Plant growth promoting and antagonistic traits of bacteria isolated from forest soil samples
,
Abstract
Background and Objectives: Sustainability in agricultural systems without compromising the environmental quality and conservation is one of the major concerns of today’s world. The excessive use of agrochemicals is posing serious threats to the environment. Therefore identification of efficient plant growth promoting (PGP) bacteria as an alternative to chemically synthesized fertilizers is of great interest.
Materials and Methods: In the present investigation, forest soil samples collected were used for isolation of efficient plant growth promoting bacteria.
Results: Total of 14 bacteria were isolated, and tested for various PGP properties. Out of the 14 isolates, four isolates labelled as BKOU-1, BKOU-8, BKOU-13 and BKOU-14 showed significant plant growth promoting traits, hydrolytic enzyme production and effectively restricted the mycelial development of phyto-pathogenic fungi (Fusarium oxysporum and Macrophomina phaseolina). 16 S rRNA gene sequences of the bacterial isolates BKOU-1, BKOU-8, BKOU-13 and BKOU-14 were found to have maximum identity with Bacillus aerius, Bacillus infantis, Alcaligenes faecalis and Klebsiella Oxytoca respectively. All four bacterial isolates nucleotide sequences were submitted to GenBank and NCBI accession numbers were generated as follows: OL721916, OL721918, OL721919 and OL721926.
Conclusion: According to the findings of the study, these PGPR could be employed as biofertilizers/ biopesticides to boost crop yield of different crops in sustainable manner.
1. Sharma K, Sharma S, Prasad SR. PGPR: renewable tool for sustainable agriculture. Int J Curr Microbiol App Sci 2019; 8: 525-530.
2. Mir MI, Hameeda B, Quadriya H, Kumar BK, Ilyas N, Kee Zuan AT, et al. Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) rhizosphere and their effect on plant growth promotion. Front Nutr 2022; 8: 781764.
3. Alori ET, Babalola OO. Microbial inoculants for improving crop quality and human health in Africa. Front Microbiol 2018; 9: 2213.
4. Ahmad I, Zaib S (2020). Mighty microbes: plant growth promoting microbes in soil health and sustainable agriculture. In Soil Health Springer, Cham. pp 243-264.
5. Dutta S, Podile AR. Plant growth promoting rhizobacteria (PGPR): the bugs to debug the root zone. Crit Rev Microbiol 2010; 36: 232-244.
6. Bandyopadhyay P, Bhuyan SK, Yadava PK, Varma A, Tuteja N. Emergence of plant and rhizospheric microbiota as stable interactomes. Protoplasma 2017; 254: 617-626.
7. Ahkami AH, White III RA, Handakumbura PP, Jansson C. Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity. Rhizosphere 2017; 3: 233-243.
8. Souza RD, Ambrosini A, Passaglia LM. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet Mol Biol 2015; 38: 401-419.
9. Pascale A, Proietti S, Pantelides IS, Stringlis IA. Modulation of the root microbiome by plant molecules: the basis for targeted disease suppression and plant growth promotion. Front Plant Sci 2020; 10: 1741.
10. Singh VK, Singh AK, Kumar A. Disease management of tomato through PGPB: current trends and future perspective. 3 Biotech 2017; 7: 255.
11. Glick BR (2020). Introduction to plant growth-promoting bacteria. In Beneficial plant-bacterial interactions. Springer, Cham. pp: 1-37.
12. Basu A, Prasad P, Das SN, Kalam S, Sayyed RZ, Reddy MS, et al. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability 2021; 13: 1140.
13. Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, et al. Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Front Plant Sci 2018; 9: 1473.
14. Suleman M, Yasmin S, Rasul M, Yahya M, Atta BM, Mirza MS. Phosphate solubilizing bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat. PLoS One 2018; 13(9): e0204408.
15. Mishra J, Arora NK. Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture. Appl Soil Ecol 2018; 125: 35-45.
16. Singh S, Kumar V, Sidhu GK, Datta S, Dhanjal DS, Koul B, et al. Plant growth promoting rhizobacteria from heavy metal contaminated soil promote growth attributes of Pisum sativum L. Biocatal Agric Biotechnol 2019; 17: 665-671.
