Genetic evaluation of Locus of enterocyte effacement pathogenicity island (LEE) in Enteropathogenic Escherichia coli isolates (EPEC)
Abstract
Background and Objectives: Enteropathogenic Escherichia coli (EPEC) divided into two groups typical and atypical (aspect). The main virulence genes are located in a pathogenicity island called LEE (Locus of Enterocyte Effacement). LEE frequently inserted in tRNA genes of selC, pheU and pheV in the bacterial chromosome. tEPEC and aEPEC strains have some differences in their pathogenicity. The purpose of this was to investigate the possible differences between tEPEC and aEPEC strains according to the virulence genes encoding by LEE and their relation to insertion sites.
Materials and Methods: In this study 130 E. coli isolates confirmed by biochemical analysis from diarrheal patients, were evaluated for EPEC pathotype by PCR. All EPEC strains tested for presence of some LEE encoded virulence genes and sites of LEE insertion by PCR method.
Results: Among 50 strains of EPEC 28 (56%) and 22 (44%) were typical and atypical strains respectively. 19 strains (30%) showed insertion in selC, 7 (14%) in pheU, 4 (8%) in pheV, 8 (16%) in pheU and pheV, 1 (2%) in selC and pheU, 6 (12%) in pheV, pheU and selC and 5 (10%) had no insertion in these sites. Moreover, spa (n = 8, 16%), espB (n = 16, 32%), espD (n =18, 36%), espF (n = 8, 16%), espG (n = 13, 26%), espH (n = 12, 24%), map (n = 11, 32%) and tir (n = 4, 8%) were present among the strains.
Conclusion: Results showed that most of the virulence genes are present in tEPEC isolates. However, aEPEC isolates may acquire other virulence factors. The majority of tEPEC strains showed insertion at selC and aEPEC strains in pheV and pheU.
Hernandes RT, Elias WP, Vieira MA, Gomes TA. An overview of atypical enteropathogenic Escherichia coli. FEMS Microbiol Lett 2009; 297: 137-149.
Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004; 2: 123-140.
Asadi Karam M, Bouzari S, Oloomi M, Aslani M, Jafari A. Phenotypic and genotypic characterization of enteropathogenic Escherichia coli (EPEC) strains in Tehran, Iran. Iran J Microbiol; 2: 3-7.
Reid SD, Herbelin CJ, Bumbaugh AC, Selander RK, Whittam TS. Parallel evolution of virulence in pathogenic Escherichia coli. Nature 2000; 406 : 64-67.
Trabulsi LR, Keller R, Tardelli Gomes TA. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis 2002; 8: 508-513.
McGraw EA, Li J, Selander RK, Whittam TS. Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli. Mol Biol Evol 1999; 16: 12-22.
Yamamoto D, Hernandes RT, Blanco M, Greune L, Schmidt MA, Carneiro SM, et al. Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia coli strains with different uncommon intimin types. BMC Microbiol 2009; 9: 146.
Rodriguez-Escudero I, Hardwidge PR, Nombela C, Cid VJ, Finlay BB, Molina M. Enteropathogenic Escherichia coli type III effectors alter cytoskeletal function and signalling in Saccharomyces cerevisiae. Microbiology 2005; 151: 2933-2945.
Kenny B. Enteropathogenic Escherichia coli (EPEC)a crafty subversive little bug. Microbiology 2002; 148:1967-1978.
Crepin VF, Martinez E, Shaw RK, Frankel G, Daniell SJ. Structural and functional properties of chimeric EspA-FliC filaments of EPEC. J Mol Biol 2008 18; 378:243-250.
Schmidt H, Hensel M. Pathogenicity islands in bacterial pathogenesis. Clin Microbiol Rev 2004; 17: 14-56.
Mairena EC, Neves BC, Trabulsi LR, Elias WP.Detection of LEE 4 region-encoded genes from different enteropathogenic and enterohemorrhagic Escherichia coli serotypes. Curr Microbiol 2004; 48: 412-418.
Celli J, Deng W, Finlay BB. Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells: exploiting the host cell cytoskeleton from the outside. Cell Microbiol 2000; 2: 1-9.
Rumer L, Jores J, Kirsch P, Cavignac Y, Zehmke K, Wieler LH. Dissemination of pheU- and pheV-located genomic islands among enter pathogenic (EPEC) and enter hemorrhagic (EHEC) E. coli and their possible role in the horizontal transfer of the locus of enterocyte effacement (LEE). Int J Med Microbial 2003; 292:463-475.
Bugarel M, Martin A, Fach P, Beutin L. Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains: a basis for molecular risk assessment of typical and atypical EPEC strains. BMC Microbiol 2001; 11: 142.
Zhang WL, Kohler B, Oswald E, Beutin L, Karch H, Morabito S, et al. Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J Clin Microbiol 2002; 40: 4486-4492.
Toma C, Lu Y, Higa N, Nakasone N, Chinen I, Baschkier A, et al. Multiplex PCR assay for identification of human diarrheagenic Escherichia coli. J Clin Microbiol 2003; 41: 2669-2671.
Kobayashi H, Shimada J, Nakazawa M, Morozumi T, Pohjanvirta T, Pallone S, et al. Prevalence and haracteristics of shiga toxin-producing Escherichia coli from healthy cattle in Japan. Appl Environ Microbiol 2001; 67: 484-489.
Sperandio V, Kaper JB, Bortolini MR, Neves BC, Keller R, Trabulsi LR. Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga- toxin producing Escherichia coli (STEC) serotypes. FEMS Microbiol Lett 1998; 164: 133-139.
Nataro JP, Kaper JB. Diarrheagenic Escherichia coli.Clin Microbiol Rev 1998; 11: 142-201.
Moura RA, Sircili MP, Leomil L, Matte MH, Trabulsi LR, Elias WP, et al. Clonally relationship among atypical enter pathogenic Escherichia coli strains isolated from different animal species and humans. Appl Environ Microbiol 2009; 75 : 7399-7408.
Kim JH, Kim JC, Choo YA, Jang HC, Choi YH, Chung JK, et al. Detection of cytolethal distending toxin and other virulence characteristics of enteropathogenic Escherichia coli isolates from diarrheal patients in Republic of Korea. J Microbiol Biotech 2009; 19 :525-529.
Contreras CA, Ochoa TJ, Ruiz J, Lacher DW, Durand D, DebRoy C, et al. Genetic diversity of locus of enterocyte effacement genes of enter pathogenic Escherichia coli isolated from Peruvian children. J Med Microbiol 2012;61: 1114-1120.
Mairena EC, Neves BC, Trabulsi LR, Elias WP.Detection of LEE 4 region-encoded genes from different enteropathogenic and enter hemorrhagic Escherichia coli serotypes. Curr Microbiol 2004; 48: 412-418.
Gartner JF, Schmidt MA. Comparative analysis of locus of enterocyte effacement pathogenicity islands of atypical enter pathogenic Escherichia coli. Infect Immun 2004; 72: 6722-6728.
Muller D, Benz I, Liebchen A, Gallitz I, Karch H, Schmidt MA. Comparative analysis of the locus of enterocyte effacement and its flanking regions. Infect Immun 2009; 77: 3501-3513.
Vieira MA, Salvador FA, Silva RM, Irino K, Vaz TM, Rockstroh AC, et al. Prevalence and characteristics of the O122 pathogenicity island in typical and atypical enter pathogenic Escherichia coli strains. J Clin Microbiol 2010; 48: 1452-1455.
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| Issue | Vol 5 No 4 (2013) | |
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| EPEC LEE tRNA virulence genes | ||
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