Moleculartyping and antibiotic resistance pattern of Aeromonas hydrophila isolated from children with diarrhea in Qom, Iran
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Abstract
Background and Objectives: Today there are reports of the spread of multiple antibiotic resistance in Aeromonas spp., which can be opportunistic pathogens and can cause infection in the host. This study aimed to investigate ERIC-PCR and BOX-PCR typing, drug resistance pattern, and tetracycline, sulfonamide, and fluoroquinolone resistance genes in Aeromonas hydrophila collected from diarrhea samples of children in Qom City, Iran.
Materials and Methods: In our cross-sectional descriptive study, isolates of A. hydrophila isolated from children's diarrhea samples at Hazrat Masoumeh Hospital in Qom were evaluated between 2018 and 2020. Once isolates confirmed, the level of antimicrobial resistance of the strains was determined by the disk diffusion assay following the recommendations of Clinical and Laboratory Standards Institute (CLSI). The genes of tetA, tetB, tetC, tetD, tetE, sul1, sul2, Edq1qc, qnrA, qnrB, qnrS were studied using the PCR method. Then the genetic diversity of the isolates was investigated by ERIC-PCR and box-PCR. Finally, data analysis was done with SPSS version 16.
Results: From 100 A. hydrophila strains, the highest resistance, and sensitivity were related to ampicillin (92%) and azithromycin (96%), respectively. The frequency of tetA, tetB, tetC, tetD, tetE, sul1, sul2, Edq1qc, qnrA, qnrB, qnrS genes was 23, 24, 27, 23, 21, 26, 10, 22, 8, 11, 15%, respectively. With ERIC-PCR and BOX-PCR techniques, all isolates could be typed, so that 8 genetic patterns were determined in ERIC-PCR and 10 genetic patterns were determined in BOX-PCR. A significant relationship was found between cefotaxime and tetA (p = 0.012), ciprofloxacin and tetC (p = 0.018), cefotaxime and qnrA (p = 0.04), and also between tetracycline resistance genes and both sulfonamide genes (p > 0.05).
Conclusion: For the first time this study revealed resistance genes for tetracycline, sulfonamide, and fluoroquinolones in A. hydrophila isolated from childeren’s diarrhea samples in Qom. Even though these antimicrobial resistance genes are present in our study and bacteria are resistant to different types of antimicrobials, it can be predicted that the abundance of these genes will increase in the future. We also observed that A. hydrophila isolates had high genetic diversity.
2. Citterio B, Biavasco F. Aeromonas hydrophila virulence. Virulence 2015; 6: 417-418.
3. Janda JM, Abbott SL. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 2010; 23: 35-73.
4. Soltan Dallal MM, Mazaheri Nezhad Fard R, Kavan Talkhabi M, Aghaiyan L, Salehipour Z. Prevalence, virulence and antimicrobial resistance patterns of Aeromonas spp. isolated from children with diarrhea. Germs 2016; 6: 91-96.
5. Moradi F, Hadi N, Bazargani A, Abdi F, Ghorbanian N. Aeromonas characteristics in Iran, Southwest Asia; a systematic review and meta-analysis on epidemiology, reservoirs and antibiotic resistance profile from aquatic environments to human society during 2000–2023. BMC Vet Res 2025; 21: 107.
6. Sadeghi H, Aslanimehr M, Nikkhahi F, Safari R, Vafaie M, Gholamzadeh Khoei S. A Systematic Review and Meta-Analysis of Aeromonas-Associated Diarrhea Among Children in Asia. Surgical Infect (Larchmt) 2024; 25: 538-545.
7. Stratev D, Odeyemi O. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. J Infect Public Health 2016; 9: 535-544.
8. Kim SR, Nonaka L, Suzuki S. Occurrence of tetracycline resistance genes tet (M) and tet (S) in bacteria from marine aquaculture sites. FEMS Microbiol Lett 2004; 237: 147-156.
9. Li Q, Li Z, Wang Y, Chen Y, Sun J, Yang Y, et al. Antimicrobial resistance and transconjugants characteristics of sul3 Positive Escherichia coli isolated from animals in Nanning, Guangxi province. Animals (Basel) 2022; 12: 976.
10. Qiao Y, Shen H, Wan X, Wang L, Shi W, Li H, et al. Drug resistance of Aeromonas hydrophila isolated from allogyogenetic silver crucian carp Carassius auratus gibelio to sulfonamides. Fish Sci 2018; 37: 456-463.
11. Ruiz J. Transferable Mechanisms of Quinolone Resistance from 1998 Onward. Clin Microbiol Rev 2019; 32(4): e00007-19.
12. Gohil N, Panchasara H, Patel S, Singh V (2019). Molecular biology techniques for the identification and genotyping of microorganisms. In: Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore, pp. 203-226.
13. Cheok YY, Puah SM, Chua KH, Tan J. Isolation and molecular identification of Aeromonas species from the tank water of ornamental fishes. Acta Vet Hung 2020; 68: 130-139.
14. Singh V, Chaudhary DK, Mani I, Somvanshi P, Rathore G, Sood N. Genotyping of Aeromonas hydrophila by Box elements. Mikrobiologiia 2010; 79: 370-373.
15. Puah SM, Khor WC, Kee BP, Tan JAMA, Puthucheary SD, Chua KH. Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species. J Med Microbiol 2018; 67: 1271-1278.
16. Dickert H, Machka K, Braveny IJI. The uses and limitations of disc diffusion in the antibiotic sensitivity testing of bacteria. Infection 1981; 9: 18-24.