17. Ahemad M, Kibret M. Mechanisms and applications of plant growth promoting rhizobacteria: current perspective. J King Saud Univ Sci 2014; 26: 1-20.
18. Kumar A, Singh VK, Tripathi V, Singh PP, Singh AK. Plant growth-promoting rhizobacteria (PGPR): perspective in agriculture under biotic and abiotic stress. In Crop Improvement Through Microbial Biotechnol 2018; pp. 333-342.
19. Nevita T, Sharma GD, Pandey P. Composting of rice-residues using lignocellulolytic plant-probiotic Stenotrophomonas maltophilia, and its evaluation for growth enhancement of Oryza sativa L. Environ Sustain 2018; 1: 185-196.
20. Rasool A, Mir MI, Zulfajri M, Hanafiah MM, Unnisa SA, Mahboob M. Plant growth promoting and antifungal asset of indigenous rhizobacteria secluded from saffron (Crocus sativus L.) rhizosphere. Microb Pathog 2021; 150: 104734.
21. Hamid B, Zaman M, Farooq S, Fatima S, Sayyed RZ, Baba ZA, et al. Bacterial plant biostimulants: A sustainable way towards improving growth, productivity, and health of crops. Sustainability 2021; 13: 2856.
22. Louden BC, Haarmann D, Lynne AM. Use of blue agar CAS assay for siderophore detection. J Microbiol Biol Educ 2011; 12: 51-53.
23. Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 2008; 163: 173-181.
24. Di Benedetto NA, Campaniello D, Bevilacqua A, Cataldi MP, Sinigaglia M, Flagella Z, et al. Isolation, screening, and characterization of plant-growth-promoting bacteria from durum wheat rhizosphere to improve N and P nutrient use efficiency. Microorganisms 2019; 7: 541.
25. Mehta S, Nautiyal CS. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr Microbiol 2001; 43: 51-56.
26. Penrose DM, Glick BR. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant 2003; 118: 10-15.
27. Bhattacharya A, Chandra S, Barik S. Lipase and protease producing microbes from the environment of sugar beet field. Indian J Agric Biochem 2009; 22: 26-30.
28. Yassin SN, Jiru TM, Indracanti M. Screening and characterization of thermostable amylase-producing bacteria isolated from soil samples of afdera, Afar region, and molecular detection of amylase-coding gene. Int J Microbiol 2021; 2021: 5592885.
29. Pandey P, Kang SC, Maheswari DK. Isolation of endophytic plant growth promoting Burkholderia spp. MSSP from root nodules of Mimosa pudica. Curr Sci 2005; 89: 177-180.
30. Zakhia F, Jeder H, Domergue O, Willems A, Cleyet-Marel JC, Gillis M, et al. Characterisation of wild legume nodulating bacteria (LNB) in the infra-arid zone of Tunisia. Syst Appl Microbiol 2004; 27: 380-395.
31. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35: 1547-1549.
32. Smalla K, Sessitsch A, Hartmann A. The Rhizosphere: soil compartment influenced by the root. FEMS Microbiol Ecol 2006; 56: 165.
33. Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): Emergence in agriculture. World J Microbiol Biotechnol 2012; 28: 1327-1350.
34. Shameer S, Prasad TNVKV. Plant growth promoting rhizobacteria for sustainable agricultural practices with special reference to biotic and abiotic stresses. Plant Growth Regul 2018; 84: 603-615.
35. Tsegaye Z, Gizaw B, Tefera G, Feleke A, Chaniyalew S, Alemu T, et al. Isolation and biochemical characterization of plant growth promoting (PGP) bacteria colonizing the rhizosphere of Tef crop during the seedling stage. J plant Sci phytopathol 2019; 3: 13-27.
36. Patten CL, Glick BR. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Appl Environ Microbiol 2002; 68: 3795-3801.
37. Bhardwaj G, Shah R, Joshi B, Patel P. Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004. J App Biol Biotech 2017; 5: 47-52.
38. Benaissa A, Djebbar R, Abderrahmani A. Diversity of plant growth promoting Rhizobacteria of Rhus tripartitus in arid soil of Algeria (Ahaggar) and their physiological properties under abiotic stresses. Adv Hortic Sci 2018; 32: 525-534.
