Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut
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Abstract
Background and Objectives: The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of interest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry’s gut.
Materials and Methods: Probiotic characteristics such as hemolysis activity, acid, bile, gastric juice tolerate, in vitro adhesion assay, cell surface properties (hydrophobicity, auto-aggregation and co-aggregation) and antibiotic susceptibility test were evaluated. Finally, selected isolates identified molecularly after temperature-salt tolerance and extracellular enzyme activity (amylase, protease and cellulose).
Results: Out of 362 strains isolated from native poultry in three geographical regions of Iran, nine strains (belong to Bacillus sp., Enterococcus sp., Pediococcus sp., Lactobacillus sp., Kluyveromyces sp.) showed resistance against gastrointestinal physiological conditions, desirable surface properties, ability to adhere to epithelial intestine cell line and antibiotic susceptibility. Also, these strains were discovered to be temperature-salt tolerant but, only a small number of them were able to produce hydrolase enzymes.
Conclusion: According to the results, the selected strains can be introduced as native probiotic candidates for utilization in novel poultry feeds.
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19. Celiberto LS, Pinto RA, Rossi EA, Vallance BA, Cavallini DCU. Isolation and characterization of potentially probiotic bacterial strains from mice: Proof of concept for personalized probiotics. Nutrients 2018; 10: 1684.
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24. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y, Seo H, et al. Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613-1617.
25. Casarotti SN, Carneiro BM, Todorov SD, Nero LA, Rahal P, Penna AL. In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese. Ann Microbiol 2017; 67:289-301.
26. Rajoka MSR, Mehwish HM, Hayat HF, Hussain N, Sarwar S, Aslam H, et al. Characterization, the antioxidant and antimicrobial activity of exopolysaccharide isolated from poultry origin lactobacilli. Probiotics
Antimicrob Proteins 2019; 11: 1132-1142.
27. Damayanti E, Istiqomah L, Saragih JE, Purwoko T, Sardjono. Characterization of lactic acid bacteria as poultry probiotic candidates with aflatoxin B1 binding activities. IOP Conf Ser Earth Environ Sci 2017; 101: 012030.
28. Pisano MB, Viale S, Conti S, Fadda ME, Deplano M, Melis MP, et al. Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products. Biomed Res Int 2014; 2014: 286390.
29. Caggia C, De Angelis M, Pitino I, Pino A, Randazzo CL. Probiotic features of Lactobacillus strains isolated from Ragusano and Pecorino Siciliano cheeses. Food Microbiol 2015; 50: 109-117.
30. Karimi Torshizi MA, Moghaddam AR, Rahimi SH, Mojgani N. Assessing the effect of administering probiotics in water or as a feed supplement on broiler performance and immune response. Br Poult Sci 2010; 51: 178-184.
31. Sebastian AP, Keerthi TR. Adhesion and cell surface properties of wild species of spore formers against enteric pathogens. Asian Pac J Trop Med 2013; 6: 110-114.
32. Shehata MG, El Sohaimy SA, El-sahn MA, Youssef MM. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Ann Agric Sci 2016; 61: 65-75.
33. Kobierecka PA, Wyszyńska AK, Aleksandrzak-Piekarczyk T, Kuczkowski M, Tuzimek A, Piotrowska W, et al. In vitro characteristics of Lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of Campylobacter colonization. Microbiologyopen 2017; 6(5): e00512.
2. Reuben RC, Roy PC, Sarkar SL, Alam R, Jahid IK. Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics. BMC Microbiol 2019; 19: 253.
3. Shokryazdan P, Faseleh Jahromi M, Liang JB, Ho YW. Probiotics: From isolation to application. J Am Coll Nutr 2017; 36: 666-676.
4. Rahmani B, Alimadadi N, Attaran B, Nasr S. Yeasts from Iranian traditional milk kefir samples : isolation, molecular identification and their potential probiotic properties. Lett Appl Microbiol 2022; 75: 1264-1274.
5. Shehata AA, Yalçın S, Latorre JD, Basiouni S, Attia YA, El-wahab AA, et al. Probiotics, prebiotics, and phytogenic substances for optimizing gut health in poultry. Microorganisms 2022; 10: 395.
6. Adhikari PA, Kim WK. Overview of prebiotics and probiotics: focus on performance, gut health and immunity – a review. Ann Anim Sci 2017; 17: 949-966.
7. Van Zyl WF, Deane SM, Dicks LMT. Molecular insights into probiotic mechanisms of action employed against intestinal pathogenic bacteria. Gut Microbes 2020; 12: 1831339.
8. Guli M, Winarsih S, Barlianto W, Illiandri O, Sumarno SP. Mechanism of Lactobacillus reuteri probiotic in increasing intestinal mucosal immune system. Open Access Maced J Med Sci 2021; 9: 784-793.
9. Penaloza-Vazquez A, Ma LM, Rayas-Duarte P. Isolation and characterization of Bacillus spp. strains as potential probiotics for poultry. Can J Microbiol 2019; 65: 762-774.
10. Ramlucken U, Roets Y, Ramchuran SO, Moonsamy G, van Rensburg CJ, Thantsha MS, et al. Isolation, selection and evaluation of Bacillus spp. As potential multi-mode probiotics for poultry. J Gen Appl Microbiol 2020; 66: 228-238.
