Isolation and characterization of a bisphenol A-degrading strain, Pseudomonas aeruginosa DU2, from soil containing decaying plants
,
Abstract
Background and Objectives: Bisphenol A (BPA) is a toxic compound with broad applications in the plastics industry. BPA has harmful effects on various organisms and its efficient removal is necessary. The microbial degradation of BPA is a safe and economical approach. In this research, soil samples containing decaying plants were screened to isolate a BPA-degradable bacterial strain.
Materials and Methods: Soil samples were collected from different locations in Damghan, Semnan province, Iran. To enrich BPA-degrading bacteria, the samples were cultured in a stepwise manner in a mineral medium containing increasing BPA concentrations (5 to 40 mg/L). The ability of isolated bacteria in degrading BPA was assayed by Folin-Ciocalteu and high-performance liquid chromatography methods. The biodegradation efficiency of the most efficient isolate was assayed under distinct conditions and it was identified through the sequencing of the 16S rRNA gene.
Results: Among the isolated bacteria, Pseudomonas aeruginosa DU2 (GenBank accession number: OP919484) showed the most BPA biodegradation ability. The highest BPA degradation (52.98%) was observed in the mineral medium containing 5 mg/L BPA and the inoculum size of 6 × 107 CFU/mL at pH 9 and in the presence of 0.05% (w/v) NaCl during 10 days.
Conclusion: These results offer soil containing decaying plants as a promising source for finding BPA-degrading bacteria. P. aeruginosa DU2 has basal BPA removal ability, which could be improved by optimization of medium components and growth conditions.
1. Zhang W, Yin K, Chen L. Bacteria-mediated bisphenol A degradation. Appl Microbiol Biotechnol 2013; 97: 5681–5689.
2. Suyamud B, Inthorn D, Panyapinyopol B, Thiravetyan P. Biodegradation of Bisphenol A by a newly isolated Bacillus megaterium strain ISO-2 from a polycarbonate industrial wastewater. Water Air Soil Pollut 2018; 229: 348.
3. Louati I, Dammak M, Nasri R, Belbahri L, Nasri M, Abdelkafi S, et al. Biodegradation and detoxifcation of bisphenol A by bacteria isolated from desert soils. 3 Biotech 2019; 9: 228.
4. Noszczyńska M, Chodór M, Jałowiecki Ł, Piotrowska-Seget Z. A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12. Biodegradation 2021; 32: 1-15.
5. Lehmler H-J, Liu B, Gadogbe M, Bao W. Exposure to bisphenol A, bisphenol F, and bisphenol S in U.S. adults and children: the national health and nutrition examination survey 2013−2014. ACS Omega 2018; 3: 6523-6532.
6. Eltoukhy A, Jia Y, Nahurira R, Abo-Kadoum MA, Khokhar I, Wang J, et al. Biodegradation of endocrine disruptor Bisphenol A by Pseudomonas putida strain YC-AE1 isolated from polluted soil, Guangdong, China. BMC Microbiol 2020; 20: 11.
7. Rochester JR. Bisphenol A and human health: A review of the literature. Reprod Toxicol 2013; 42: 132-155.
8. Abraham A, Chakraborty P. A review on sources and health impacts of bisphenol A. Rev Environ Health 2020; 35: 201-210.
9. Almeida S, Raposo A, Almeida-González M, Carrascosa C. Bisphenol A: food exposure and impact on human health. Compr Rev Food Sci Food Saf 2018; 17: 1503-1517.
10. Canesi L, Fabbri E. Environmental effects of BPA: focus on aquatic species. Dose Response 2015; 13: 1559325815598304.
11. Repossi A, Farabegoli F, Gazzotti T, Zironi E, Pagliuca G. Bisphenol A in edible part of seafood. Ital J Food Saf 2016; 5: 5666.
12. de Morais Farias J, Krepsky N. Bacterial degradation of bisphenol analogues: an overview. Environ Sci Pollut Res Int 2022; 29: 76543-76564.
