Anticancer, antioxidant, and antibacterial effects of nanoemulsion of Origanum majorana essential oil
-
Fatemeh Rasti
,
Elahe Ahmadi
,
Mojdeh Safari
,
Abbas Abdollahi
,
Saha Satvati
,
Razie Ranjbar
,
Mahmoud Osanloo
Abstract
Background and Objectives: This study aimed to develop a natural nanoemulsion with antibacterial and anticancer properties.
Materials and Methods: The chemical composition of the Origanum majorana essential oil was investigated using GC–MS analysis. Besides, the successful loading of the essential oil in the nanoemulsion was confirmed using ATR-FTIR analysis. Moreover, nanoemulsion’s anticancer, antioxidant, and antibacterial activities were investigated.
Results: Terpinen-4-o1 (46.90%) was identified as the major compound in the essential oil. The nanoemulsion with a 149 ± 5 nm droplet size and zeta potential of -11 ± 1 mV was prepared. The cytotoxic effect of the nanoemulsion against A-375 human melanoma cells (IC50 = 139 µg/mL) showed significantly more potency than A-549 human lung cancer cells (IC50 = 318 µg/mL). Interestingly, growth of Staphylococcus aureus (Gram-positive) and E. coli (Gram-negative) bacteria after treatment with 4800 µg/mL of nanoemulsion were obtained at 12 ± 2 and 6 ± 1%, respectively. However, the IC50 value of nanoemulsion against E. coli (580 µg/mL) was not significantly different (P > 0.05) from S. aureus (611 µg/mL).
Conclusion: A straightforward preparation method, high stability, and multi-biological effects are the main advantages of the prepared nanoemulsion. Therefore it could be considered for further investigation in vivo studies or complementary medicine.
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17. Prakash A, Baskaran R, Paramasivam N, Vadivel V. Essential oil based nanoemulsions to improve the microbial quality of minimally processed fruits and vegetables: A review. Food Res Int 2018; 111: 509-523.
18. Kumar M, Bishnoi RS, Shukla AK, Jain CP. Techniques for formulation of nanoemulsion drug delivery system: a review. Prev Nutr Food Sci 2019; 24: 225-234.
19. Moemenbellah-Fard MD, Shahriari-Namadi M, Kelidari HR, Nejad ZB, Ghasemi H, Osanloo M. Chemical composition and repellent activity of nine medicinal essential oils against Anopheles stephensi, the main malaria vector. Int J Trop Insect Sci 2021; 41: 1325-1332.
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21. Plati F, Papi R, Paraskevopoulou A. Characterization of oregano essential oil (Origanum vulgare L. subsp. hirtum) particles produced by the novel nano spray drying technique. Foods 2021; 10: 2923.
22. Ren W, Tian G, Jian S, Gu Z, Zhou L, Yan L, et al. TWEEN coated NaYF 4: Yb, Er/NaYF4 core/shell upconversion nanoparticles for bioimaging and drug delivery. RSC Adv 2012; 2: 7037-7041.
23. Kumari S, Kumaraswamy R, Choudhary RC, Sharma S, Pal A, Raliya R, et al. Thymol nanoemulsion exhibits potential antibacterial activity against bacterial pustule disease and growth promotory effect on soybean. Sci Rep 2018; 8: 6650.
24. Vági E, Simándi B, Suhajda A, Hethelyi E. Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Res Int 2005; 38: 51-57.
25. Shapira S, Pleban S, Kazanov D, Tirosh P, Arber N. Terpinen-4-ol: A novel and promising therapeutic agent for human gastrointestinal cancers. PLoS One 2016; 11(6): e0156540.
26. Malekmohammad K, Sewell RDE, Rafieian-Kopaei M. Antioxidants and atherosclerosis: mechanistic aspects. Biomolecules 2019; 9: 301.
27. Coulombier N, Jauffrais T, Lebouvier N. Antioxidant compounds from microalgae: a review. Mar Drugs 2021; 19: 549.
28. Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract 2012; 2012: 135206.