11. Rajoka MSR, Hayat HF, Sarwar S, Mehwish HM, Ahmad F, Hussain N, et al. Isolation and evaluation of probiotic potential of Lactic Acid Bacteria isolated from poultry intestine. Microbiolgy 2018; 87: 116-126.
12. Hidayati Martin RS, Laconi EB, Jayanegara A, Sofyan A, Istiqomah L. Activity and viability of probiotic candidates consisting of lactic acid bacteria and yeast isolated from native poultry gastrointestinal tract. AIP Conf Proc 2018; 2021: 070012.
13. Saikot FK, Khaledur Rahman SM, Forhad MH. Probiotic properties analysis of isolated lactic acid bacteria from buffalo milk. Arch Clin Microbiol 2015; 7: 5-10.
14. Utama CS, Hanim C, Zuprizal Z.Probiotic testing of Lactobacillus brevis and Lactobacillus plantarum from fermented cabbage waste juice. Pakistan J Nutr 2018; 17: 323-328.
15. Reuben RC, Roy PC, Sarkar SL, Alam RU, Jahid IK. Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics. BMC Microbiol 2019; 19: 253.
16. Manhar AK, Saikia D, Bashir Y, Mech RK, Nath D, Konwar BK, et al. In vitro evaluation of celluloytic Bacillus amyloliquefaciens AMS1 isolated from traditional fermented soybean (Churpi) as an animal probiotic. Res Vet Sci 2015; 99: 149-156.
17. Kavitha M, Raja M, Perumal P. Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu (Hamilton, 1822). Aquac Reports 2018; 11: 59-69.
18. Honey Chandran C, Keerthi TR. Probiotic potency of Lactobacillus plantarum KX519413 and KX519414 isolated from honey bee gut. FEMS Microbiol Lett 2018; 365: 10.1093/femsle/fnx285.
19. Celiberto LS, Pinto RA, Rossi EA, Vallance BA, Cavallini DCU. Isolation and characterization of potentially probiotic bacterial strains from mice: Proof of concept for personalized probiotics. Nutrients 2018; 10: 1684.
20. Gupta M, Bajaj BK. Functional characterization of potential probiotic lactic acid bacteria isolated from Kalarei and development of probiotic fermented oat flour. Probiotics Antimicrob Proteins 2018; 10: 654-661.
21. Pundir RK, Rana S, Kashyap N, Kaur A. Probiotic potential of lactic acid bacteria isolated from food samples: An in vitro study. J Appl Pharm Sci 2013; 3: 85-93.
22. Powthong P, Suntornthiticharoen P. Antimicrobial and enzyme activity produced by Bacillus Spp. isolated from soil. Int J Pharm Pharm Sci 2017; 9: 205.
23. Beganovi J, Kos B, Lebos Pavunc A, Uroic K, Dzidara P, Suskovic J. Proteolytic activity of probiotic strain Lactobacillus helveticus M92. Anaerobe 2013; 20: 58-64.
24. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y, Seo H, et al. Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613-1617.
25. Casarotti SN, Carneiro BM, Todorov SD, Nero LA, Rahal P, Penna AL. In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese. Ann Microbiol 2017; 67:289-301.
26. Rajoka MSR, Mehwish HM, Hayat HF, Hussain N, Sarwar S, Aslam H, et al. Characterization, the antioxidant and antimicrobial activity of exopolysaccharide isolated from poultry origin lactobacilli. Probiotics
Antimicrob Proteins 2019; 11: 1132-1142.
27. Damayanti E, Istiqomah L, Saragih JE, Purwoko T, Sardjono. Characterization of lactic acid bacteria as poultry probiotic candidates with aflatoxin B1 binding activities. IOP Conf Ser Earth Environ Sci 2017; 101: 012030.
28. Pisano MB, Viale S, Conti S, Fadda ME, Deplano M, Melis MP, et al. Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products. Biomed Res Int 2014; 2014: 286390.
29. Caggia C, De Angelis M, Pitino I, Pino A, Randazzo CL. Probiotic features of Lactobacillus strains isolated from Ragusano and Pecorino Siciliano cheeses. Food Microbiol 2015; 50: 109-117.
30. Karimi Torshizi MA, Moghaddam AR, Rahimi SH, Mojgani N. Assessing the effect of administering probiotics in water or as a feed supplement on broiler performance and immune response. Br Poult Sci 2010; 51: 178-184.
31. Sebastian AP, Keerthi TR. Adhesion and cell surface properties of wild species of spore formers against enteric pathogens. Asian Pac J Trop Med 2013; 6: 110-114.
32. Shehata MG, El Sohaimy SA, El-sahn MA, Youssef MM. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Ann Agric Sci 2016; 61: 65-75.
33. Kobierecka PA, Wyszyńska AK, Aleksandrzak-Piekarczyk T, Kuczkowski M, Tuzimek A, Piotrowska W, et al. In vitro characteristics of Lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of Campylobacter colonization. Microbiologyopen 2017; 6(5): e00512.
| Files | ||
| Issue | Vol 15 No 2 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i2.12479 | |
| Keywords | ||
| Antibiotic; Bacillus sp; In vitro test; Poultry; Probiotic | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Makzum S, Ghadam P, Ramezani M. Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut. Iran J Microbiol. 2023;15(2):267-277.
