Anti-candida activity of Lacticaseibacillus rhamnosus R-2002 and its possible application in candidiasis prevention
-
Aleksanyan Tigran
,
Hakobyan Lusine
,
Harutyunyan Ruzanna
,
Semerjyan Inesa
,
Nahapetyan Nelli
,
Gharibyan Edita
,
Haertlé Thomas
,
Bazukyan Inga
Abstract
Background and Objectives: Probiotics are used for the treatment of yeast infections, they restore the balance in vaginal microbiome, adhere to epithelial cells, compete against pathogenic bacteria, acidify the environment, produce bacteriocins and modulate the immunity. The aim of the study was to investigate the anti-yeast activity (AYA) of the strain Lacticaseibacillus rhamnosus R-2002 against different Candida species.
Materials and Methods: From 20 strains of lactic acid bacteria examined, only L. rhamnosus R-2002 strain demonstrated beneficial properties against yeast. The effects of temperature and pH on AYA and its relation to cell wall were revealed by bi-layer agar assay. The connection of AYA to the cell wall was determined with the sonicated cells.
Results: R-2002 inhibited the growth of C. albicans ATCC 10291, C. tropicalis G 31 and C. albicans G4 (both isolated from vaginal samples). R-2002 maintained its AYA between a wide range of pH and its anti-yeast component/s are extracellular. The tested strain demonstrated stability against the high concentrations of progesterone and metronidazole, making it a suitable candidate for the mitigation of vaginitis.
Conclusion: The present study summarizes all the positive features of the strain R-2002 and its potential as a therapeutic agent in the treatment of candidiasis.
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26. Sovijit WN, Sovijit WE, Pu S, Usuda K, Inoue R, Watanabe G. et al. Ovarian progesterone suppresses depression and anxiety-like behaviors by increasing the Lactobacillus population of gut microbiota in ovariectomized mice. Neurosci Res 2021; 168: 76-82.
27. Liu H, Zhang R, Zhang Q, Tian M, Ren X, Wang L, et al. Antifungal Activity of Cell-Free Supernatants from Lactobacillus pentosus 86 against Alternaria gaisen. Horticulturae 2023; 9: 911.
28. Rouse S, Harnett D, Vaughan A, van Sinderen D. Lactic acid bacteria with potential to eliminate fungal spoilage in foods. J Appl Microbiol 2008; 104: 915-923.
29. Schwenninger SM, von Ah U, Niederer B, Teuber M, Meile L. Detection of Antifungal Properties in Lactobacillus paracasei subsp. paracasei SM20, SM29, and SM63 and Molecular Typing of the Strains. J Food Prot 2005; 68: 111-119.
30. Liang Q, Zhou W, Peng S, Liang Z, Liu Z, Zhu Ch, et al. Current status and potential of bacteriocin-producing lactic acid bacteria applied in the food industry. Curr Res Food Sci 2025; 10: 100997.
31. De Muynck C, Leroy AI, De Maeseneire S, Arnaut F, Soetaert W, Vandamme EJ. Potential of selected lactic acid bacteria to produce food compatible antifungal metabolites. Microbiol Res 2004; 159: 339-346.
32. Laref N, Guessas B. Antifungal activity of newly isolates of lactic acid bacteria. Innov. Rom. Food Biotechnol 2013; 13: 80-88.
33. Chen H, Ju H, Wang Y, Du G, Yan X, Cui Y, et al. Antifungal activity and mode of action of lactic acid bacteria isolated from kefir against Penicillium expansum. Food Control 2021; 130: 108274.
34. Jurášková D, Ribeiro SC, Silva CCG. Exopolysaccharides Produced by Lactic acid bacteria: from Biosynthesis to Health-Promoting Properties. Foods 2022; 11: 156.
35. Allonsius CN, van den Broek MFL, De Boeck I, Kiekens S, Oerlemans EFM, Kiekens F, et al. Interplay between Lactobacillus rhamnosus GG and Candida and the involvement of exopolysaccharides. Microb Biotechnol 2017; 10: 1753-1763.
2. Bongomin F, Gago S, Oladele RO, Denning DW. Global and multi-National Prevalence of fungal diseases estimate precision. J Fungi (Basel) 2017; 3: 57.
