Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains


Background and Objectives: Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.
Materials and Methods: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.
Results and Conclusion: None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

Rupali P, Abraham O, Jesudason M, John T, Zachariah A. Treatment failure in typhoid fever with ciprofloxacin susceptible Salmonella enterica Serotype Typhi Diagn Microbiol Infect Dis 2004, 49: 1-3.

Rowe B, Ward LR, Threlfall EJ. Multidrug-resistant Salmonella Typhi: a worldwide epidemic. Clin Infect Dis 1997; 24: 106-109.

Morita M, Takai N, Terajima J, Watanabe H, Kurokawa M, Sagara H, Ohnishi K, Izumiya H. Plasmid-mediated resistance to cephalosporins in Salmonella enterica serovar Typhi. AntimicrobAgents Chemother 2010; 54:3991-3992.

Irajian G, Ranjbar R, Moghadas A. Detection of extended spectrum beta lactamase producing Salmonella spp. and multidrug resistance pattern. Iran J Pathol 2009; 4:128-132.

Jabeen K, Zafar A, Irfan S, Khan E, Mehraj V, Hasan R. Increase in isolation of extended spectrum beta lactamase producing multidrug resistant non typhoidal Salmonellae in Pakistan. BMC Infect Dis 2010; 10: 101.

Watve MG, Tickoo R, Jog MM, Bhole BD. How many antibiotics are produced by the genus Strepto-myces? Arch Microbiol 2001; 176: 386-390.

Hamedi J, Mohammadipanah F, Klenk HP. Streptomyces iranensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 2010a; 60: 1504-1509.

Hamedi J, Mohammadipanah F, Klenk HP, Pötter G, Schumann P and Kroppensted RM. Nocardiopsis sinuspersici sp. nov., isolated from the sandy rhizospheric soil. Int J Syst Evol Microbiol 2010b; 60: 2346-2352.

Hamedi J, Mohammadipanah F, Pötter G,Spröer method for reconstructing phylogenetic trees. Mol Biol C, Schumann P, Göker M, Klenk HP. Nocardiopsis arvandica sp. nov., isolated from the banks of the Arvand river in Iran. Int J Syst Evol Microbiol 2010c;61: 1466-5026.

Hamedi J, Mohammadipanah F, Maghsoudi N, Khodagholi F, Klenk HP. Inhibition of oxidative stress- induced amyloid β formation in NT2 neurons by culture filtrate of a strain of Streptomyces antibioticus. Appl Microbiol Biotechnol 2010d; 86: 1805-1811.

Hamedi J, Mohammadipanah F, Klenk H, Pötter G, Schumann P, Spröer C and Klenk HP. Nocardiopsis arvandica sp nov, isolated from the sandy soil of Iran. Int J Syst Evol Microbiol 2011; 61: 1189-1194.

Mohammadipanah F, Matasyoh J, Hamedi J, Klenk H P, Laatsch H. Persipeptides A and B, Two Cyclic Peptides from Streptomyces sp. UTMC 1154. Bioorg Medicinal Chem 2012; 20: 335-339.

Chatterjee S, Nadkarni SR, Vijayakumar EKS, Patel MV, Ganguli BN. Napsamycins, new Pseudomonas active antibiotics of the mureidomycin family from Streptomyces sp. HIL Y-82,11372. J Antibiot 1994; 47:595-598.

Saadoun I, Gharaibeh R. The Streptomyces flora of Jordan and it’s potential as a source of antibiotics active against antibiotic-resistant Gram-negative bacteria. World J Microbiol Biotechnol 2002; 18: 465-470.

Galatenko OA, Trekhova LP. Isolation of antibiotic producing actinomycetes from soil samples exposed to UV light. Antibiot Khimioter 1990; 35: 6-8.

EL-Nakeeb MA, Lechevalier HA. Selective isolation of aerobic actinomycetes. Appl Microbiol 1963; 11: 75-77.

Lorian V. (2005) Antibiotics in laboratory medicine.Lippincott Williams and Wilkins, Baltimore, pp 1-90.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.

Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313-340.

Bergey DH, Williams ST, Sharpe ME, Holt JG. (1989) Bergey’s manual of systematic bacteriology. Lippincott Williams and Wilkins, Baltimore, pp 2452-2492.

Rabah FL, Elshafei A, Saker M, Cheikh B, Hocine H.Screening, isolation and characterization of a novel antimicrobial producing actinomycete, strain RAF10. Asian J Biotechnol 2007; 6: 489-495.

Boudjella H, Bouti K, Zitouni A, Mathieu F, Lebrihi A, Sabaou N. Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiol Res 2006; 161: 288-298.