13. Mahesh N, Shyamalagowri S, Nithya TG, Aravind J, Govarthanan M, Kamaraj M. Trends and thresholds on bacterial degradation of bisphenol‑A endocrine disruptor - a concise review. Environ Monit Assess 2022; 194: 886.
14. Li G, Zu L, Wong P, Hui X, Lu Y, Xiong J, et al. Biodegradation and detoxification of bisphenol A with one newly-isolated strain Bacillus sp. GZB: Kinetics, mechanism and estrogenic transition. Bioresour Technol 2012; 114: 224-230.
15. Huang C, Xu P, Zeng G, Huang D, Lai C, Cheng M, et al. The rapid degradation of bisphenol A induced by the response of indigenous bacterial communities in sediment. Appl Microbiol Biotechnol 2017; 101: 3919-3928.
16. Badiefar L, Yakhchali B, Rodriguez-Couto S, Veloso A, García-Arenzana JM, Matsumura Y, et al. Biodegradation of bisphenol A by the newly-isolated Enterobacter gergoviae strain BYK-7 enhanced using genetic manipulation. RSC Adv 2015; 5: 29563-29572.
17. Kang J-H, Ri N, Kondo F. Streptomyces sp. strain isolated from river water has high bisphenol A degradability. Lett Appl Microbiol 2004; 39: 178-180.
18. Jia Y, Eltoukhy A, Wang J, Li X, Hlaing TS, Aung MM, et al. Biodegradation of bisphenol A by Sphingobium sp. YC-JY1 and the essential role of cytochrome P450 monooxygenase. Int J Mol Sci 2020; 21: 3588.
19. Thathola P, Agnihotri V, Pandey A, Upadhyay SK. Biodegradation of bisphenol A using psychrotolerant bacterial strain Pseudomonas palleroniana GBPI_508. Arch Microbiol 2022; 204: 272.
20. Fini EH, Ayat S, Pahlavan F (2021). Phenolic compounds in the built environment. In: Badria FA. Ed, Phenolic Compounds-Chemistry, Synthesis, Diversity, Non-Conventional Industrial, Pharmaceutical and Therapeutic Applications, 1st ed. IntechOpen, London, UK, pp 1-23.
21. Yordanova G, Godjevargova T, Nenkova R, Ivanova D. Biodegradation of phenol and phenolic derivatives by a mixture of immobilized cells of Aspergillus Awamori and Trichosporon Cutaneum. Biotechnol Biotechnol Equip 2013; 27: 3681-3688.
22. Ike M, Jin CS, Fujita M. Biodegradation of bisphenol A in the aquatic environment. Water Sci Technol 2000; 42: 31-38.
23. Piazzoli A, Breider F, Aquillon CG, Antonelli M, von Gunten U. Specific and total N-nitrosamines formation potentials of nitrogenous micropollutants during chloramination. Water Res 2018; 135: 311-321.
24. Singh BR, Al-Khedhairy AA, Alarifi SA, Musarrat J. Regulatory elements in the 5'region of 16SrRNA gene of Bacillus sp. strain SJ101. Bioinformation 2009; 3: 375-380.
25. Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021; 38: 3022-3027.
26. Cao B, Nagarajan K, Loh K-C. Biodegradation of aromatic compounds: current status and opportunities for biomolecular approaches. Appl Microbiol Biotechnol 2009; 85: 207-228.
27. Kamaraj M, Sivaraj R, Venckatesh R. Biodegradation of bisphenol A by the tolerant bacterial species isolated from coastal regions of Chennai, Tamil Nadu, India. Int Biodeterior Biodegradation 2014; 93: 216-222.
28. Vijayalakshmi V, Senthilkumar P, Mophin-Kani K, Sivamani S, Sivarajasekar N, Vasantharaj S. Bio-degradation of Bisphenol A by Pseudomonas aeruginosa PAb1 isolated from effluent of thermal paper industry: Kinetic modeling and process optimization. J Radiat Res Appl Sci 2018; 11: 56-65.
