Effect of collagen hydrogel containing Lavandula officinalis essential oil nanoemulsion in wound healing of infectious burn
-
Hossein Teymouri
,
Mojgan Mohammadimehr
,
Mohammad Ahanjan
,
Somayeh Sheidaei
,
Majid Saeedi
,
Amir Mellati
Abstract
Background and Objectives: The main cause of mortality in burn patients is infection from burns. Drug-resistant bacteria are the main causes of wound infection, so alternative antibiotic therapies hold significant importance. The objective of this study was to examine the impact of a collagen hydrogel that contains a nanoemulsion of Lavandula essential oil on the healing process of infected burn wounds.
Materials and Methods: In this experimental study, 20 rats were randomly divided after applying burns with a 10 mm diameter hot plate and infecting the wounds with multidrug-resistant Pseudomonas aeruginosa into four groups, including a positive control, a negative control, the first experiment (collagen hydrogel), and the second experiment (collagen hydrogel containing Lavandula essential oil nanoemulsion). On the 4th, 11th, and 18th days, tissue samples were taken for pathology studies. The important parameters in burn wound healing with hematoxylin and eosin and Masson's trichrome staining methods were investigated and scored according to Abramov’s method.
Results: Based on the pathology findings, experimental groups 1 and 2 compared to the negative and positive control groups were effective in rat infection wound healing. The hydrogel scaffold in the experimental groups increased fibroblasts and angiogenesis compared to the control groups. Epithelization was noticed only in the hydrogel group containing nanoemulsion.
Conclusion: The study findings suggest that the use of collagen hydrogel with Lavandula essential oil nanoemulsion has potential as a wound dressing. This is because it has the potential to effectively promote healing and act as an antibacterial agent to prevent infections.
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2. Zhu C, Zhao J, Kempe K, Wilson P, Wang J, Velkov T, et al. A Hydrogel-Based Localized release of colistin for antimicrobial treatment of burn wound infection. Macromol Biosci 2017; 17: 10.1002/mabi.201600320.
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4. Boateng J, Catanzano O. Advanced therapeutic dressings for effective wound Healing--A review. J Pharm Sci 2015; 104: 3653-3680.
5. Sahana TG, Rekha PD. Biopolymers: Applications in wound healing and skin tissue engineering. Mol Biol Rep 2018; 45: 2857-2867.
6. Samadi A, Azandeh S, Orazizadeh M, Bayati V, Rafienia M, Karami MA. Fabrication and characterization of Glycerol/Chitosan/Polyvinyl Alcohol-Based transparent hydrogel films loaded with silver nanoparticles for antibacterial wound dressing applications. Adv Biomed Res 2021; 10: 4.
7. Abbasi K, Tavakolizadeh S, Hadi A, Hosseini M, Soufdoost RS, Heboyan A, et al. The wound healing effect of collagen/adipose-derived stem cells (ADSCs) hydrogel: In vivo study. Vet Med Sci 2023; 9: 282-289.
8. Ansari MN, Soliman GA, Rehman NU, Anwer MK. Crisaborole loaded nanoemulsion based chitosan gel: formulation, physicochemical characterization and wound healing studies. Gels 2022; 8: 318.
9. Dolgachev VA, Ciotti S, Liechty E, Levi B, Wang SC, Baker JR, et al. Dermal nanoemulsion treatment reduces burn wound conversion and improves skin healing in a Porcine model of thermal burn injury. J Burn Care Res 2021; 42: 1232-1242.
10. Hamdan S, Pastar I, Drakulich S, Dikici E, Tomic-Canic M, Deo S, et al. Nanotechnology-driven therapeutic interventions in wound healing: potential uses and applications. ACS Cent Sci 2017; 3: 163-175.
11. Zarrintaj P, Moghaddam AS, Manouchehri S, Atoufi Z, Amiri A, Amirkhani MA, et al. Can regenerative medicine and nanotechnology combine to heal wounds? The search for the ideal wound dressing. Nanomedicine (Lond) 2017; 12: 2403-2422.