3. Poon Y, Hui M. Inhibitory effect of lactobacilli supernatants on biofilm and filamentation of Candida albicans, Candida tropicalis, and Candida parapsilosis. Front Microbiol 2023; 14: 1105949.
4. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev 2007; 20: 133-163.
5. Pfaller MA, Diekema DJ, Turnidge JD, Castanheira M, Jones RN. Twenty years of the SENTRY antifungal surveillance program: results for Candida species from 1997–2016. Open Forum Infect Dis 2019; 6(Suppl 1): S79-S94.
6. Matsubara VH, Bandara HM, Mayer MP, Samaranayake LP. Probiotics as antifungals in mucosal candidiasis. Clin Infect Dis 2016; 62: 1143-1153.
7. Zheng J, Wittouck S, Salvetti E, Franz CMAP, Harris HMB, Mattarelli P, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int J Syst Evol Microbiol 2020; 70: 2782-2858.
8. Bazukyan I. Identification and comparative characterization of new lactic acid bacteria isolated from Armenian dairy products by phenotypic and molecular methods. Proc YSU B: Chem Biol Sci 2018; 52: 45-51.
9. Movsesyan I, Ahabekyan N, Bazukyan I, Madoyan R, Dalgalarrondo M, Chobert J, et al. Properties and survival under simulated gastrointestinal conditions of lactic acid bacteria isolated from Armenian cheeses and matsuns. Biotechnol Biotechnol Equip 2014; 24: 444-449.
10. Bazukyan I, Matevosyan L, Toplaghaltsyan A, Trchounian A. Antifungal activity of lactobacilli isolated from Armenian dairy products: an effective strain and its probable nature. AMB Express 2018; 8: 87.
11. Toplaghaltsyan A, Bazukyan I, Trchounian A. The effects of different carbon sources on the antifungal activity by lactic acid bacteria. Curr Microbiol 2017; 74: 168-174.
12. Matevosyan L, Bazukyan I, Trchounian A. Antifungal and antibacterial effects of newly created lactic acid bacteria associations depending on cultivation media and duration of cultivation. BMC Microbiol 2019; 19: 102.
13. Adnane M, Chapwanya A. Microbial Gatekeepers of Fertility in the Female Reproductive Microbiome of Cattle. Int J Mol Sci 2024; 25: 10923.
14. Simoes JA, Aroutcheva AA, Shott S, Faro S. Effect of metronidazole on the growth of vaginal lactobacilli in vitro. Infect Dis Obstet Gynecol 2001; 9: 41-45.
15. Ferreira RLPS, Nova BGV, Carmo MS, Abreu AG. Mechanisms of action of Lactobacillus spp. in the treatment of oral candidiasis. Braz J Biol 2024; 84։ e282609.
16. Oliveira VM, Santos SS, Silva CR, Jorge AO, Leão MV. Lactobacillus is able to alter the virulence and the sensitivity profile of Candida albicans․ J Appl Microbiol 2016; 121: 1737-1744.
17. Mailänder-Sánchez D, Braunsdorf C, Grumaz C, Müller C, Lorenz S, Stevens P. et al. Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. PLoS One 2017; 12(10): e0184438.
18. Borges S, Silva J, Teixeira P. The role of lactobacilli and probiotics in maintaining vaginal health. Arch Gynecol Obstet 2014; 289: 479-489.
19. Ribeiro FC, de Barros PP, Rossoni RD, Junqueira JC, Jorge AO. Lactobacillus rhamnosus inhibits Candida albicans virulence factors in vitro and modulates immune system in Galleria mellonella. J Appl Microbiol 2017; 122: 201-211.
20. Tan Y, Leonhard M, Moser D, Ma S, Schneider-Stickler B. Inhibitory effect of probiotic lactobacilli supernatants on single and mixed non-albicans Candida species biofilm. Arch Oral Biol 2018; 85: 40-45.
21. Keryan A, Bazukyan I, Trchounian A. Lactobacilli isolated from the Armenian fermented milk product matsoun: Growth properties, antibacterial and proteolytic activity and their dependence on pH. Int J Dairy Technol 2016; 69: 1-10.