29. Korrodi-Gregório L, Soto-Cerrato V, Vitorino R, Fardilha M, Pérez-Tomás R. From proteomic analysis to potential therapeutic targets: functional profile of two lung cancer cell lines, A549 and SW900, widely studied in pre-clinical research. PLoS One 2016; 11(11): e0165973.
30. Maksimović-Ivanić D, Bulatović M, Edeler D, Bensing C, Golić I, Korać A, et al. The interaction between SBA-15 derivative loaded with Ph3Sn (CH2) 6OH and human melanoma A375 cell line: uptake and stem phenotype loss. J Biol Inorg Chem 2019; 24: 223-234.
31. Alam A, Ansari MJ, Alqarni MH, Salkini MA, Raish M. Antioxidant, antibacterial, and anticancer activity of ultrasonic nanoemulsion of Cinnamomum Cassia L. essential oil. Plants (Basel) 2023; 12: 834.
32. Panyajai P, Chueahongthong F, Viriyaadhammaa N, Nirachonkul W, Tima S, Chiampanichayakul S, et al. Anticancer activity of Zingiber ottensii essential oil and its nanoformulations. PLoS One 2022; 17(1): e0262335.
33. Navaei Shoorvarzi S, Shahraki F, Shafaei N, Karimi E, Oskoueian E. Citrus aurantium L. bloom essential oil nanoemulsion: Synthesis, characterization, cytotoxicity, and its potential health impacts on mice. J Food Biochem 2020; 44(5): e13181.
34. Mustapa MA, Guswenrivo I, Zuhrotun A, Ikram NKK, Muchtaridi M. Anti-breast cancer activity of essential oil: a systematic review. Appl Sci 2022; 12: 12738.
35. Khoshnevisan K, Alipanah H, Baharifar H, Ranjbar N, Osanloo M. Chitosan nanoparticles containing cinnamomum verum J. Presl essential oil and cinnamaldehyde: preparation, characterization and anticancer effects against melanoma and breast cancer cells. Trad Integr Med 2022; 7: 1-12.
36. Valizadeh A, Khaleghi AA, Roozitalab G, Osanloo M. High anticancer efficacy of solid lipid nanoparticles containing Zataria multiflora essential oil against breast cancer and melanoma cell lines. BMC Pharmacol Toxicol 2021; 22: 52.
37. Seow YX, Yeo CR, Chung HL, Yuk H-G. Plant essential oils as active antimicrobial agents. Crit Rev Food Sci Nutr 2014; 54: 625-644.
38. Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005; 49: 2474-2478.
39. Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules 2020; 25: 1340.
40. Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol 2010; 2: a000414.
2. Thandra KC, Barsouk A, Saginala K, Aluru JS, Barsouk A. Epidemiology of lung cancer. Contemp Oncol (Pozn) 2021; 25: 45-52.
3. Hardy KM, Kirschmann DA, Seftor EA, Margaryan NV, Postovit L-M, Strizzi L, et al. Regulation of the embryonic morphogen Nodal by Notch4 facilitates manifestation of the aggressive melanoma phenotype. Cancer Res 2010; 70: 10340-10350.
4. Schadendorf D, Fisher DE, Garbe C, Gershenwald JE, Grob J-J, Halpern A, et al. Melanoma. Nat Rev Dis Primers 2015; 1: 15003.
5. Housman G, Byler S, Heerboth S, Lapinska K, Longacre M, Snyder N, et al. Drug resistance in cancer: an overview. Cancers (Basel) 2014; 6: 1769-1792.
6. Osanloo M, Ghaznavi G, Abdollahi A. Sureveying the chemical composition and antibacterial activity of essential oils from selected medicinal plants against human pathogens. Iran J Microbiol 2020; 12: 577-583.