Kawasaki H, Koyama K, Kurokawa S, Watanabe K, Nakazawa M, Izawa K, Nakamatsu T. Production of (R)-3-amino-3-phenylpropionic acid and (S)-3-amino-3-phenylpropionic acid from (R,S)-N-acetyl-3-amino-3-phenylpropionic acid using microorganisms having enantiomer-specific amidohydrolyzing activity. Biosci Biotechnol Biochem 2006; 70: 99-106.

Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406-425.

Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ.Cancer statistics. CA Cancer J Clin 2007; 57: 43-66.

Ramakrishnan J, Shunmugasundaram M, Narayanan M.

Streptomyces sp. SCBT isolated from rhizosphere soil of medicinal plants is antagonistic to pathogenic bacteria. Iran J Biotechnol 2009; 7: 75-81.

Arunachalam R, Wesely EG, George J, Annadurai G.Novel approaches for identification of Streptomyces noboritoensis TBG-V20 with cellulase production. Curr Res Bacteriol 2010; 3: 15-26.

Rong X, Huang Y. Taxonomic evaluation of the Streptomyces griseus clade using multilocus sequence analysis and DNA–DNA hybridization, with proposal to combine 29 species and three subspecies as 11 genomic species. Int J Syst Evol Microbiol 2010; 60: 696-703.

Zhao XQ, Li WJ, Jiao WC, Li Y.Streptomyces xinghaiensis sp. nov., isolated from marine sediment.Int J Syst Evol Microbiol 2009; 59: 2870-2874.

Lacey J. Nomenclature of Saccharopolyspora erythraea Labeda and Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948, and proposals for the alternative epithet Streptomyces labedae sp. nov. Int J Syst Bacteriol 1987; 37: 458.

Palleroni NJ, Reichelt KE, Mueller D, Epps R, Tabenkin B. Production of a novel red pigment, rubrolone, by Streptomyces echinoruber sp. nov. J Antibiot (Tokyo).1978; 31: 1218-1225.

Kuster E. Simple working key for the classification and identification of named taxa included in the international Streptomyces project. J Syst Bacteriol 1972; 22: 139-148.

Luo Y, Xiao J, Wang Y, Xu J, Xie S, Xu J. Streptomyces indicus sp. nov., a novel actinomycete isolated from deep-sea sediment of the Indian Ocean. Int J Syst Evol Microbiol 2010; 61: 2712-2716.

Shirling E B, Gottlieb D. Cooperative description of type cultures of Streptomyces. V. Additional descriptions. Int J Syst Bacteriol 1972; 22: 265-394.

Wu RY, Chen MH. Identification of the Streptomyces strain KS3-5. Bot Bull Acad Sin 1995; 36: 201-205.

Waites MJ, Morgan, NL, Rockey JS, Higton G. (2001) Industrial Microbiology: An Introduction, Wiley-Blackwell, pp 75-85.

Baskaran R, Vijayakumar R, Mohan PM. Enrichment method for the isolation of bioactive actinomycetes from mangrove sediments of Andaman Islands, India.Malaysia J Microbiol 2011; 7: 26-32.

Hozzein WN, Rabie W, Ali MIA. Screening the Egyptian desert actinomycetes as candidates for new antimicrobial compounds and identification of a new desert Streptomyces strain. Afr J Biotechnol 2011; 10:2295-2301.

Remya M, Vijayakumar R. Isolation and characterization of marine antagonistic actinomycetes from west coast of India. Facta Univ Ser Med Biol 2008; 15: 13-19.

Satheeja SV, Jebakumar SRD. Phylogenetic analysis and antimicrobial activities of Streptomyces isolates from mangrove sediment. J Basic Microbiol 2011; 51:71-79.

Hayakawa Y, Iwakiri T, Imamura K, Seto H, Otake N. Studies on the isotetracenone antibiotics. III. A new isotetracenone antibiotic, grincamycin. J Antibiot (Tokyo) 1987; 40: 1785-1787.

Sajid I, Shaaban KA, Hasnain S. Antitumour compounds from a saline soil isolate, Streptomyces griseoincarnatus CTF15. Nat Prod Res 2011; 25: 549-559.

Sui X, Yin J, Ren X. Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies. Antivir Res 2010; 85: 346-353.

IssueVol 5 No 4 (2013) QRcode
Actinomycetes Antibiotic Cytotoxic activity Salmonella enterica serovar Typhi

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Goljanian Tabrizi S, Hamedi J, Mohammadipanah F. Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains. Iran J Microbiol. 1;5(4):356-365.