39. Kakar KU, Nawaz Z, Cui Z, Almoneafy AA, Ullah R, Shu Q-Y. Rhizosphere‐associated Alcaligenes and Bacillus strains that induce resistance against blast and sheath blight diseases enhance plant growth and improve mineral content in rice. J Appl Microbiol 2018; 124: 779-796.
40. Miljaković D, Marinković J, Balešević-Tubić S. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms 2020; 8: 1037.
41. Manasa M, Ravinder P, Gopalakrishnan S, Srinivas V, Sayyed RZ, El Enshasy HA, et al. Co-inoculation of Bacillus spp. for growth promotion and iron fortification in sorghum. Sustainability 2021; 13: 12091.
42. Hyder S, Gondal AS, Rizvi ZF, Ahmad R, Alam MM, Hannan A, et al. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Sci Rep 2020; 10: 13859.
43. Liu M, Liu X, Cheng B-S, Ma X-L, Lyu X-T, Zhao X-F, et al. Fang. Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers. Span J Agric Res 2016; 14(4): e1106.
44. Dasgupta D, Sengupta C, Paul G. Screening and identification of best three phosphate solubilizing and IAA producing PGPR inhabiting the rhizosphere of Sesbania bispinosa. Int J Innov Res Sci Eng Technol 2015; 4: 3968-3979.
45. Wan W, Qin Y, Wu H, Zuo W, He H, Tan J, et al. Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil. Front Microbiol 2020; 11: 752.
46. Babar M, Saif-Ur-Rehman, Rasul S, Aslam K, Abbas R, Athar HU, et al. Mining of halo-tolerant plant growth promoting rhizobacteria and their impact on wheat (Triticum aestivum L.) under saline conditions. J King Saud Univ Sci 2021; 33: 101372.
47. Baig KS, Arshad M, Shaharoona B, Khalid A, Ahmed I. Comparative effectiveness of Bacillus spp. possessing either dual or single growth-promoting traits for improving phosphorus uptake, growth and yield of wheat (Triticum aestivum L.). Ann Microbiol 2012; 62: 1109-1119.
48. Lee EY, Hong SH. Plant growth-promoting ability by the newly isolated bacterium Bacillus aerius MH1RS1 from indigenous plant in sand dune. J Korean Soc Environ Eng 2013; 35: 687-693.
49. Abo-Zaid GA, Soliman NAM, Abdullah AS, El-Sharouny EE, Matar SM, Sabry SAF. Maximization of siderophores production from biocontrol agents, Pseudomonas aeruginosa f2 and Pseudomonas fluorescens JY3 using batch and exponential fed-batch fermentation. Processes 2020; 8: 455.
50. Choudhary DK, Johri BN. Interactions of Bacillus spp. and plants - with special reference to induced systemic resistance (ISR). Microbiol Res 2009; 164: 493-513.
51. Marques APGC, Pires C, Moreira H, Rangel AOSS, Castro PML. Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biol Biochem 2010; 42: 1229-1235.
52. Swamy MK, Akhtar MS, Sinniah UR. Response of PGPR and AM fungi toward growth and secondary metabolite production in medicinal and aromatic plants. In Plant Soil Microbes, Springer: Cham, Switzerland 2016; pp. 145-168.
53. Guleria S, Walia A, Chauhan A, Shirkot CK. Molecular characterization of alkaline protease of Bacillus amyloliquefaciens SP1 involved in biocontrol of Fusarium oxysporum. Int J Food Microbiol 2016; 232: 134-143.
54. Khan MS, Gao J, Zhang M, Chen X, Moe TS, Du Y, et al. Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii. 3 Biotech 2020; 10: 305.
55. Castaldi S, Masi M, Sautua F, Cimmino A, Isticato R, Carmona M, et al. Pseudomonas fluorescens showing antifungal activity against Macrophomina phaseolina, a severe pathogenic fungus of soybean, produces phenazine as the main active metabolite. Biomolecules 2021; 11: 1728.
