Heterogeneity of Iranian clinical isolates of Mycobacterium fortuitum
-
Abdolrazagh Hashemi-Shahraki
,
Parvin Heidarieh
,
Maryam Biranvand
,
Saeed Zaker Bostanabad
,
Nasrin Sheikhi
,
Mohamad Hashemzadeh
,
Masume Karami
,
Mohammad Mehdi Feizabadi
Abstract
Background and Objectives: The increase of infections caused by nontuberculous mycobacteria (NTM) is receiving increasing attention worldwide. Mycobacterium fortuitum is encountered with increasing frequency in clinical laboratories of Iran.
Materials and Methods: Sequence variation of 48 M. fortuitum clinical isolates, were investigated by sequence analysis of the 16S-23S Internal Transcribed Spacer.
Results: Twelve different sequence types (sequevar) were identified by sequence analysis of ITS region. Seven previously described sequevar including MfoA, MfoB, MfoC, MfoD, MfoE, MfoF and MfoG identified. Five novel sequevar namely MfoH, MfoI, MfoJ, MfoK and MfoL that were distinctly different from the previously described sequevar were detected among different clinical strains of M. fortuitum, from Iran.
Conclusion: This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level. The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran. The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.
Katoch VM. Infections due to non-tuberculous mycobacteria (NTM). Indian J Med Res 2004; 120:290-304.
Patel JB, Leonard DG, Pan X, Musser JM, Berman RE, Nachamkin I. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system. J Clin Microbiol 2000; 38: 246-251.
Lebrun L, Espinasse F, Poveda JD, Vincent-Levy- Frebault V. Evaluation of nonradioactive DNA probes for identification of mycobacteria. J Clin Microbiol 1992; 30: 2476-2478.
De Beenhouwer H, Liang Z, De Rijk P, Van Eekeren C, Portaels F. Detection and identification of mycobacteria by DNA amplification and oligonucleotide-specific capture plate hybridization. J Clin Microbiol 1995; 33:2994-2998.
Tortoli E, Nanetti A, Piersimoni C, Cichero P, Farina C, Mucignat G, Scarparo C, et al. Performance assessment of new multiplex probe assay for identification of mycobacteria. J Clin Microbiol 2001; 39: 1079-1084.
Kim H, Kim SH, Shim TS, Kim MN, Bai GH, Park YG, Lee SH, et al. PCR restriction fragment length polymorphism analysis (PRA)-algorithm targeting 644 bp Heat Shock Protein 65 (hsp65) gene for differentiation of Mycobacterium spp. J Microbiol Methods 2005; 62: 199-209.
Lee H, Park HJ, Cho SN, Bai GH, Kim SJ. Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene. J Clin Microbiol 2000; 38: 2966-2971.
Rogall T, Wolters J, Flohr T, Böttger EC. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol 1990; 40: 323-330.
Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, et al. Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates. Int J Syst Evol Microbiol 2004; 54: 1653-1667.
Tortoli, E. Impact of genotypic studies on myco- bacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 2003; 16: 319-354.
Roth A, Fischer M, Hamid ME, Michalke S, Ludwig W, Mauch H. Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 1998; 36 :139-147.
Roth A, Reischl U, Streubel A, Naumann L, Kroppenstedt RM, Habicht M, et al. Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases. J Clin Microbiol 2000; 38:1094-1104.
Silcox VA, Good RC, Floyd MM. Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol 1981; 14: 686-691.
Legrand E, Radegonde N, Goh KS, Rastogi N. A pulsed- field gel electrophoresis study of Mycobacterium fortuitum in a Caribbean setting underlines high genetic diversity of the strains and excludes nosocomial outbreaks. Int J Med Microbiol.51-57: 292; 2002.
Sampaio JL,Chimara E,Ferrazoli L,da Silva Telles MA,Del Guercio VM,Jericó ZV,et al.Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post- mammaplasty infections. Clin Microbiol Infect 2006;12: 142-149.
Telenti A, Marchesi F, Balz M, Bally F, Böttger EC, Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993; 31:175-178.
Portaeles F, de Rijk P, Jannes G. The 16S-23S rRNA spacer, a useful tool for taxonomical and epidemiologi- cal studies of the M. chelonae complex. Tubercle Lung Dis 1996; 77: 17-18.
Frothingham R, Wilson KH. Molecular phylogeny of the Mycobacterium avium complex demonstrates clinically meaningful divisions. J Infect Dis 1994; 169:305-312.
Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, Holland SM, et al. An official ATS/ IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007 15; 175: 367-416.
Kent PT, Kubica GP. Public health mycobacterio- logy: a guide for the level III laboratory. Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta, Ga. 1985.
Pitcher D G, Saunders N A, Owen R J. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbio 1989; l8: 151-158.
Leblond-Bourget N, Philippe H, Mangin I, Decaris B.16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny. Int J Syst Bacteriol 1996;46: 102-111.
Jeon YS, Chung H, Park S, Hur I, Lee JH, Chun J. jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 2005 15; 21: 3171-3173.
Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24: 1596-1599.
Pavlik I, Svastova P, Bartl J, Dvorska L, Rychlik I.Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals,humans, and the environment and virulence for poultry.Clin Diagn Lab Immunol 2000; 7: 212-217.
Picardeau M, Prod’Hom G, Raskine L, LePennec MP, Vincent V. Genotypic characterization of five subspecies of Mycobacterium kansasii. J Clin Microbiol 1997;35: 25-32.
Stout JE, Hopkins GW, McDonald JR, Quinn A, Hamilton CD, Reller LB, et al. Association between 16S-23S internal transcribed spacer sequence groups of Mycobacterium avium complex and pulmonary disease. J Clin Microbiol 2008; 46: 2790-2793.
Shojaei H, Heidarieh P, Hashemi A, Feizabadi MM, Daei Naser A. Species identification of neglected nontubercu lous mycobacteria in developing country. Jpn J Infect Dis 2011; 64: 265-271.
| Files | ||
| Issue | Vol 6 No 1 (2014) | |
| Section | Articles | |
| Keywords | ||
| ITS Mycobacterium fortuitum diversity | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
