Chromosome-borne blaCTX-M-65 gene in non-O1/O139 Vibrio cholerae isolated from bile
Abstract
Background and Objectives: Non-O1/O139 Vibrio cholerae (NOVC) has been associated with extraintestinal infections; however, its isolation from bile remains exceedingly rare. This study reports the identification and comprehensive analysis of a NOVC strain isolated from bile, including its antimicrobial resistance profile, virulence gene content, and molecular characteristics.
Materials and Methods: A NOVC isolate was obtained from the bile of a patient with a hepatobiliary tumor. The isolate was identified and subjected to antimicrobial susceptibility testing. Whole-genome sequencing was performed to characterize its molecular features, including antimicrobial resistance genes, virulence genes, and relevant genetic mutations.
Results: The NOVC isolate presents a multi-drug resistance phenotype. Corresponding genomic analysis indicates that this strain belongs to a novel sequence type (ST1736), carrying various drug resistance genes and virulence factors. Moreover, the blaCTX-M-65 extended-spectrum β-lactamase (ESBL) gene was detected in its chromosomal genome.
Conclusion: This study presents the first report of a multidrug-resistant NOVC strain isolated from bile in mainland China. Notably, the ESBL gene blaCTX-M-65 was identified chromosomally in NOVC for the first time.
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| Files | ||
| Issue | Vol 18 No 1 (2026) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v18i1.20906 | |
| Keywords | ||
| Vibrio cholerae Bile Sequence analysis DNA Drug resistance Virulence factors | ||
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