56. Kumari P, Bishnoi SK, Chandra S. Assessment of antibiosis potential of Bacillus sp. against the soil-borne fungal pathogen Sclerotium rolfsii Sacc. (Athelia rolfsii (Curzi) Tu & Kimbrough). Egypt J Biol Pest Control 2021; 31: 1-11.
57. Sharf W, Javaid A, Shoaib A, Khan IH. Induction of resistance in chili against Sclerotium rolfsii by plant-growth-promoting rhizobacteria and Anagallis arvensis. Egypt J Biol Pest Control 2021; 31: 1-11.
2. Mir MI, Hameeda B, Quadriya H, Kumar BK, Ilyas N, Kee Zuan AT, et al. Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) rhizosphere and their effect on plant growth promotion. Front Nutr 2022; 8: 781764.
3. Alori ET, Babalola OO. Microbial inoculants for improving crop quality and human health in Africa. Front Microbiol 2018; 9: 2213.
4. Ahmad I, Zaib S (2020). Mighty microbes: plant growth promoting microbes in soil health and sustainable agriculture. In Soil Health Springer, Cham. pp 243-264.
5. Dutta S, Podile AR. Plant growth promoting rhizobacteria (PGPR): the bugs to debug the root zone. Crit Rev Microbiol 2010; 36: 232-244.
6. Bandyopadhyay P, Bhuyan SK, Yadava PK, Varma A, Tuteja N. Emergence of plant and rhizospheric microbiota as stable interactomes. Protoplasma 2017; 254: 617-626.
7. Ahkami AH, White III RA, Handakumbura PP, Jansson C. Rhizosphere engineering: Enhancing sustainable plant ecosystem productivity. Rhizosphere 2017; 3: 233-243.
8. Souza RD, Ambrosini A, Passaglia LM. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet Mol Biol 2015; 38: 401-419.
9. Pascale A, Proietti S, Pantelides IS, Stringlis IA. Modulation of the root microbiome by plant molecules: the basis for targeted disease suppression and plant growth promotion. Front Plant Sci 2020; 10: 1741.
10. Singh VK, Singh AK, Kumar A. Disease management of tomato through PGPB: current trends and future perspective. 3 Biotech 2017; 7: 255.
11. Glick BR (2020). Introduction to plant growth-promoting bacteria. In Beneficial plant-bacterial interactions. Springer, Cham. pp: 1-37.
12. Basu A, Prasad P, Das SN, Kalam S, Sayyed RZ, Reddy MS, et al. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability 2021; 13: 1140.
13. Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, et al. Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Front Plant Sci 2018; 9: 1473.
14. Suleman M, Yasmin S, Rasul M, Yahya M, Atta BM, Mirza MS. Phosphate solubilizing bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat. PLoS One 2018; 13(9): e0204408.
15. Mishra J, Arora NK. Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture. Appl Soil Ecol 2018; 125: 35-45.
16. Singh S, Kumar V, Sidhu GK, Datta S, Dhanjal DS, Koul B, et al. Plant growth promoting rhizobacteria from heavy metal contaminated soil promote growth attributes of Pisum sativum L. Biocatal Agric Biotechnol 2019; 17: 665-671.
17. Ahemad M, Kibret M. Mechanisms and applications of plant growth promoting rhizobacteria: current perspective. J King Saud Univ Sci 2014; 26: 1-20.
18. Kumar A, Singh VK, Tripathi V, Singh PP, Singh AK. Plant growth-promoting rhizobacteria (PGPR): perspective in agriculture under biotic and abiotic stress. In Crop Improvement Through Microbial Biotechnol 2018; pp. 333-342.
19. Nevita T, Sharma GD, Pandey P. Composting of rice-residues using lignocellulolytic plant-probiotic Stenotrophomonas maltophilia, and its evaluation for growth enhancement of Oryza sativa L. Environ Sustain 2018; 1: 185-196.
20. Rasool A, Mir MI, Zulfajri M, Hanafiah MM, Unnisa SA, Mahboob M. Plant growth promoting and antifungal asset of indigenous rhizobacteria secluded from saffron (Crocus sativus L.) rhizosphere. Microb Pathog 2021; 150: 104734.
