Serotyping and Cross-Reactivity’s Between Different Pseudomonas aeruginosa Isolates Prevalent in Iran
-
H Ahmadi
,
S Malekian
,
B Tabaraie
,
D Norouzian
,
F Poormirza-gholi
,
M Nejati
,
MH Hedayati
,
MR Beik Mohammadi
,
A Behnoodi
,
M Izadpanahi
Abstract
Background and Objectives: 300 Pseudomonas aeruginosa strains were isolated from hospitalized patients in Iran. Using international antigenic typing system (IATS) antibodies, all strains were classified into 16 serotypes while serotype 14 was not identified among the 17 known serotypes. To evaluate the rate of cross-reactivity between O- antigenic determinants, monospecific polyclonal antibodies were made against whole-killed-cells and live cells of each serotype.
Material and Methods: Each antiserum was challenged against homologous and heterologous antigens using slide agglutination test. The degree of agglutination reaction is shown by -ve, 1+ve, 2+ve, 3+ve and 4ve for 0, 25%, 50%, 75% and 100% agglutination respectively. Then, the results were tabulated for further study.
Results: The rate of cross-reactivity between O-antigenic determinants demonstrated that strains 10.55 and 15.14 had the highest agglutination reaction with serum of all the homologous and heterologous serotypes.
Conclusion: Evaluation of the results obtained from the present study can be applied in production of reliable vaccines and antisera as therapeutic agents or as diagnostic kits.
Kusama H. Serological classification of Pseudomonas aeruginosa by a slide agglutination test. J Clin Microbiol 1978; 8: 181-188.
Brokopp CD, Gomez-Lus R, Farmer JJ 3rd. Serological typing of Pseudomonas aeruginosa: use of commercial antisera and live antigens. J Clin Microbiol 1977; 5: 640-649.
Bouza E, Garcia-Garrote F, Cercenado E, Marin M, Diaz MS. Pseudomonas aeruginosa : a Survey of Resistance in 136 Hospital in Spain. J Antimicrob Chemother 1999; 43:981-982.
Penner JL, Whiteley R. Cross-protection of mice provided by active and passive immunization against experimental infection with virulent Proteus rettgeri and Providencia bacteria. Infect Immun 1978; 20: 347-351.
Faure K, Shimabukuro D, Ajayi T, Allmond LR, Sawa T, Wiener-Kronish JP. O-antigen serotype and type III secretory toxins in clinical isolates of Pseudomonas aeruginosa. J Clin Microbiol 2003; 41: 2158-2160.
Masoud H, Sadovskaya I, Kievit T, Altman E, Richards JC, Lam J S. Structural elucidation of the lipopolysaccharide core region of the O-chain-deficient mutant strain A28 from Pseudomonas aeruginosa serotype 06 (International Antigenic Typing Scheme). J Bacteriol 1995; 177: 6718-6726.
Higeta S, Yasunaga Y, Ogata M. Type-specific indirect hemagglutinating antibody in patients with Pseudomonas aeruginosa infection. J Clin Microbiol 1978; 8: 489-495.
Janet AMF, Harris G, Govan RWJ. Revised. Pyocin typing method for Peudomonas aeruginosa. J Clin Microbiol 1984; 20: 47-50.
Head NE, Yu H. Cross-Sectional Analysis of clinical and environmental isolates of Pseudomonas aeruginosa: Bio- film formation, virulence, and genome diversity. Infect Immun 2004; 72: 133-144.
Pier GB. Cross-protection by Pseudomonas aeruginosa polysaccharides. Infect Immun 1982; 38: 1117-1122.
Lényi B. Serological properties of Pseudomonas aeruginosa.I. Group-specific somatic antigens. Acta Microbiol Acad Sci Hung 1966/67; 13: 295-318.
Meitert T. Contribution à l’étude de la structure antigénique des B. pyocyaniques (Pseudomonas aeruginosa). II. Individualisation des groupes sérologiques au moyen des antigènes O. Arch Roum Pathol Exp Microbiol 1964; 23:679-688.
Habs J. Untersuchungen uber die 0-antigene von Pseudomonas aeruginosa. Z Hyg 1957; 114: 218-228.
Homma JY. A new antigenic schema and live-cell slide- agglutination procedure for the intra-sub-specific, serologic classification of Pseudomonas aeruginosa. Jpn J Exp Med 1976; 46: 329-336.
Oldak E, Trafny E. A. Secretion of protease by Pseudomonas aeruginosa bio-films exposed to ciprofloxacin. J Antimicrob Chemother 2005; 49: 3281-3288.
Miyairi SH, Tateda K. Fuse TE, Ueda CH, Saito H, Takabatake T, et al. Immunization with 3-oxododecanoyl- L-homoserine lactone-protein conjugate protects mice from lethal Pseudomonas aeruginosa lung infection. J Med Microbiol 2006; 55: 1381-1387.
Doring G, Pier GB. Vaccines and immunotherapy against Pseudomonas aeruginosa. Vaccine 2008; 26, 1011-1024.
Knirel YA, Bystrova OV, Kocharova NA, Zahringer U, Pier GB. Conserved and variable structural features in the lipopolysaccharide of Pseudomonas aeruginosa. J Endotox Res 2006; 12: 324-336.
Liu PV. Comparison of the Chinese Schema and the International Antigenic Typing System for Serotyping Pseudomonas aeruginosa. J Clin Microbiol 1987; 25:824-826.
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| Issue | Vol 2 No 2 (2010) | |
| Section | Articles | |
| Keywords | ||
| Pseudomonas aeruginosa agglutination test cross-reactivity antigenic schema | ||
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