12. Kačániová M, Vukic M, Vukovic NL, Čmiková N, Verešová A, Schwarzová M, et al. An in-depth study on the chemical composition and biological effects of Pelargonium graveolens essential oil. Foods 2023; 13: 33.
13. Mele E. Electrospinning of essential oils. Polymers (Basel) 2020; 12: 908.
14. Samuelson R, Lobl M, Higgins S, Clarey D, Wysong A. The effects of lavender essential oil on wound healing: A review of the Current evidence. J Altern Complement Med 2020; 26: 680-690.
15. Li W, Lan Y, Guo R, Zhang Y, Xue W, Zhang Y. In vitro and in vivo evaluation of a novel collagen/cellulose nanocrystals scaffold for achieving the sustained release of basic fibroblast growth factor. J Biomater Appl 2015; 29: 882-893.
16. Morteza-Semnani K, Saeedi M, Akbari J, Eghbali M, Babaei A, Hashemi SMH, et al. Development of a novel nanoemulgel formulation containing cumin essential oil as skin permeation enhancer. Drug Deliv Transl Res 2022; 12: 1455-1465.
17. Gatabi ZR, Saeedi M, Morteza-Semnani K, Rahimnia SM, Yazdian-Robati R, Hashemi SMH. Green preparation, characterization, evaluation of anti-melanogenesis effect and in vitro/in vivo safety profile of kojic acid hydrogel as skin lightener formulation. J Biomater Sci Polym Ed 2022; 33: 2270-2291.
18. Gupta D, Tator CH, Shoichet MS. Fast-gelling injectable blend of hyaluronan and methylcellulose for intrathecal, localized delivery to the injured spinal cord. Biomaterials 2006; 27: 2370-2379.
19. Shahrezaee M, Salehi M, Keshtkari S, Oryan A, Kamali A, Shekarchi B. In vitro and in vivo investigation of PLA/PCL scaffold coated with metformin-loaded gelatin nanocarriers in regeneration of critical-sized bone defects. Nanomedicine 2018; 14: 2061-2073.
20. Karami P, Mohajeri P, Yousefi Mashouf R, Karami M, Yaghoobi MH, Dastan D, et al. Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center. Saudi J Biol Sci 2019; 26: 1731-1736.
21. Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 2011; 11: 29.
22. Said A, Naeem N, Siraj S, Khan T, Javed A, Rasheed HM, et al. Mechanisms underlying the wound healing and tissue regeneration properties of Chenopodium album. 3 Biotech 2020; 10: 452.
23. Kambouris AR, Brammer JA, Roussey H, Chen C, Cross AS. A combination of burn wound injury and Pseudomonas infection elicits unique gene expression that enhances bacterial pathogenicity. mBio 2023; 14(6): e0245423.
24. Putra A, Ibrahim S, Muhar AM, Kuntardjo N, Dirja BT, Pasongka Z, et al. Topical gel of mesenchymal stem cells-conditioned medium under TNF-α precondition accelerates wound closure healing in full-thickness skin defect animal model. J Med Life 2022; 15: 214-221.
25. Khalid A, Khan R, Ul-Islam M, Khan T, Wahid F. Bacterial cellulose-zinc oxide nanocomposites as a novel dressing system for burn wounds. Bacterial cellulose-zinc oxide nanocomposites as a novel dressing system for burn wounds. Carbohydr Polym 2017; 164: 214-221.
26. Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJ, Alshahrour A, et al. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen 2007; 15: 80-86.
27. Adal AM, Demissie ZA, Mahmoud SS. Identification, validation and cross-species transferability of novel Lavandula EST-SSRs. Planta 2015; 241: 987-1004.
28. Fadel BA, Elwakil BH, Fawzy EE, Shaaban MM, Olama ZA. Nanoemulsion of lavandula angustifolia essential Oil/Gold Nanoparticles: Antibacterial effect against Multidrug-Resistant Wound-Causing bacteria. Molecules 2023; 28: 6988.