17. Szczuka E, Kaznowski A. Typing of clinical and environmental Aeromonas sp. strains by random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacterial repetitive intergenic consensus sequence PCR. J Clin Microbiol 2004; 42: 220-228.
18. Versalovic J. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 1994; 5:25-40.
19. Abdollahi Kheirabadi S, Najafipour S, Kafilzadeh F, Abdollahi A, Jafari S, Moravej A. Evaluation of drug resistance pattern of Escherichia coli strains isolated from Fasa Vali-e-Asr hospital patients. J Fasa Univ Med Sci 2013; 2: 273-278.
20. Balcazar J. Bacteriophages as vehicles for antibiotic resistance genes in the environment. PLoS pathog 2014;10(7):e1004219.
21. Kazemi S, Alikhani MY, Arabestani MR, Sedighi I, Rastyani S, Farhadi Kohan H. Prevalence of Aeromonas hydrophila and Yersinia enterocolitica in children with acute diarrhea attending health centers in Hamadan. Avicenna J Clin Med 2016; 22: 338-345.
22. Soltan Dalal MM, Ghalavand Z, Nikmanesh B. Investigation of Infection Rate to Intestinal Pathogens and Aeromonas Species in Medical Center, 2004-2005. J Adv Med Biomed Res 2005; 13: 37-42.
23. Chenia HY. Prevalence and characterization of plasmid-mediated quinolone resistance genes in Aeromonas spp. isolated from South African freshwater fish. Int J Food Microbiol 2016; 231: 26-32.
24. Kosikowska U, Stec J, Andrzejczuk S, Mendrycka M, Pietras-Ożga D, Stępień-Pyśniak D, et al. Plasmid-Mediated Fluoroquinolone Resistance Genes in Quinolone-Susceptible Aeromonas spp. Phenotypes Isolated From Recreational Surface Freshwater Reservoir. Front Cell Infect Microbiol 2022; 12: 885360.
25. Wimalasena SHMP, De Silva BCJ, Hossain S, Pathirana HNKS, Heo GJ. Prevalence and characterisation of quinolone resistance genes in Aeromonas spp. isolated from pet turtles in South Korea. J Glob Antimicrob Resist 2017; 11: 34-38.
26. Li L, Ye L, Zhang S, Meng H. Isolation and identification of aerobic bacteria carrying tetracycline and sulfonamide resistance genes obtained from a meat processing plant. J Food Sci 2016; 81(6): M1480-M4.
27. Tekedar HC, Arick MA, Hsu C-Y, Thrash A, Blom J, Lawrence ML, et al. Identification of antimicrobial resistance determinants in Aeromonas veronii strain MS-17-88 recovered from channel catfish (Ictalurus punctatus). Front Cell Infect Microbiol 2020; 10: 348.
28. Renson A, Herd P, Dowd JB. Sick individuals and sick (microbial) populations: challenges in epidemiology and the microbiome. Annu Rev Publ Health 2020; 41: 63-80.
29. Schürch A, Arredondo-Alonso S, Willems R, Goering R. Whole genome sequencing options for bacterial strain typing and epidemiologic analysis based on single nucleotide polymorphism versus gene-by-gene–based approaches. Clin Microbiol Infect 2018; 24: 350-354.
30. Almasian-Tehrani N, Alebouyeh M, Armin S, Soleimani N, Azimi L, Shaker-Darabad R, et al. Overview of typing techniques as molecular epidemiology tools for bacterial characterization. Cell Mol Biomed Rep 2021; 1: 69-77.
31. Sharma A, Lee S, Park YS. Molecular typing tools for identifying and characterizing lactic acid bacteria: a review. Food Sci Biotechnol 2020; 29: 1301-1318.
32. Ning K, Zhou R, Li M. Antimicrobial resistance and molecular typing of Staphylococcus aureus isolates from raw milk in Hunan Province. Peer J 2023;11: e15847.
33. Bilung LM, Pui CF, Su’ut L, Apun K. Evaluation of BOX-PCR and ERIC-PCR as molecular typing tools for pathogenic Leptospira. Dis Markers 2018; 2018: 135643.
34. Ahamdi F, Siasi Torbati E, Amini K. A Comparative Study of ERIC-PCR and BOX-PCR Methods for Evaluation of Genomic Polymorphism among Multidrug-Resistant Enterococcus faecium Clinical Isolates. Med Lab J 2023; 17: 13-19.
35. Sepahvand S, Darvishi M, Mokhtari M, Ali Davarpanah M. Evaluation of genetic diversity of colistin-resistant Acinetobacter baumannii by BOX-PCR and ERIC-PCR: the first report. Future Microbiol 2022;1 7: 917-930.
36. Bilung LM, Pui CF, Su’ut L, Apun K. Evaluation of BOX‐PCR and ERIC‐PCR as molecular typing tools for pathogenic Leptospira. Dis Markers 2018; 2018: 1351634.
37. Maiti B, Raghunath P, Karunasagar I, Karunasagar I. Typing of clinical and environmental strains of Aeromonas spp. using two PCR based methods and whole cell protein analysis. J Microbiol Methods 2009; 78: 312-318.
| Files | ||
| Issue | Vol 18 No 3 (2026) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v18i3.21655 | |
| Keywords | ||
| Aeromonas hydrophila Antibiotic sensitivity pattern Polymerase chain reaction (PCR) Molecular typing | ||
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