21. Hamid B, Zaman M, Farooq S, Fatima S, Sayyed RZ, Baba ZA, et al. Bacterial plant biostimulants: A sustainable way towards improving growth, productivity, and health of crops. Sustainability 2021; 13: 2856.
22. Louden BC, Haarmann D, Lynne AM. Use of blue agar CAS assay for siderophore detection. J Microbiol Biol Educ 2011; 12: 51-53.
23. Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 2008; 163: 173-181.
24. Di Benedetto NA, Campaniello D, Bevilacqua A, Cataldi MP, Sinigaglia M, Flagella Z, et al. Isolation, screening, and characterization of plant-growth-promoting bacteria from durum wheat rhizosphere to improve N and P nutrient use efficiency. Microorganisms 2019; 7: 541.
25. Mehta S, Nautiyal CS. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr Microbiol 2001; 43: 51-56.
26. Penrose DM, Glick BR. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant 2003; 118: 10-15.
27. Bhattacharya A, Chandra S, Barik S. Lipase and protease producing microbes from the environment of sugar beet field. Indian J Agric Biochem 2009; 22: 26-30.
28. Yassin SN, Jiru TM, Indracanti M. Screening and characterization of thermostable amylase-producing bacteria isolated from soil samples of afdera, Afar region, and molecular detection of amylase-coding gene. Int J Microbiol 2021; 2021: 5592885.
29. Pandey P, Kang SC, Maheswari DK. Isolation of endophytic plant growth promoting Burkholderia spp. MSSP from root nodules of Mimosa pudica. Curr Sci 2005; 89: 177-180.
30. Zakhia F, Jeder H, Domergue O, Willems A, Cleyet-Marel JC, Gillis M, et al. Characterisation of wild legume nodulating bacteria (LNB) in the infra-arid zone of Tunisia. Syst Appl Microbiol 2004; 27: 380-395.
31. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35: 1547-1549.
32. Smalla K, Sessitsch A, Hartmann A. The Rhizosphere: soil compartment influenced by the root. FEMS Microbiol Ecol 2006; 56: 165.
33. Bhattacharyya PN, Jha DK. Plant growth-promoting rhizobacteria (PGPR): Emergence in agriculture. World J Microbiol Biotechnol 2012; 28: 1327-1350.
34. Shameer S, Prasad TNVKV. Plant growth promoting rhizobacteria for sustainable agricultural practices with special reference to biotic and abiotic stresses. Plant Growth Regul 2018; 84: 603-615.
35. Tsegaye Z, Gizaw B, Tefera G, Feleke A, Chaniyalew S, Alemu T, et al. Isolation and biochemical characterization of plant growth promoting (PGP) bacteria colonizing the rhizosphere of Tef crop during the seedling stage. J plant Sci phytopathol 2019; 3: 13-27.
36. Patten CL, Glick BR. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Appl Environ Microbiol 2002; 68: 3795-3801.
37. Bhardwaj G, Shah R, Joshi B, Patel P. Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004. J App Biol Biotech 2017; 5: 47-52.
38. Benaissa A, Djebbar R, Abderrahmani A. Diversity of plant growth promoting Rhizobacteria of Rhus tripartitus in arid soil of Algeria (Ahaggar) and their physiological properties under abiotic stresses. Adv Hortic Sci 2018; 32: 525-534.
39. Kakar KU, Nawaz Z, Cui Z, Almoneafy AA, Ullah R, Shu Q-Y. Rhizosphere‐associated Alcaligenes and Bacillus strains that induce resistance against blast and sheath blight diseases enhance plant growth and improve mineral content in rice. J Appl Microbiol 2018; 124: 779-796.
40. Miljaković D, Marinković J, Balešević-Tubić S. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms 2020; 8: 1037.
41. Manasa M, Ravinder P, Gopalakrishnan S, Srinivas V, Sayyed RZ, El Enshasy HA, et al. Co-inoculation of Bacillus spp. for growth promotion and iron fortification in sorghum. Sustainability 2021; 13: 12091.
42. Hyder S, Gondal AS, Rizvi ZF, Ahmad R, Alam MM, Hannan A, et al. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Sci Rep 2020; 10: 13859.