29. Demir D, Toygar I, Soylu E, Aksu AT, Türeyen A, Yıldırım I, et al. The effect of lavandula stoechas on wound healing in an experimental diabetes model. The effect of lavandula stoechas on wound healing in an experimental diabetes model. Cureus 2023; 15(9): e45001.
30. Rusu AG, Niță LE, Roșca I, Croitoriu A, Ghilan A, Mititelu-Tarțău L, et al. Alginate-based hydrogels enriched with lavender essential oil: evaluation of physicochemical properties, antimicrobial activity, and in vivo biocompatibility. Pharmaceutics 2023; 15: 2608.
31. Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential Oils-Present status and future perspectives. Medicines (Basel) 2017; 4: 58.
32. Predoi D, Iconaru SL, Buton N, Badea ML, Marutescu L. Antimicrobial activity of new materials based on lavender and basil essential oils and hydroxyapatite. Nanomaterials (Basel) 2018; 8: 291.
33. Keyhanian A, Mohammadimehr M, Nojoomi F, Naghoosi H, Khomartash MS, Chamanara M. Inhibition of bacterial adhesion and anti-biofilm effects of Bacillus cereus and Serratia nematodiphila biosurfactants against Staphylococcus aureus and Pseudomonas aeruginosa. Iran J Microbiol 2023; 15: 425-432.
34. Mathew-Steiner SS, Roy S, Sen CK. Collagen in Wound Healing. Bioengineering (Basel) 2021; 8: 63.
35. Vasireddy L, Bingle LEH, Davies MS. Antimicrobial activity of essential oils against multidrug-resistant clinical isolates of the Burkholderia cepacia complex. PLoS One 2018; 13(8): e0201835.
36. Chaiyana W, Inthorn J, Somwongin S, Anantaworasakul P, Sopharadee S, Yanpanya P, et al. The fatty acid compositions, irritation properties, and potential applications of teleogryllus mitratus oil in nanoemulsion development. Nanomaterials (Basel) 2024; 14: 184.
37. Fattahi R, Ghanbarzadeh B, Dehghannya J, Hosseini M, Falcone PM. The effect of Macro and Nano-emulsions of cinnamon essential oil on the properties of edible active films. Food Sci Nutr 2020; 8: 6568-6579.
38. Masood N, Ahmed R, Tariq M, Ahmed Z, Masoud MS, Ali I, et al. Silver nanoparticle impregnated chitosan-PEG hydrogel enhances wound healing in diabetes induced rabbits. Int J Pharm 2019; 559: 23-36.
2. Zhu C, Zhao J, Kempe K, Wilson P, Wang J, Velkov T, et al. A Hydrogel-Based Localized release of colistin for antimicrobial treatment of burn wound infection. Macromol Biosci 2017; 17: 10.1002/mabi.201600320.
3. Moeini A, Pedram P, Makvandi P, Malinconico M, Gomez d'Ayala G. Wound healing and antimicrobial effect of active secondary metabolites in chitosan-based wound dressings: A review. Carbohydr Polym 2020; 233: 115839.
4. Boateng J, Catanzano O. Advanced therapeutic dressings for effective wound Healing--A review. J Pharm Sci 2015; 104: 3653-3680.
5. Sahana TG, Rekha PD. Biopolymers: Applications in wound healing and skin tissue engineering. Mol Biol Rep 2018; 45: 2857-2867.
6. Samadi A, Azandeh S, Orazizadeh M, Bayati V, Rafienia M, Karami MA. Fabrication and characterization of Glycerol/Chitosan/Polyvinyl Alcohol-Based transparent hydrogel films loaded with silver nanoparticles for antibacterial wound dressing applications. Adv Biomed Res 2021; 10: 4.
7. Abbasi K, Tavakolizadeh S, Hadi A, Hosseini M, Soufdoost RS, Heboyan A, et al. The wound healing effect of collagen/adipose-derived stem cells (ADSCs) hydrogel: In vivo study. Vet Med Sci 2023; 9: 282-289.
