Isolation and molecular identification of biodegrading Mycobacteria from water supplies of Iranian hospitals
Abstract
Molecular isolation of biodegradable mycobacteria from water supplies of Iranian hospitals Background and objectives: Some microorganisms, mainly members of two genera including Pseudomonas and Mycobacterium, were found to be capable of transforming and degrading of polluting agents. We herein report the isolation of a few mycobacteria with the ability to biodegrade organic and inorganic compounds from water supplies of Iranian hospitals.
Materials and methods: The water samples were collected from hospital water supplies. Isolation processes were done according to standard methods. The colonies were subcultured on Löwenstein-Jensen medium to obtain a pure culture. The identification and characterization of the isolates were based on conventional and molecular methods including direct sequence analysis of almost full length of 16S rRNA gene.
Results: The almost complete 16S rRNA gene sequences of the studied strains revealed that the isolates WP16, AW18-1 and AW18-3 were identified as M. fredriksbergense, AW18-2 as M. austroafricanum, AW27-2 as M. obuense and AW27-6 as M. phocaicum. The relationship between our isolates and standard strains of Mycobacterium was supported by a phylogenetic tree of 16S rRNA gene.
Conclusion: In the current study we were able to isolate and characterize six mycobacteria with capability of transforming and degrading polluting agents from Iranian hospital environments. This is indeed the first report on isolation and characterization of mycobacteria with degrading capability of polluting agents from Iranian hospitals. It can be considered as a pioneer study to open up a new horizon in the study of microbial diversity in Iran with an objective-based and applied approach to tackle environmental challenges.
Berekaa MM. Colonization and Microbial degradation of polyisoprene rubber by Nocardioform Actinomycete Nocardia sp. strain-MBR. Biotechnology 2006; 5:234-239.
Zídková L, Szőköl J, Rucká L, Pátek M, Nešvera J.Biodegradation of phenol using recombinant plasmid- carrying Rhodococcus erythropolis strains. Int Biodeter Biodegr 2013; 84: 179-184.
Márquez-Rocha FJ, Olmos-Soto J, Concepción Rosano- Hernández M, Muriel-GarcíaM. Determination of the hydrocarbon-degrading metabolic capabilities of tropical bacterial isolates. Int Biodeter Biodegr 2005;55: 17-23.
Giraud F, Guiraud P, Kadri M, Blake G, Steiman R.Biodegradation of anthracene and fluoranthene by fungi isolated from an experimental constructed wet land for waste water treatment. Water Res 2001; 35:4126-4136.
Madsen EL. Determining in situ biodegradation: Facts and challenges. Environ Sci Technol 1991; 25: 1663-1673.
Margesin R, Schinner F. Biodegradation of diesel oil by cold-adapted microorganism in presence of sodium dodecyl sulfate. Chemosphere 1999; 38: 3463-3472.
Borde X, Guieysse B, Delgado O, Muñoz R, Hatti-Kaul R, Nugier-Chauvin C, et al. Synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants. Bioresour Technol 2003; 86: 293-300.
Thomson R, Carter R, Gilpin C, Coulter C, Hargreaves M. Comparison of methods for processing drinking water samples for the isolation of Mycobacterium avium and Mycobacterium intracellulare. Appl Environ Microbiol 2008; 74: 3094-3098.
Kent PT, Kubica GP (1985). Public health mycobacteriology: a guide for the level III laboratory. Atlanta, Ga. U.S. Dept. of Health and Human Services, Public Health Service, Centers for Disease Control.
Pitcher DG, Saunders NA, Owen R J. “Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Letters Appl Micro 1989; 8: 151-156.
Khan IU, Yadav JS. Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis. J Clin Microbiol 2004; 42: 453-457.
Shojaei H, Heidarieh P, Hashemi A, Feizabadi MM, Daei Naser. A. Species identification of neglected nontuberculous mycobacteria in a developing country. Jpn J Infect Dis 2011; 64: 265-271.
Jeon YS, Chung H, Park S, Hur I, Lee JH, Chun J. jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 2005; 15; 21: 3171-3173.