29. Heidari H, Sedighi M, Zamir SM, Shojaosadati SA. Bisphenol A degradation by Ralstonia eutropha in the absence and presence of phenol. Int Biodeterior Biodegradation 2017; 119: 37-42.
30. Fouda A. Biodegradation of Bisphenol A by some bacterial species and significance role of plasmids. Int J Adv Res Biol Sci 2015; 2: 93-108.
31. Soghandi B, Salimi F. Study on amendment of rapeseed meal, soybean meal, and NPK fertilizer as biostimulants in bioremediation of diesel-contaminated soil by autochthonous microorganisms. Soil Sediment Contam 2023; 32: 1-21.
2. Suyamud B, Inthorn D, Panyapinyopol B, Thiravetyan P. Biodegradation of Bisphenol A by a newly isolated Bacillus megaterium strain ISO-2 from a polycarbonate industrial wastewater. Water Air Soil Pollut 2018; 229: 348.
3. Louati I, Dammak M, Nasri R, Belbahri L, Nasri M, Abdelkafi S, et al. Biodegradation and detoxifcation of bisphenol A by bacteria isolated from desert soils. 3 Biotech 2019; 9: 228.
4. Noszczyńska M, Chodór M, Jałowiecki Ł, Piotrowska-Seget Z. A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12. Biodegradation 2021; 32: 1-15.
5. Lehmler H-J, Liu B, Gadogbe M, Bao W. Exposure to bisphenol A, bisphenol F, and bisphenol S in U.S. adults and children: the national health and nutrition examination survey 2013−2014. ACS Omega 2018; 3: 6523-6532.
6. Eltoukhy A, Jia Y, Nahurira R, Abo-Kadoum MA, Khokhar I, Wang J, et al. Biodegradation of endocrine disruptor Bisphenol A by Pseudomonas putida strain YC-AE1 isolated from polluted soil, Guangdong, China. BMC Microbiol 2020; 20: 11.
7. Rochester JR. Bisphenol A and human health: A review of the literature. Reprod Toxicol 2013; 42: 132-155.
8. Abraham A, Chakraborty P. A review on sources and health impacts of bisphenol A. Rev Environ Health 2020; 35: 201-210.
9. Almeida S, Raposo A, Almeida-González M, Carrascosa C. Bisphenol A: food exposure and impact on human health. Compr Rev Food Sci Food Saf 2018; 17: 1503-1517.
10. Canesi L, Fabbri E. Environmental effects of BPA: focus on aquatic species. Dose Response 2015; 13: 1559325815598304.
11. Repossi A, Farabegoli F, Gazzotti T, Zironi E, Pagliuca G. Bisphenol A in edible part of seafood. Ital J Food Saf 2016; 5: 5666.
12. de Morais Farias J, Krepsky N. Bacterial degradation of bisphenol analogues: an overview. Environ Sci Pollut Res Int 2022; 29: 76543-76564.
13. Mahesh N, Shyamalagowri S, Nithya TG, Aravind J, Govarthanan M, Kamaraj M. Trends and thresholds on bacterial degradation of bisphenol‑A endocrine disruptor - a concise review. Environ Monit Assess 2022; 194: 886.
14. Li G, Zu L, Wong P, Hui X, Lu Y, Xiong J, et al. Biodegradation and detoxification of bisphenol A with one newly-isolated strain Bacillus sp. GZB: Kinetics, mechanism and estrogenic transition. Bioresour Technol 2012; 114: 224-230.
15. Huang C, Xu P, Zeng G, Huang D, Lai C, Cheng M, et al. The rapid degradation of bisphenol A induced by the response of indigenous bacterial communities in sediment. Appl Microbiol Biotechnol 2017; 101: 3919-3928.
16. Badiefar L, Yakhchali B, Rodriguez-Couto S, Veloso A, García-Arenzana JM, Matsumura Y, et al. Biodegradation of bisphenol A by the newly-isolated Enterobacter gergoviae strain BYK-7 enhanced using genetic manipulation. RSC Adv 2015; 5: 29563-29572.