43. Liu M, Liu X, Cheng B-S, Ma X-L, Lyu X-T, Zhao X-F, et al. Fang. Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers. Span J Agric Res 2016; 14(4): e1106.
44. Dasgupta D, Sengupta C, Paul G. Screening and identification of best three phosphate solubilizing and IAA producing PGPR inhabiting the rhizosphere of Sesbania bispinosa. Int J Innov Res Sci Eng Technol 2015; 4: 3968-3979.
45. Wan W, Qin Y, Wu H, Zuo W, He H, Tan J, et al. Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil. Front Microbiol 2020; 11: 752.
46. Babar M, Saif-Ur-Rehman, Rasul S, Aslam K, Abbas R, Athar HU, et al. Mining of halo-tolerant plant growth promoting rhizobacteria and their impact on wheat (Triticum aestivum L.) under saline conditions. J King Saud Univ Sci 2021; 33: 101372.
47. Baig KS, Arshad M, Shaharoona B, Khalid A, Ahmed I. Comparative effectiveness of Bacillus spp. possessing either dual or single growth-promoting traits for improving phosphorus uptake, growth and yield of wheat (Triticum aestivum L.). Ann Microbiol 2012; 62: 1109-1119.
48. Lee EY, Hong SH. Plant growth-promoting ability by the newly isolated bacterium Bacillus aerius MH1RS1 from indigenous plant in sand dune. J Korean Soc Environ Eng 2013; 35: 687-693.
49. Abo-Zaid GA, Soliman NAM, Abdullah AS, El-Sharouny EE, Matar SM, Sabry SAF. Maximization of siderophores production from biocontrol agents, Pseudomonas aeruginosa f2 and Pseudomonas fluorescens JY3 using batch and exponential fed-batch fermentation. Processes 2020; 8: 455.
50. Choudhary DK, Johri BN. Interactions of Bacillus spp. and plants - with special reference to induced systemic resistance (ISR). Microbiol Res 2009; 164: 493-513.
51. Marques APGC, Pires C, Moreira H, Rangel AOSS, Castro PML. Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biol Biochem 2010; 42: 1229-1235.
52. Swamy MK, Akhtar MS, Sinniah UR. Response of PGPR and AM fungi toward growth and secondary metabolite production in medicinal and aromatic plants. In Plant Soil Microbes, Springer: Cham, Switzerland 2016; pp. 145-168.
53. Guleria S, Walia A, Chauhan A, Shirkot CK. Molecular characterization of alkaline protease of Bacillus amyloliquefaciens SP1 involved in biocontrol of Fusarium oxysporum. Int J Food Microbiol 2016; 232: 134-143.
54. Khan MS, Gao J, Zhang M, Chen X, Moe TS, Du Y, et al. Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii. 3 Biotech 2020; 10: 305.
55. Castaldi S, Masi M, Sautua F, Cimmino A, Isticato R, Carmona M, et al. Pseudomonas fluorescens showing antifungal activity against Macrophomina phaseolina, a severe pathogenic fungus of soybean, produces phenazine as the main active metabolite. Biomolecules 2021; 11: 1728.
56. Kumari P, Bishnoi SK, Chandra S. Assessment of antibiosis potential of Bacillus sp. against the soil-borne fungal pathogen Sclerotium rolfsii Sacc. (Athelia rolfsii (Curzi) Tu & Kimbrough). Egypt J Biol Pest Control 2021; 31: 1-11.
57. Sharf W, Javaid A, Shoaib A, Khan IH. Induction of resistance in chili against Sclerotium rolfsii by plant-growth-promoting rhizobacteria and Anagallis arvensis. Egypt J Biol Pest Control 2021; 31: 1-11.
| Files | ||
| Issue | Vol 15 No 2 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i2.12480 | |
| Keywords | ||
| Plant growth promoting bacteria (PGPR); Bacillus aerius; Bacillus infantis; Alcaligenes faecalis; Antagonistic activity; 16S rRNA sequencing; Biofertilizers | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Chowhan L, Mir M, Sabra M, El-Habbab A, Kumar BK. Plant growth promoting and antagonistic traits of bacteria isolated from forest soil samples. Iran J Microbiol. 2023;15(2):278-289.
