Probiotic properties of Lactobacillus strains isolated from gizzard of local poultry
Abstract
Background and Objectives: The aim of our study was to characterize gizzard Lactobacillus fermentum-group strains on the basis of their phenotypic profiles regarding characteristics of lactobacilli. In addition, their in vitro potential probiotic properties were evaluated.
Materials and Methods: The lactic acid bacteria were isolated and identified from gizzard contents of Algerian local poultry using criteria of Bergey's Manual of Determinative Bacteriology and using methods and criteria of Sharpe. The strains were further characterized by tolerance to low pH and bile, coaggregation potential and adhesion to intestinal mucous. The antagonistic activity against some Enterobacteriaceae strains from poultry origin was also evaluated.
Results and Conclusion: Among the strains identified, both physiological and biochemical characteristics differed noticeably. The strains coded LP3 and LP10 survived simulated gastrointestinal conditions and were considered to be acid and bile tolerant. The majority of the strains exhibited antagonistic activity towards Escherichia coli, Klebsiella spp., Enterobacter spp., Shigella spp., Salmonella spp and Citrobacter spp. The best co-aggregation properties were obtained with two isolates. Lb. fermentum LP3 alone showed adherence specificity to the chicken intestinal epithelium.
Turner JL, Dritz, SS, Minton, JE. Review: alternatives to conventional antimicrobials in swine diets. Prof Anim Sci 2001; 17: 217-226.
Fuller R. Probiotics in man and animals. J Appl Bact 1989; 66: 365-378.
Scharek L, Guth J, Reiter K, Weyrauch KD, Taras D, Schwerk P, et al. Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. Vet Imm Immunopath 2005; 105:151-161.
Simpson PJ, Fitagerald GF, Stanton C, Ross RP. The evaluation of a mupirocin-based selective medium for the enumeration of Bifidobacteria from probiotic animal feed. J Microb Meth 2004; 57: 9-16.
Sheehan VM, Sleator RD, Hill C, Fitzgerald GF. Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium brevis UCC2003. Microb 2007; 153:3563-3571.
Shillinger U, Guigas C, Holzapfel WH. In vitro adherence and other properties of lactobacilli used in probiotic yogurt-like products. Int Dairy J 2005; 15:1289-1297.
Giraud E, Brauman A, Keleke S, Leong B, Raimbault M. Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum. Appl Microb Biotech 1991; 36: 379-383.
Sharpe ME (1979). Identification of lactic acid bacteria.In: Identification methods for microbiologists. Skinner, FA and Lovelock, DW. Ed, Academic Press, London. pp. 233-259.
Lin WH, Yu B, Jang SH, Tsen HY. Antimicrobial susceptibility: Different probiotic properties for Lactobacillus fermentum strains isolated from swine and poultry. Anaerobe 2007; 13: 107-113.
Schillinger U, Lucke FK. Antibacterial activity of Lactobacillus sakei isolated from meat. Appl Enviro Microbiol 1989; 55, 1901-1906.
Barnes EM. The intestinal microflora of poultry and game birds during life and after storage. J Appl Bacteriol 1979; 46: 407- 419.
RadaV, Janesova J, Vorisek K, Marounek M.Characteristics of lactobacilli isolated from the chicken intestine. Zentral Mikrob 1991; 16: 227-230.
Fuller R, Barrow PA, Brooker BE. Bacteria associated with the gastric epithelium of neonatal pigs. Appl Enviro Microbiol 1978; 35: 582- 591.
Fons M, Hege T, Ladire M, Raibaud P, Ducluzeau R, Maguin E. Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance. Plasmid 1997; 37: 199-203.
Yu B, Tsen HY. Lactobacillus cells in the rabbit digestive tract and the factors affecting their distribution. J Appl Bacteriol 1993; 75: 269-75.
Idoui T, Leghouchi E, Karam NE. Selection of Lactobacillus plantarum BJ0021 for rabbit probiotic adjuncts. Int J Preb Prob 2007; 2: 188-193.
Ashraf M, Arshad M, Siddique M, Muhammad G. In vitro screening of locally isolated Lactobacillus species for probiotics properties. Pak Vet J 2009; 29: 186-190.
Jin LZ, Ho YM, Abdullah N, Jalaldin S. Acid and bile tolerance of Lactobacillus isolated from chicken. Let Appl Microb1998; 27: 183-185.
Gilliland SE. Beneficial interrelationships between certain microorganisms and human: candidate microorganisms for use as dietary adjuncts. J Food Prot 1979; 42: 164-167.
Patel HM, Pandiella SS, Wang RH, Webb C. Influence of malt, wheat and barley extracts on the bile tolerance of selected strains of lactobacilli. Food Microb 2004;21: 83-89.
Fairbrother JM, Nadeau E, Gyles CL. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Anim Health Res Rev 2005; 6:17-39.
Bhatia SJ, Kochar N, Abraham P.Lactobacillus acidophilus inhibits growth of Campylobacter pylori in vitro. J Cli Microb 1989; 27: 2328-2330.
Rinkinen M, Westermarck E, Salminen S, Ouwehand AC. Absence of host specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus. Vet Microb 2003; 97: 55-61.
Lin W, Hwang C, Chen L, Tsen H. Viable counts, characteristics evaluation for commercial lactic acid bacteria products. Food Microb 2006; 23: 74-81.
Saito T. Selection of useful lactic acid bacteria from Lactobacillus acidophilus group and their applications to functional foods. Anim Sci J 2004; 75: 1-13.
| Files | ||
| Issue | Vol 6 No 2 (2014) | |
| Section | Articles | |
| Keywords | ||
| Gizzard Lactobacillus Local poultry Probiotic | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