Stackebrandt E, Smida J (1988). The phylogeny of the genus Mycobacterium as determined by 16S rRNA sequences and development of DNA probes. Biology of Actinomycetes. Scientific Societies Press, Tokyo, Japan, pp: 244–250.
Mastrangelo G, Fadda E, Marzia V. Polycyclic aromatic hydrocarbons and cancer in man. Environ Health Persp 1996; 104: 1166-1170.
Oliva A, Spira A, Multigner L. Contribution of environmental factors to the risk of male infertility. Hum Reprod 2001; 16: 1768-1776.
Crawford DL, Goodfellow M. Mordarski WM. (Ed). (1988). Biodegradation of agricultural and urban wastes. Actinomycetes in biotechnology. Academic Press, Ltd, London, UK, pp: 433-439.
Stackebrandt E, Fred AR, Ward-Rainey NL.Proposal for a new hierarchic classification system,Actinobacteria classis nov. Int J Syst Bacteriol 1997;47: 479-491.
Kämpfer P, Andersson MA, Rainey FA, Kroppenstedt RM, Salkinoja-Salonen M. Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children’s day care centre. Int J Syst Bacteriol 1999;49: 681-687.
Bell KS, Philp JC, Aw DW, Christofi N. The genus Rhodococcus. J Appl Microbiol 1998; 85: 195-210.
Kleespies M, Kroppenstedt RM, Rainey FA, Webb LE, Stackebrandt E. Mycobacterium hodleri sp. nov., a new member of the fast-growing mycobacteria capable of degrading polycyclic aromatic hydrocarbons. Int J Syst Bacteriol 1996; 46: 683-687.
Häggblom MM, Nohynek LJ, Palleroni NJ, Kronqvist K, Nurmiaho-Lassila EL, Salkinoja-Salonen MS, et al. Transfer of polychlorophenol-degrading Rhodococcus chlorophenolicus (Apajalahti et al. 1986) to the genus Mycobacterium as Mycobacterium chlorophenolicum comb. nov. Int J Syst Bacteriol 1994; 44: 485-493.
Willumsen PA & Karlson U. Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation 1997; 7: 415-423.
Kummer C, Schumann P, Stackebrandt E. Gordonia alkanivorans sp. nov., isolated from tar-contaminated soil. Int J Syst Bacteriol 1999; 49: 1513-1522.
Das K & Mukherjee AK. Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresource Technol 2007; 98: 1339-1345.
Bock C, Kroppenstedt RM, Schmidt U, Diekmann H.Degradation of prochloraz and 2,4,6-trichlorophenol by environmental bacterial strains. Appl Microbiol Biotechnol 1996; 45(1-2): 257-262.
Willumsen P, Karlson U, Stackebrandt E, Kroppenstedt RM. Mycobacterium frederiksbergense sp. nov., a novel polycyclic aromatic hydrocarbon-degrading Mycobacterium species. Int J Syst Evol Microbiol 2001; 51: 1715-1722.
Solano-Serena F, Marchal R, Heiss S, Vandecasteele JP. Degradation of isooctane by Mycobacterium austroafricanum IFP 2173: growth and catabolic pathway. J Appl Microbiol 2004; 97: 629-639.
Tsukamura M, Mizuno S. Mycobacterium obuense, a rapidly growing scotochromogenic mycobacterium capable of forming a black product from p-aminosalicylate and salicylate. J Gen Microbiol 1971; 68: 129-134.
Satsuma K, Masuda M. Reductive dechlorination of methoxychlor by bacterial species of environmental origin: evidence for primary biodegradation of methoxychlor in submerged environments. J Agric Food Chem 2012; 29: 60: 2018-2023.
Adékambi T, Berger P, Raoult D, Drancourt M. rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol 2006; 56: 133-143.
Chen L, Cai T, Wang Q. Characterization of fluoroglycofen ethyl degradation by strain Mycobacterium phocaicum MBWY-1. Curr Microbiol 2011; 62: 1710-1717.
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| Issue | Vol 6 No 4 (2014) | |
| Section | Articles | |
| Keywords | ||
| 16SrRNA gene Biodegradation Mycobacterium water supply | ||
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