Original Article

Epidemiological and phylogenetic assessment of human respiratory syncytial virus among pediatric patients presenting acute respiratory infections in Shiraz, Iran during 2015-2016

Abstract

Background and Objectives: The pediatric population worldwide bears a significant morbidity and death burden due to acute respiratory infections (ARIs). Human Orthopneumovirus, sometimes referred to as the Human Respiratory Syncytial Virus (HRSV), is one of the main causes of ARIs in infants. The main goal of this study was to identify the genetic diversity of HRSV strains that were circulating in the Iranian population at a certain time of year.
Materials and Methods: Two hundred youngsters less than 12 years old with acute respiratory infections had samples taken from their throat and pharynx secretions. Then, external and hemi-nested PCR were employed, using specific primers targeting the G gene region to detect HRSV. Subsequently, nine randomly selected positive samples were subjected to sequencing. The results were then compared with reference strains cataloged in GeneBank, and phylogenetic tree was constructed using Chromes and MEGA7.
Results: Out of 200 samples, 34 were identified as containing HRSV. Subgroup A was predominant, accounting for 61.76% of cases, followed by subgroup BA (35.29%) and subgroup B (2.94%). Phylogenetic analysis revealed five samples associated with subtype B and four with genotype A. Genomic analysis showed three samples under the GA2 subgroup and one under GA1 for subtype A, and four samples in subgroup BA and one in GB2 for subtype B.
Conclusion: In this study, subgroup A strains, particularly genotype GA2, exhibited a higher prevalence compared to subgroup B strains during the specific period under investigation, shedding light on the genetic landscape of HRSV in this region.

1. Cui G, Qian Y, Zhu R, Deng J, Zhao L, Sun Y, et al. Emerging human respiratory syncytial virus genotype ON1 found in infants with pneumonia in Beijing, China. Emerg Microbes Infect 2013; 2(4): e22.
2. Langedijk AC, Harding ER, Konya B, Vrancken B, Lebbink RJ, Evers A, et al. A systematic review on global RSV genetic data: Identification of knowledge gaps. Rev Med Virol 2022; 32(3): e2284.
3. Shi T, McAllister DA, O'Brien KL, Simoes EAF, Madhi SA, Gessner BD, et al. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study. Lancet 2017; 390: 946-958.
4. Eshaghi A, Duvvuri VR, Lai R, Nadarajah JT, Li A, Patel SN, et al. Genetic variability of human respiratory syncytial virus A strains circulating in Ontario: a novel genotype with a 72 nucleotide G gene duplication. PLoS One 2012; 7(3): e32807.
5. Trento A, Ábrego L, Rodriguez-Fernandez R, González-Sánchez MI, González-Martínez F, Delfraro A, et al. Conservation of G-protein epitopes in respiratory syncytial virus (group A) despite broad genetic diversity: is antibody selection involved in virus evolution? J Virol 2015; 89: 7776-7785.
6. Muñoz-Escalante JC, Comas-García A, Bernal-Silva S, Robles-Espinoza CD, Gómez-Leal G, Noyola DE. Respiratory syncytial virus A genotype classification based on systematic intergenotypic and intra genotypic sequence analysis. Sci Rep 2019; 9: 20097.
7. Faghihloo E, Yavarian J, Jandaghi NZ, Shadab A, Azad TM. Genotype circulation pattern of human respiratory syncytial virus in Iran. Infect Genet Evol 2014; 22: 130-133.
8. Obando-Pacheco P, Justicia-Grande AJ, Rivero-Calle I, Rodríguez-Tenreiro C, Sly P, Ramilo O, et al. Respiratory syncytial virus seasonality: a global overview. J Infect Dis 2018; 217: 1356-1364.
9. RSV virus expected to add pressure on hospitals in many EU/EEA countries this season. European Centre for Disease Prevention and Control (ECDC). 2022.
10. Turi KN, Wu P, Escobar GJ, Gebretsadik T, Ding T, Walsh EM, et al. Prevalence of infant bronchiolitis‐coded healthcare encounters attributable to RSV. Health Sci Rep 2018; 1(12): e91.
11. Li Y, Wang X, Blau DM, Caballero MT, Feikin DR, Gill CJ, et al. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis. Lancet 2022; 399: 2047-2064.
12. Faghihloo E, Salimi V, Rezaei F, Naseri M, Mamishi S, Mahmoodi M, et al. Genetic diversity in the G protein gene of human respiratory syncytial virus among Iranian children with acute respiratory symptoms. Iran J Pediatr 2011; 21: 58-64.
13. Nicolas De Lamballerie C, Pizzorno A, Dubois J, Padey B, Julien T, Traversier A, et al. Human respiratory syncytial virus-induced immune signature of infection revealed by transcriptome analysis of clinical pediatric nasopharyngeal swab samples. J Infect Dis 2021; 223: 1052-1061.
14. Panatto D, Domnich A, Lai PL, Ogliastro M, Bruzzone B, Galli C, et al. Epidemiology and molecular characteristics of respiratory syncytial virus (RSV) among italian community-dwelling adults, 2021/22 season. BMC Infect Dis 2023; 23: 134.
15. Baek YH, Choi EH, Song MS, Pascua PN, Kwon H-I, Park S-J, et al. Prevalence and genetic characterization of respiratory syncytial virus (RSV) in hospitalized children in Korea. Arch Virol 2012; 157: 1039-1050.
16. Tavakoli F, Izadi A, Yavarian J, Sharifi‐Zarchi A, Salimi V, Mokhtari-Azad T. Determination of genetic characterization and circulation pattern of Respiratory Syncytial Virus (RSV) in children with a respiratory infection, Tehran, Iran, during 2018-2019. Virus Res 2021; 305: 198564.
17. Niya MHK, Jeda AS, Tavakoli A, Sohani H, Tameshkel FS, Panahi M, et al. Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017. Iran J Microbiol 2020; 12: 495-502.
18. Malekshahi SS, Samieipour Y, Rahbarimanesh AA, Izadi A, Ghavami N, Razaghipour S, et al. Genetic characterization of G protein in respiratory syncytial virus ON-1 genotype in Tehran. Future Virol 2020; 15: 725-734.
19. Letafati A, Aghamirmohammadali FS, Rahimi‐Foroushani A, Hasani SA, Mokhtari‐Azad T, Yavarian J. No human respiratory syncytial virus but SARS‐CoV‐2 found in children under 5 years old referred to Children Medical Center in 2021, Tehran, Iran. J Med Virol 2022; 94: 3096-3100.
20. Mohebi L, Karami H, Mirsalehi N, Ardestani NH, Yavarian J, Mard‐Soltani M, et al. A delayed resurgence of respiratory syncytial virus (RSV) during the COVID‐19 pandemic: an unpredictable outbreak in a small proportion of children in the Southwest of Iran, April 2022. J Med Virol 2022; 94: 5802-5807.
21. Zendehrouh M, Karimi A, Azimi L. Respiratory viral infections among children hospitalized in a great Referral Hospital in Iran during the Coronavirus pandemic. Arch Pediatr Infect Dis 2023; 11(3): e136943.
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IssueVol 16 No 3 (2024) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijm.v16i3.15798
Keywords
Respiratory syncytial virus; Seasonal infection; Respiratory tract infections; Genotype; Pediatrics
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Mojarrad S, Tavakoli Movaghar N, Edalat F, Letafati A, Kargar Jahromi Z, Moattari A. Epidemiological and phylogenetic assessment of human respiratory syncytial virus among pediatric patients presenting acute respiratory infections in Shiraz, Iran during 2015-2016. Iran J Microbiol. 2024;16(3):411-420.