The effect of bacteria, enzymes and inulin on fermentation and aerobic stability of corn silage
Abstract
Background and Objectives: Ensiling is a conservation method for forage crops. It is based on the fact that anaerobe lactic acid bacteria (LAB) convert water-soluble carbohydrates into organic acids. Therefore, pH decreases and the forage is preserved. The aim of this study was to isolate special kinds of lactic acid bacteria from silage and to study the effect of bacteria, inulin and enzymes as silage additives on the fermentation and aerobic stability of the silage.
Materials and Methods: The heterofermentative LAB were isolated from corn silages in Broujerd, Iran and biochemically characterized. Acid tolerance was studied by exposure to acidic PBS and growth in bile salt was measured by the spectrophotometric method.
Results: The results of molecular analysis using 16SrDNA sequences showed that the isolates belonged to Lactobacillus and Enterococcus genera. To enhance stability in acidic environment and against bile salts, microencapsulation with Alginate and Chitosan was used. The Lactobacillus plantarum strains were used as control. The inoculants (1 × 107 cfu/g) alone or in combination with inulin or in combination with enzymes were added to chopped forages and ensiled in 1.5-L anaerobic jars.
Conclusion: Combination of the isolates Lactobacillus and Enterococcus with inulin and enzymes can improve the aerobic stability of corn silage.
Holzapfel WH, Haberer P, Geisen R, Schillinger U.Taxonomy and important features microorganisms in food and nutrition. Am J Clin Nutr 2001. 73(suppl):365S-73S.
Cai Y, Benno Y, Ogawa M, Ohmomo S, Kumai S, Nakase K. Influence of Lactobacillus spp. from an inoculant and Weissella and Leuconostoc spp. From forage crops on silage fermentation. Appl Environ Microbiol 1998; 64: 2982-2987.
Weinberg Z G, Ashbell G, Hen Y, Azrieli A. The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages. J Appl Bacteriol 1993; 75: 512-518.
Kung. Jr L, Tung RS, Maciorowski KG, Buffum K, Aimutis WR. Effects of plant cell-wall–degrading enzymes and lactic acid bacteria on silage fermentation and composition. J Dairy Sci 1991. 74: 4284-4296.
Filya I. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. J Dairy Sci 2003; 86: 3575-3581.
Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Ng J, Munro K, Alatossava T. Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Appl Envion Microbiol 1999; 65: 4264-4267.
Mortazavian A, Razavi SH, Ehsani MR, Sohrabvandi S. Principles and methods of microencapsulation of probiotic microorganism. A review article. Ir J Biotech 2007; 5: 1-18.
Anal AK, Singh H. Recent advances in microencapsula- tion of probiotics for industrial applications and targeted delivery. Trends Food Sci Technol 2007; 18: 240-251.
Kailasapathy K. Microencapsulation of probiotic bacteria: Technology and potential applications. Curr Issues Intest Microbiol 2002. 3: 39-48.
No HK, Kim SH, Lee SH, Park NY, Prinyawiwatkul W.Stability and antibacterial activity of chitosan solutions affected by storage temperature and time. Carbohydrate Polymer 2006; 65: 174-178.
Signoretto C, Lleò MM, Tafi MC, Canepari P. Cell wall chemical composition of Enterococcus faecalis in the viable but nonculturable state. Appl Environ Microbiol 2003. 66: 1953-1959.
CAI Y. Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. J Dairy Sci 1999;82: 2466-2471.
Mir-hoseini M. Study of effect of nisin and producer bacteria of nisin on Listeria monocytogenes and Bacillus cereus.Master thesis. Univ of Isfahan Iran 2004; 42-45.
Lleó MM, Tafi MC, Canepari P. Nonculturable Enterococcus faecalis cells are metabolically active and capable of resuming active growth. Syst Appl Microbiol 1998; 21: 333-339.
Chou LS, Weimer B. Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J Dairy Sci 1999; 82: 23-31.
Succi M, Tremonte P, Reale A, Sorrentino E, Grazia L, Pacifico S, et al. Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. FEMS Microbiol Lett 2005; 244: 129-137.
Lee JS, Cha DS, Park HJ. Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles. J Agric Food Chem 2004; 52: 7300-7305.
Krasaekoopt W, Bhandari B, Deeth HC. Survival of probiotics encapsulated in chitosan-coated alginate beads in yoghurt from UHT- and conventionally treated milk during storage. Food Sci Technol 2006; 39: 177-183.
Klinkenberg G, KQ, Levine DW, Dyrset N. Cell release from alginate immobilized Lactococcus lactis ssp. Lactis in chitosan and alginate coated beads. J Dairy Sci 2001; 84: 1118-1127.
Collins DM, Gibson G. Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Inter Dairy J 1999. 69: 10525-10575.
Santoso B, Mwenya B, Sar C, Gamo Y, Kobayashi T, Morikawa R, et al. Effects of supplementing galacto- oligosaccharides, Yucca schidigera or nisin on rumen methanogenesis, nitrogen and energy metabolism in sheep. Livestock Production. J Anim Sci 2003; 91: 209-217.
Xing L, Chen LJ, Han LJ. The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Biosource Technol 2008; 100: 488-491.
Koc F, Ozduven ML. The effect of bacteria+enzyme mixture silage inoculant on the fermentation characteristic, cell wall contents and aerobic stabilities of maize silage. Pakistan J Nutr 2008; 7: 222-226.
Pitt R E. A model of cellulase and amylase additives in silage. J Dairy Sci 1990; 73: 1788-1799.
Cebeci A, Guracan C. Properties of potential probiotic Lactobacillus plantarum strains. Food Microbial 2002;20: 511-518.
Reid G. The scientific basis for probiotic strains of Lacto- bacillus. Appl Envion Microbiol 1999; 65: 3763-3766.
Charteris WP. Ingredient selection criteria for probiotic microorganisms in functional dairy food. Dairy Technol 1998; 51: 123-136.
Andersson HNG, Asp A, Bruce S, Roos T, Wadstro M, Wold A. Health effects of probiotics and prebiotics. A literature review on human studies. Scan J Nutr 2001; 4 kpo; i: 58-75.
Marteau P, Boutron-Ruault MC. Nutritional advantages of probiotics and prebiotics. Br J Nutr 2002; 87: S153-S157.
Makras L, Asker GV, Vuyst LD. Lactobacillus para- casei subsp. paracasei 8700: 2 degrades inulin-type. fructans exhibiting different degrees of polymerization. Am Soc Microbiol 2005; 71: 6531-6537.
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Issue | Vol 4 No 4 (2012) | |
Section | Articles | |
Keywords | ||
Enzyme LAB inulin microencapsulation silage |
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