8. Ansari MN, Soliman GA, Rehman NU, Anwer MK. Crisaborole loaded nanoemulsion based chitosan gel: formulation, physicochemical characterization and wound healing studies. Gels 2022; 8: 318.
9. Dolgachev VA, Ciotti S, Liechty E, Levi B, Wang SC, Baker JR, et al. Dermal nanoemulsion treatment reduces burn wound conversion and improves skin healing in a Porcine model of thermal burn injury. J Burn Care Res 2021; 42: 1232-1242.
10. Hamdan S, Pastar I, Drakulich S, Dikici E, Tomic-Canic M, Deo S, et al. Nanotechnology-driven therapeutic interventions in wound healing: potential uses and applications. ACS Cent Sci 2017; 3: 163-175.
11. Zarrintaj P, Moghaddam AS, Manouchehri S, Atoufi Z, Amiri A, Amirkhani MA, et al. Can regenerative medicine and nanotechnology combine to heal wounds? The search for the ideal wound dressing. Nanomedicine (Lond) 2017; 12: 2403-2422.
12. Kačániová M, Vukic M, Vukovic NL, Čmiková N, Verešová A, Schwarzová M, et al. An in-depth study on the chemical composition and biological effects of Pelargonium graveolens essential oil. Foods 2023; 13: 33.
13. Mele E. Electrospinning of essential oils. Polymers (Basel) 2020; 12: 908.
14. Samuelson R, Lobl M, Higgins S, Clarey D, Wysong A. The effects of lavender essential oil on wound healing: A review of the Current evidence. J Altern Complement Med 2020; 26: 680-690.
15. Li W, Lan Y, Guo R, Zhang Y, Xue W, Zhang Y. In vitro and in vivo evaluation of a novel collagen/cellulose nanocrystals scaffold for achieving the sustained release of basic fibroblast growth factor. J Biomater Appl 2015; 29: 882-893.
16. Morteza-Semnani K, Saeedi M, Akbari J, Eghbali M, Babaei A, Hashemi SMH, et al. Development of a novel nanoemulgel formulation containing cumin essential oil as skin permeation enhancer. Drug Deliv Transl Res 2022; 12: 1455-1465.
17. Gatabi ZR, Saeedi M, Morteza-Semnani K, Rahimnia SM, Yazdian-Robati R, Hashemi SMH. Green preparation, characterization, evaluation of anti-melanogenesis effect and in vitro/in vivo safety profile of kojic acid hydrogel as skin lightener formulation. J Biomater Sci Polym Ed 2022; 33: 2270-2291.
18. Gupta D, Tator CH, Shoichet MS. Fast-gelling injectable blend of hyaluronan and methylcellulose for intrathecal, localized delivery to the injured spinal cord. Biomaterials 2006; 27: 2370-2379.
19. Shahrezaee M, Salehi M, Keshtkari S, Oryan A, Kamali A, Shekarchi B. In vitro and in vivo investigation of PLA/PCL scaffold coated with metformin-loaded gelatin nanocarriers in regeneration of critical-sized bone defects. Nanomedicine 2018; 14: 2061-2073.
20. Karami P, Mohajeri P, Yousefi Mashouf R, Karami M, Yaghoobi MH, Dastan D, et al. Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a burn center. Saudi J Biol Sci 2019; 26: 1731-1736.
21. Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 2011; 11: 29.
22. Said A, Naeem N, Siraj S, Khan T, Javed A, Rasheed HM, et al. Mechanisms underlying the wound healing and tissue regeneration properties of Chenopodium album. 3 Biotech 2020; 10: 452.
23. Kambouris AR, Brammer JA, Roussey H, Chen C, Cross AS. A combination of burn wound injury and Pseudomonas infection elicits unique gene expression that enhances bacterial pathogenicity. mBio 2023; 14(6): e0245423.
24. Putra A, Ibrahim S, Muhar AM, Kuntardjo N, Dirja BT, Pasongka Z, et al. Topical gel of mesenchymal stem cells-conditioned medium under TNF-α precondition accelerates wound closure healing in full-thickness skin defect animal model. J Med Life 2022; 15: 214-221.