17. Kang J-H, Ri N, Kondo F. Streptomyces sp. strain isolated from river water has high bisphenol A degradability. Lett Appl Microbiol 2004; 39: 178-180.
18. Jia Y, Eltoukhy A, Wang J, Li X, Hlaing TS, Aung MM, et al. Biodegradation of bisphenol A by Sphingobium sp. YC-JY1 and the essential role of cytochrome P450 monooxygenase. Int J Mol Sci 2020; 21: 3588.
19. Thathola P, Agnihotri V, Pandey A, Upadhyay SK. Biodegradation of bisphenol A using psychrotolerant bacterial strain Pseudomonas palleroniana GBPI_508. Arch Microbiol 2022; 204: 272.
20. Fini EH, Ayat S, Pahlavan F (2021). Phenolic compounds in the built environment. In: Badria FA. Ed, Phenolic Compounds-Chemistry, Synthesis, Diversity, Non-Conventional Industrial, Pharmaceutical and Therapeutic Applications, 1st ed. IntechOpen, London, UK, pp 1-23.
21. Yordanova G, Godjevargova T, Nenkova R, Ivanova D. Biodegradation of phenol and phenolic derivatives by a mixture of immobilized cells of Aspergillus Awamori and Trichosporon Cutaneum. Biotechnol Biotechnol Equip 2013; 27: 3681-3688.
22. Ike M, Jin CS, Fujita M. Biodegradation of bisphenol A in the aquatic environment. Water Sci Technol 2000; 42: 31-38.
23. Piazzoli A, Breider F, Aquillon CG, Antonelli M, von Gunten U. Specific and total N-nitrosamines formation potentials of nitrogenous micropollutants during chloramination. Water Res 2018; 135: 311-321.
24. Singh BR, Al-Khedhairy AA, Alarifi SA, Musarrat J. Regulatory elements in the 5'region of 16SrRNA gene of Bacillus sp. strain SJ101. Bioinformation 2009; 3: 375-380.
25. Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021; 38: 3022-3027.
26. Cao B, Nagarajan K, Loh K-C. Biodegradation of aromatic compounds: current status and opportunities for biomolecular approaches. Appl Microbiol Biotechnol 2009; 85: 207-228.
27. Kamaraj M, Sivaraj R, Venckatesh R. Biodegradation of bisphenol A by the tolerant bacterial species isolated from coastal regions of Chennai, Tamil Nadu, India. Int Biodeterior Biodegradation 2014; 93: 216-222.
28. Vijayalakshmi V, Senthilkumar P, Mophin-Kani K, Sivamani S, Sivarajasekar N, Vasantharaj S. Bio-degradation of Bisphenol A by Pseudomonas aeruginosa PAb1 isolated from effluent of thermal paper industry: Kinetic modeling and process optimization. J Radiat Res Appl Sci 2018; 11: 56-65.
29. Heidari H, Sedighi M, Zamir SM, Shojaosadati SA. Bisphenol A degradation by Ralstonia eutropha in the absence and presence of phenol. Int Biodeterior Biodegradation 2017; 119: 37-42.
30. Fouda A. Biodegradation of Bisphenol A by some bacterial species and significance role of plasmids. Int J Adv Res Biol Sci 2015; 2: 93-108.
31. Soghandi B, Salimi F. Study on amendment of rapeseed meal, soybean meal, and NPK fertilizer as biostimulants in bioremediation of diesel-contaminated soil by autochthonous microorganisms. Soil Sediment Contam 2023; 32: 1-21.
| Files | ||
| Issue | Vol 15 No 6 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i6.14133 | |
| Keywords | ||
| Biodegradation; Bisphenol A; Pseudomonas; Soil | ||
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How to Cite
1.
Chamak N, Farrokh P, Rostami R, Salimi F. Isolation and characterization of a bisphenol A-degrading strain, Pseudomonas aeruginosa DU2, from soil containing decaying plants. Iran J Microbiol. 2023;15(6):734-741.