22. Ahmadova A, Todorov SD, Hadji-Sfaxi I, Choiset Y, Rabesona H, Messaoudi S, et al. Antimicrobial and antifungal activities of Lactobacillus curvatus strain isolated from homemade Azerbaijani cheese. Anaerobe 2013; 20: 42-49.
23. Afzali S, Edalatian Dovom MR, Habibi Najafi MB, Mazaheri Tehrani M. Determination of the anti-yeast activity of Lactobacillus spp. isolated from traditional Iranian cheeses in vitro and in yogurt drink (Doogh). Sci Rep 2020; 10: 6291.
24. Bounaix MS, Gabriel V, Morel S, Robert H, Rabier P, Remaud-Siméon M, et al. Biodiversity of Exopolysaccharides Produced from Sucrose by Sourdough Lactic Acid Bacteria. J Agric Food Chem 2009; 57: 10889-10897.
25. Bulgasem BY, Lani MN, Hassan Z, Wan Yusoff WM, Fnaish SG. Antifungal activity of Lactic Acid Bacteria Strains isolated from natural Honey against Pathogenic Candida Species. Mycobiology 2016; 44: 302-309.
26. Sovijit WN, Sovijit WE, Pu S, Usuda K, Inoue R, Watanabe G. et al. Ovarian progesterone suppresses depression and anxiety-like behaviors by increasing the Lactobacillus population of gut microbiota in ovariectomized mice. Neurosci Res 2021; 168: 76-82.
27. Liu H, Zhang R, Zhang Q, Tian M, Ren X, Wang L, et al. Antifungal Activity of Cell-Free Supernatants from Lactobacillus pentosus 86 against Alternaria gaisen. Horticulturae 2023; 9: 911.
28. Rouse S, Harnett D, Vaughan A, van Sinderen D. Lactic acid bacteria with potential to eliminate fungal spoilage in foods. J Appl Microbiol 2008; 104: 915-923.
29. Schwenninger SM, von Ah U, Niederer B, Teuber M, Meile L. Detection of Antifungal Properties in Lactobacillus paracasei subsp. paracasei SM20, SM29, and SM63 and Molecular Typing of the Strains. J Food Prot 2005; 68: 111-119.
30. Liang Q, Zhou W, Peng S, Liang Z, Liu Z, Zhu Ch, et al. Current status and potential of bacteriocin-producing lactic acid bacteria applied in the food industry. Curr Res Food Sci 2025; 10: 100997.
31. De Muynck C, Leroy AI, De Maeseneire S, Arnaut F, Soetaert W, Vandamme EJ. Potential of selected lactic acid bacteria to produce food compatible antifungal metabolites. Microbiol Res 2004; 159: 339-346.
32. Laref N, Guessas B. Antifungal activity of newly isolates of lactic acid bacteria. Innov. Rom. Food Biotechnol 2013; 13: 80-88.
33. Chen H, Ju H, Wang Y, Du G, Yan X, Cui Y, et al. Antifungal activity and mode of action of lactic acid bacteria isolated from kefir against Penicillium expansum. Food Control 2021; 130: 108274.
34. Jurášková D, Ribeiro SC, Silva CCG. Exopolysaccharides Produced by Lactic acid bacteria: from Biosynthesis to Health-Promoting Properties. Foods 2022; 11: 156.
35. Allonsius CN, van den Broek MFL, De Boeck I, Kiekens S, Oerlemans EFM, Kiekens F, et al. Interplay between Lactobacillus rhamnosus GG and Candida and the involvement of exopolysaccharides. Microb Biotechnol 2017; 10: 1753-1763.
| Files | ||
| Issue | Vol 17 No 3 (2025) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v17i3.18827 | |
| Keywords | ||
| Antifungal activity Lacticaseibacillus rhamnosus Candida albicans Candidiasis Bacteriocins | ||
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How to Cite
1.
Tigran A, Lusine H, Ruzanna H, Inesa S, Nelli N, Edita G, Thomas H, Inga B. Anti-candida activity of Lacticaseibacillus rhamnosus R-2002 and its possible application in candidiasis prevention. Iran J Microbiol. 2025;17(3):441-450.