25. Khalid A, Khan R, Ul-Islam M, Khan T, Wahid F. Bacterial cellulose-zinc oxide nanocomposites as a novel dressing system for burn wounds. Bacterial cellulose-zinc oxide nanocomposites as a novel dressing system for burn wounds. Carbohydr Polym 2017; 164: 214-221.
26. Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJ, Alshahrour A, et al. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen 2007; 15: 80-86.
27. Adal AM, Demissie ZA, Mahmoud SS. Identification, validation and cross-species transferability of novel Lavandula EST-SSRs. Planta 2015; 241: 987-1004.
28. Fadel BA, Elwakil BH, Fawzy EE, Shaaban MM, Olama ZA. Nanoemulsion of lavandula angustifolia essential Oil/Gold Nanoparticles: Antibacterial effect against Multidrug-Resistant Wound-Causing bacteria. Molecules 2023; 28: 6988.
29. Demir D, Toygar I, Soylu E, Aksu AT, Türeyen A, Yıldırım I, et al. The effect of lavandula stoechas on wound healing in an experimental diabetes model. The effect of lavandula stoechas on wound healing in an experimental diabetes model. Cureus 2023; 15(9): e45001.
30. Rusu AG, Niță LE, Roșca I, Croitoriu A, Ghilan A, Mititelu-Tarțău L, et al. Alginate-based hydrogels enriched with lavender essential oil: evaluation of physicochemical properties, antimicrobial activity, and in vivo biocompatibility. Pharmaceutics 2023; 15: 2608.
31. Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential Oils-Present status and future perspectives. Medicines (Basel) 2017; 4: 58.
32. Predoi D, Iconaru SL, Buton N, Badea ML, Marutescu L. Antimicrobial activity of new materials based on lavender and basil essential oils and hydroxyapatite. Nanomaterials (Basel) 2018; 8: 291.
33. Keyhanian A, Mohammadimehr M, Nojoomi F, Naghoosi H, Khomartash MS, Chamanara M. Inhibition of bacterial adhesion and anti-biofilm effects of Bacillus cereus and Serratia nematodiphila biosurfactants against Staphylococcus aureus and Pseudomonas aeruginosa. Iran J Microbiol 2023; 15: 425-432.
34. Mathew-Steiner SS, Roy S, Sen CK. Collagen in Wound Healing. Bioengineering (Basel) 2021; 8: 63.
35. Vasireddy L, Bingle LEH, Davies MS. Antimicrobial activity of essential oils against multidrug-resistant clinical isolates of the Burkholderia cepacia complex. PLoS One 2018; 13(8): e0201835.
36. Chaiyana W, Inthorn J, Somwongin S, Anantaworasakul P, Sopharadee S, Yanpanya P, et al. The fatty acid compositions, irritation properties, and potential applications of teleogryllus mitratus oil in nanoemulsion development. Nanomaterials (Basel) 2024; 14: 184.
37. Fattahi R, Ghanbarzadeh B, Dehghannya J, Hosseini M, Falcone PM. The effect of Macro and Nano-emulsions of cinnamon essential oil on the properties of edible active films. Food Sci Nutr 2020; 8: 6568-6579.
38. Masood N, Ahmed R, Tariq M, Ahmed Z, Masoud MS, Ali I, et al. Silver nanoparticle impregnated chitosan-PEG hydrogel enhances wound healing in diabetes induced rabbits. Int J Pharm 2019; 559: 23-36.
| Files | ||
| Issue | Vol 16 No 3 (2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v16i3.15795 | |
| Keywords | ||
| Lavandula; Wound healing; Collagen; Hydrogels; Burns | ||
| Rights and permissions | |
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Teymouri H, Mohammadimehr M, Ahanjan M, Sheidaei S, Saeedi M, Mellati A. Effect of collagen hydrogel containing Lavandula officinalis essential oil nanoemulsion in wound healing of infectious burn. Iran J Microbiol. 2024;16(3):376-388.
