12.5%) and IV (12.5%) were the most frequent followed by serotypes III (10.7%) and VI (10.7%), Ib (8.9%), Ia (7/1%), VII (5/3%) and VIII (5/3%); 7.1% of strains were nontypeable.
Conclusions: In this study, most isolates were sensitive to common antibiotics, but increased resistance to other antibiotics indicates the importance of monitoring of antibiotic resistance in group B streptococci over time.
Koenig JM, Keenan WJ. Group B streptococcus and early-onset sepsis in the era of maternal prophylaxis. Pediatr Clin North Am 2009; 56: 689-708, Table of Contents.
Edwards MS, Baker CJ. Group B streptococcal infections in elderly adults. Clin Infect Dis 2005; 41: 839-847.
Picard FJ, Bergeron MG. Laboratory detection of group B Streptococcus for prevention of perinatal disease. Eur J Clin Microbiol Infect Dis 2004; 23: 665-671.
Schrag SJ, Zell ER, Lynfield R, Roome A, Arnold KE, Craig AS, et al: A population-based comparison of strategies to prevent early-onset group B streptococcal disease in neonates. N Engl J Med 2002; 347: 233-239.
Schrag S, Gorwitz R, Fultz-Butts K, Schuchat A.Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC. MMWR Recomm Rep 2002; 51: 1-22.
Schauf V, Deveikis A, Riff L, Serota A: Antibiotic- killing kinetics of group B streptococci. J Pediatr 1976;89: 194-198.
Chu YW, Tse C, Tsang GK, So DK, Fung JT, Lo JY. Invasive group B Streptococcus isolates showing reduced susceptibility to penicillin in Hong Kong. J Antimicrob Chemother 2007; 60: 1407-1409.
Dahesh S, Hensler ME, Van Sorge NM, Gertz RE, Jr., Schrag S, Nizet V, et al. Point mutation in the group B streptococcal pbp2x gene conferring decreased susceptibility to beta-lactam antibiotics. Antimicrob Agents Chemother 2008; 52: 2915-2918.
Kimura K, Suzuki S, Wachino J, Kurokawa H, Yamane K, Shibata N, et al. First molecular characterization of group B streptococci with reduced penicillin susceptibility. Antimicrob Agents Chemother 2008; 52:2890-2897.
Nagano N, Nagano Y, Kimura K, Tamai K, Yanagisawa H, Arakawa Y. Genetic heterogeneity in pbp genes among clinically isolated group B Streptococci with reduced penicillin susceptibility. Antimicrob Agents Chemother 2008; 52: 4258-4267.
Lee BK, Song YR, Kim MY, Yang JH, Shin JH, Seo YS, et al. Epidemiology of group B streptococcus in Korean pregnant women. Epidemiol Infect 2010; 138:292-298.
Wang YH, Su LH, Hou JN, Yang TH, Lin TY, Chu C, et al. Group B streptococcal disease in nonpregnant patients: emergence of highly resistant strains of serotype Ib in Taiwan in 2006 to 2008. J Clin Microbiol 2010; 48: 2571-2574.
Lancefield RC, Freimer EH. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966; 64: 191-203.
Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL.Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J Clin Microbiol 2007; 45: 2929-2936.
Jennings HJ. Capsular polysaccharides as vaccine candidates. Curr Top Microbiol Immunol 1990; 150:97-127.
Johri AK, Paoletti LC, Glaser P, Dua M, Sharma PK, Grandi G, et al. Group B Streptococcus: global incidence and vaccine development. Nat Rev Microbiol 2006; 4:932-942.
Baker CJ, Barrett FF. Group B streptococcal infections in infants. The importance of the various serotypes. JAMA 1974; 230: 1158-1160.
Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis 2010; 10: 336.
Habibzadeh S, Arzanlou M, Jannati E, Asmar M, fardiazar Z. Maternal carriage of group B Streptococcus in Ardabil, prevalence and antimicrobial resistance. J Ardabil Univ Med Sci 2010; 10: 14-20.
Berkowitz K, Regan JA, Greenberg E. Antibiotic resistance patterns of group B streptococci in pregnant women. J Clin Microbiol 1990; 28: 5-7.
Dhanoa A, Karunakaran R, Puthucheary SD. Serotype distribution and antibiotic susceptibility of group B streptococci in pregnant women. Epidemiol Infect 2010;, 138: 979-981.
Garland SM, Cottrill E, Markowski L, Pearce C, Clifford V, Ndisang D, et al. Antimicrobial resistance in group B streptococcus: the Australian experience. J Med Microbiol 2011; 60: 230-235.
Mansouri S, Ghasami E, Shahabi Najad N. Vaginal colonization of group B Streptococci during late pregnancy in southeast of Iran: Incidence, serotype distribution and susceptibility to antibiotics. J Med Sciences 2008; 8: 574-578.
Simoes JA, Aroutcheva AA, Heimler I, Faro S.Antibiotic resistance patterns of group B streptococcal clinical isolates. Infect Dis Obstet Gynecol 2004; 12:1-8.
Barnes P, Waterworth PM. New cause of penicillin treatment failure. Br Med J 1977; 1: 991-993.
Uh Y, Jang IH, Yoon KJ, Lee CH, Kwon JY, Kim MC. Colonization rates and serotypes of group B streptococci isolated from pregnant women in a Korean tertiary hospital. Eur J Clin Microbiol Infect Dis 1997;16: 753-756.
Shabayek SA, Abdalla SM, Abouzeid AM. Vaginal carriage and antibiotic susceptibility profile of group B Streptococcus during late pregnancy in Ismailia, Egypt. J Infect Public Health 2009; 2: 86-90.
Boswihi SS, Udo EE, Al-Sweih N. Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait. J Med Microbiol 2012; 61: 126-131.
Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P. Maternal carriage and neonatal colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iranian J Med Sciences 2007; 32: 177-181.
Mikamo H, Johri AK, Paoletti LC, Madoff LC,Onderdonk AB. Adherence to, invasion by, and cytokine production in response to serotype VIII group B Streptococci. Infect Immun 2004; 72: 4716-4722.
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| Issue | Vol 4 No 3 (2012) | |
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| Antibiotic resistance Group B Streptococci Serotype | ||
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