7. Cheung GYC, Bae JS, Otto M. Pathogenicity and virulence of Staphylococcus aureus. Virulence 2021; 12: 547-569.
8. Gomes TA, Elias WP, Scaletsky IC, Guth BE, Rodrigues JF, Piazza RM, et al. Diarrheagenic Escherichia coli. Braz J Microbiol 2016; 47 Suppl 1(Suppl 1): 3-30.
9. Noorpisheh Ghadimi S, Sharifi N, Osanloo M. The leishmanicidal activity of essential oils: A systematic review. J Herbmed Pharmacol 2020; 9: 300-308.
10. Osanloo M, Yousefpoor Y, Alipanah H, Ghanbariasad A, Jalilvand M, Amani A. In-vitro assessment of Essential Oils as anticancer therapeutic agents: a systematic literature review. Jordan J Pharm Sci 2022; 15: 173-203.
11. Mossa A-T, Refaie AA, Ramadan A, Bouajila J. Amelioration of prallethrin-induced oxidative stress and hepatotoxicity in rat by the administration of Origanum majorana essential oil. Biomed Res Int 2013; 2013: 859085.
12. Prakash B, Singh P, Kedia A, Dubey NK. Assessment of some essential oils as food preservatives based on antifungal, antiaflatoxin, antioxidant activities and in vivo efficacy in food system. Food Res Int 2012; 49: 201-208.
13. Rao J, Chen B, McClements DJ. Improving the efficacy of essential oils as antimicrobials in foods: Mechanisms of action. Annu Rev Food Sci Technol 2019; 10: 365-387.
14. Barradas TN, de Holanda e Silva KG. Nanoemulsions of essential oils to improve solubility, stability and permeability: a review. Environ Chem Lett 2021; 19: 1153-1171.
15. Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres MdP, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology 2018; 16: 71.
16. Maleki H, Azadi H, Yousefpoor Y, Doostan M, Doostan M, Farzaei MH. Encapsulation of Ginger extract in nanoemulsions: preparation, characterization and in vivo evaluation in rheumatoid arthritis. J Pharm Sci 2023; 112: 1687-1697.
17. Prakash A, Baskaran R, Paramasivam N, Vadivel V. Essential oil based nanoemulsions to improve the microbial quality of minimally processed fruits and vegetables: A review. Food Res Int 2018; 111: 509-523.
18. Kumar M, Bishnoi RS, Shukla AK, Jain CP. Techniques for formulation of nanoemulsion drug delivery system: a review. Prev Nutr Food Sci 2019; 24: 225-234.
19. Moemenbellah-Fard MD, Shahriari-Namadi M, Kelidari HR, Nejad ZB, Ghasemi H, Osanloo M. Chemical composition and repellent activity of nine medicinal essential oils against Anopheles stephensi, the main malaria vector. Int J Trop Insect Sci 2021; 41: 1325-1332.
20. Ranjbar R, Zarenezhad E, Abdollahi A, Nasrizadeh M, Firooziyan S, Namdar N, et al. Nanoemulsion and nanogel containing Cuminum cyminum L Essential Oil: antioxidant, anticancer, antibacterial, and antilarval properties. J Trop Med 2023; 2023: 5075581.
21. Plati F, Papi R, Paraskevopoulou A. Characterization of oregano essential oil (Origanum vulgare L. subsp. hirtum) particles produced by the novel nano spray drying technique. Foods 2021; 10: 2923.
22. Ren W, Tian G, Jian S, Gu Z, Zhou L, Yan L, et al. TWEEN coated NaYF 4: Yb, Er/NaYF4 core/shell upconversion nanoparticles for bioimaging and drug delivery. RSC Adv 2012; 2: 7037-7041.
23. Kumari S, Kumaraswamy R, Choudhary RC, Sharma S, Pal A, Raliya R, et al. Thymol nanoemulsion exhibits potential antibacterial activity against bacterial pustule disease and growth promotory effect on soybean. Sci Rep 2018; 8: 6650.
