Cane molasses as a source of precursors in the bioproduction of tryptophan by Bacillus subtilis
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Abstract
Background and Objectives: The essential amino acid L-tryptophan can be produced by a condensation reaction between indole and L-serine, catalyzed by B. subtilis with tryptophan synthase activity. Application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier.
Materials and Methods: The optimum concentration of the Iranian cane molasses was determined by measuring the amount of biomass after growth in 1 to 30 g/mL of molasses. The maximum amount of biomass was obtained in 10 g/mL molasses. Chromatographic methods, TLC and HPLC, were used to assay the amount of tryptophan produced in the presence of precursors of tryptophan production (indole and serine) and/or molasses.
Results: Our results indicate the importance of the Iranian cane molasses not only as carbon source, but also as a source of precursors for tryptophan production.
Conclusion: This report evaluates the potential of cane molasses as an economical source for tryptophan production by B. subtilis, hence eliminating the requirement for additional serine and indole as precursors.
Ruddick JP, Evans AK, Nutt DJ, Lightman SL, Rook GA, Lowry CA. Tryptophan metabolism in the central nervous system: medical implications. Expert Rev Mol Med 2006; 8: 1-27.
Aerts RJ, Verpoorte R. The influence of tryptophan and tryptamine feeding and light on alkaloid biosynthesis in cinchona seedlings. Planta Med 1992; 58: 150-152.3. Millar DB. Negative cooperativity in tryptophan synthase alpha subunit dissociation is caused by the bound coenzyme: Pyridoxal 5′-phosphate. Anal Biochem 1998; 264: 271-278.
Porter RJ MR, Joyce PR, Luty SE. Tryptophan and tyrosine availability and response to antidepressant treatment in major depression. J Affect Disord 2005; 86:129-134.
van Veen JF, van Vliet IM, de Rijk RH, van Pelt J, Mertens B, Fekkes D, et al. Tryptophan depletion affects the autonomic stress response in generalized social anxiety disorder. Psychoneuroendocrinology 2009; 34:1590-1594.
Aune TM, Pogue SL. Inhibition of tumor cell growth by interferon-gamma is mediated by two distinct mechanisms dependent upon oxygen tension: induction of tryptophan degradation and depletion of intracellular nicotinamide adenine dinucleotide. J Clin Invest 1989;84: 863-875.
Riemann D, Feige B, Hornyak M, Koch S, Hohagen F, Voderholzer U. The tryptophan depletion test: impact on sleep in primary insomnia - a pilot study. Psychiatry Res 2002; 109: 129-135.
Beretich GR. Do high leucine/low tryptophan dieting foods (yogurt, gelatin) with niacin supplementation cause neuropsychiatric symptoms (depression) but not dermatological symptoms of pellagra? Medical Hypotheses 2005; 65: 628-629.
Kalyanasundaram S, Ramanamurthy PS. Utilization of tyrosine and tryptophan for protein synthesis by undernourished developing rat brain. Neurochem Res 1983; 8: 1471-1480.
Hamilton BK, Hsiao HY, Swann WE, Anderson DM, Delente JJ. Manufacture of image -amino acids with bioreactors. Trends Biotechnol 1985; 3: 64-68.
Hoch SO. Tryptophan synthetase from Bacillus subtilis purification and characterization of the beta2 component. J Biol Chem 1973; 248: 2999-3003.
Bandiera M, Morpurgo G, Ricci R. Tryptophan production by mutant strains of Escherichia coli K12. Experientia 1967; 23: 724-725.
Gollnick P, Babitzke P, Antson A, Yanofsky C.Regulation of sigL expression by the catabolite control protein CcpA involves a roadblock mechanism in Bacillus subtilis: potential connection between carbon and nitrogen metabolism. Annu Rev Genet 2005; 39:47-68.
Shiio I, Miyajima R, Nakagawa M. Regulation of aromstic amino acid biosynthesis in brevibacterium flavum. J Biochem 1972; 72: 1447-1455.19. Pasupuleti M, Chalupka A, Morgelin M, Schmidtchen A, Malmsten M. Tryptophan end-tagging of antimicrobial peptides for increased potency against Pseudomonas aeruginosa. Biochim Biophys Acta 2009; 1790: 800-808.20. Mateus DM, Alves SS, Da Fonseca MM. Kinetics of L-tryptophan production from indole and L-serine catalyzed by whole cells with tryptophanase activity. J Biosci Bioeng 2004; 97: 289-293.21. Hermann T. Industrial production of amino acids by coryneform bacteria. J Biotechnol 2003; 104: 155-172.
Dehghan Shasaltaneh M, Fooladi J, Moosavi-Nejad SZ. Tryptophan production by Escherichia coli in the presence of Iranian cane molasses. J Paramed Sci (JPS)2010; 1: 19-25.
Degterev EV, Degtyar VG, Vaisburg AF, Krasikov VD, Krylov VM, Malakhova II, etal. Quantitative determination of L-tryptophan by chromatodensitometry on plates. Pharm Chem J 1994; 28: 274-277.
Culley WJ. A rapid and simple thin-layer chromato- graphic method for amino acids in blood. clin chem 1969; 15: 902-907.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 350-356.
Newton WA, Snell EE. An inducible tryptophan synthetase in tryptophan auxotrophs of Escherichia coli. Proc Natl Acad Sci U S A 1962; 48: 1431-1439.
Watanabe J, Fukumoto K, Fukushi E, Sonoyama K, Kawabata J. Isolation of tryptophan as an inhibitor of ovalbumin permeation and analysis of its suppressive effect on oral sensitization. Biosci Biotechnol Biochem 2004; 68: 59-65.
Nakajima N, Tanizawa K, Tanaka H, Soda K.Enantioselective synthesis of various D-amino acids by a multi-enzyme system. J Biotechnol 1988; 8: 243-248.
Nabi SA, Khan MA. Selective TLC separation of lysine and threonine in pharmaceutical preparations. Acta Chromatographica 2003; 13: 161-171.
Ren J, Zhao M, Wang J, Cui C, Yang B.Spectrophotometric method for determination of tryptophan in protein hydrolysates. Food Technol Biotechnol 2007; 45: 360-366.
Atiyeh H, Duvnjak Z. Production of fructose and ethanol from cane molasses using Saccharomyces cerevisiae ATCC 36858. Acta Biotechnol 2003; 23: 37-48.
Fisher SH, Sonenshein AL. Control of carbon and nitrogen metabolism in Bacillus subtilis. Annu Rev Microbiol 1991; 45: 107-35.
Heilmann HD. On the mechanism of action of Escherichia coli tryptophan synthase. Steady-state investigations. Biochim Biophys Acta 1978; 522: 614-624.
Hoch SO. Tryptophan synthetase from Bacillus subtilis.Purification and characterization of the beta2 component.J Biol Chem 1973; 248: 2892-8.
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| Issue | Vol 5 No 3 (2013) | |
| Section | Articles | |
| Keywords | ||
| Bacillus subtilis Tryptophan cane molasses indole mtryptophan synthase | ||
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