24. Vági E, Simándi B, Suhajda A, Hethelyi E. Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Res Int 2005; 38: 51-57.
25. Shapira S, Pleban S, Kazanov D, Tirosh P, Arber N. Terpinen-4-ol: A novel and promising therapeutic agent for human gastrointestinal cancers. PLoS One 2016; 11(6): e0156540.
26. Malekmohammad K, Sewell RDE, Rafieian-Kopaei M. Antioxidants and atherosclerosis: mechanistic aspects. Biomolecules 2019; 9: 301.
27. Coulombier N, Jauffrais T, Lebouvier N. Antioxidant compounds from microalgae: a review. Mar Drugs 2021; 19: 549.
28. Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract 2012; 2012: 135206.
29. Korrodi-Gregório L, Soto-Cerrato V, Vitorino R, Fardilha M, Pérez-Tomás R. From proteomic analysis to potential therapeutic targets: functional profile of two lung cancer cell lines, A549 and SW900, widely studied in pre-clinical research. PLoS One 2016; 11(11): e0165973.
30. Maksimović-Ivanić D, Bulatović M, Edeler D, Bensing C, Golić I, Korać A, et al. The interaction between SBA-15 derivative loaded with Ph3Sn (CH2) 6OH and human melanoma A375 cell line: uptake and stem phenotype loss. J Biol Inorg Chem 2019; 24: 223-234.
31. Alam A, Ansari MJ, Alqarni MH, Salkini MA, Raish M. Antioxidant, antibacterial, and anticancer activity of ultrasonic nanoemulsion of Cinnamomum Cassia L. essential oil. Plants (Basel) 2023; 12: 834.
32. Panyajai P, Chueahongthong F, Viriyaadhammaa N, Nirachonkul W, Tima S, Chiampanichayakul S, et al. Anticancer activity of Zingiber ottensii essential oil and its nanoformulations. PLoS One 2022; 17(1): e0262335.
33. Navaei Shoorvarzi S, Shahraki F, Shafaei N, Karimi E, Oskoueian E. Citrus aurantium L. bloom essential oil nanoemulsion: Synthesis, characterization, cytotoxicity, and its potential health impacts on mice. J Food Biochem 2020; 44(5): e13181.
34. Mustapa MA, Guswenrivo I, Zuhrotun A, Ikram NKK, Muchtaridi M. Anti-breast cancer activity of essential oil: a systematic review. Appl Sci 2022; 12: 12738.
35. Khoshnevisan K, Alipanah H, Baharifar H, Ranjbar N, Osanloo M. Chitosan nanoparticles containing cinnamomum verum J. Presl essential oil and cinnamaldehyde: preparation, characterization and anticancer effects against melanoma and breast cancer cells. Trad Integr Med 2022; 7: 1-12.
36. Valizadeh A, Khaleghi AA, Roozitalab G, Osanloo M. High anticancer efficacy of solid lipid nanoparticles containing Zataria multiflora essential oil against breast cancer and melanoma cell lines. BMC Pharmacol Toxicol 2021; 22: 52.
37. Seow YX, Yeo CR, Chung HL, Yuk H-G. Plant essential oils as active antimicrobial agents. Crit Rev Food Sci Nutr 2014; 54: 625-644.
38. Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005; 49: 2474-2478.
39. Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules 2020; 25: 1340.
40. Silhavy TJ, Kahne D, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol 2010; 2: a000414.
| Files | ||
| Issue | Vol 15 No 4 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i4.13511 | |
| Keywords | ||
| Nanotechnology; Skin neoplasms; Lung neoplasms; Anti-bacterial agents | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Rasti F, Ahmadi E, Safari M, Abdollahi A, Satvati S, Ranjbar R, Osanloo M. Anticancer, antioxidant, and antibacterial effects of nanoemulsion of Origanum majorana essential oil. Iran J Microbiol. 2023;15(4):565-573.
