Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water
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Abstract
Background and Objectives: Heavy metals pollution is one of the most important concerns in the world. Selenium is one of the most important elements for the life, but if the absorption of this element in cells increases, it acts as a toxic element.
Materials and Methods: In this study, bacterial isolates were screened and isolated from selenium-contaminated soil and water. Twenty -five out of 42 isolates were able to reduce Selenite. Also, the response surface method (RSM) was used to evaluate and optimize the biological reduction of selenite by Selena 3. Factors of bacterial inoculation percentage, time, and amount of selenium oxyanion salt concentration were studied at five levels of -α, -1, 0, +1, and +α.
Results: Bacillus sp. Selena 3 was able to reduce 80 mM sodium selenite in less than 4 hours compared to other bacterial isolates. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sodium selenite Bacillus sp. Selena 3 was reported as 160 and 320 mM, respectively. The results showed that with increasing duration, the percentage of selenite reduction by bacteria increases and the percentage of bacterial inoculation does not have much effect on its reduction.
Conclusion: Due to the ability of Bacillus sp. Selena 3 for rapid reduction in significant concentration of selenium oxyanion (SeO32-), this bacterium can be used as an efficient candidate in removing selenite from the environment.
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25. Mohapatra RK, Parhi PK, Pandey S, Bindhani BK, Thatoi H, Panda CR. Active and passive biosorption of Pb(II)using live and dead biomass of marine bacterium Bacillus xiamenensis PbRPSD202: Kinetics and isotherm studies. J Environ Manage 2019; 247: 121-134.
26. Kessi J, Ramuz M, Wehrli E, Spycher M, Bachofen R. Reduction of Selenite and detoxification of elemental Selenium by the phototrophic bacterium Rhodospirillum rubrum. Appl Environ Microbiol 1999; 65: 4734- 4740.
2. Han W, Mao Y, Wei Y, Shang P, Zhou X. Bioremediation of aquaculture wastewater with algal-bacterial biofilm combined with the production of selenium rich biofertilizer. Water 2020; 12: 2071.
3. Zhang J, Wang Y, Shao Z, Li J, Zan S, Zhou S, et al. Two selenium tolerant Lysinibacillus sp. strains are capable of reducing selenite to elemental Se efficiently under aerobic conditions. J Environ Sci (China) 2019; 77: 238-249.
4. Zheng S, Su J, Wang L, Yao R, Wang D, Deng Y, et al. Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil. BMC Microbiol 2014; 14: 204.
5. Das S, Dash HR, Chakraborty J. Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants. Appl Microbiol Biotechnol 2016; 100: 2967-2984.
6. Lenz M, Lens PN. The essential toxin: the changing perception of selenium in environmental sciences. Sci Total Environ 2009; 407: 3620-3633.
7. Sun H-J, Rathinasabapathi B, Wu B, Luo J, Pu L-P, Ma LQ. Arsenic and selenium toxicity and their interactive effects in humans. Environ Int 2014; 69: 148-158.
8. Tan Y, Yao R, Wang R, Wang D, Wang G, Zheng S. Reduction of selenite to Se(0) nanoparticles by filamentous bacterium Streptomyces sp. ES2-5 isolated from a selenium mining soil. Microb Cell Fact 2016; 15: 157.
9. Lavado R, Shi D, Schlenk D. Effects of salinity on the toxicity and biotransformation of L-selenomethionine in Japanese medaka (Oryzias latipes) embryos: mechanisms of oxidative stress. Aquat Toxicol 2012; 108: 18-22.
10. Herrero Latorre C, Barciela García J, García Martín S, Peña Crecente RM. Solid phase extraction for the speciation and preconcentration of inorganic selenium in water samples: a review. Anal Chim Acta 2013; 804: 37-49.
11. Nancharaiah YV, Lens PN. Ecology and biotechnology of selenium-respiring bacteria. Microbiol Mol Biol Rev 2015; 79: 61-80.
12. Samant S, Naik M, Parulekar K, Charya L, Vaigankar D. Selenium reducing Citrobacter fruendii strain KP6 from Mandovi estuary and its potential application in selenium nanoparticle synthesis. Proc Natl Acad Sci India Sect B: Biol Sci 2016; 88: 10.1007/s40011-016-0815-y.
13. Zhang X, Fan W-Y, Yao M-C, Yang C-W, Sheng G-P. Redox state of microbial extracellular polymeric substances regulates reduction of selenite to elemental selenium accompanying with enhancing microbial detoxification in aquatic environments. Water Res 2020; 172: 115538.
14. Ben-David A, Davidson CE. Estimation method for serial dilution experiments. J Microbiol Methods 2014; 107: 214-221.
15. Brenner DJ, Krieg NR, Staley JT, Garrity GM (2005). Bergey's Manual® of Systematic Bacteriology: The Proteobacteria (Part C). 2nd ed. Springer
16. Tugarova AV, Mamchenkova PV, Khanadeev VA, Kamnev AA. Selenite reduction by the rhizobacterium Azospirillum brasilense, synthesis of extracellular selenium nanoparticles and their characterisation. N Biotechnol 2020; 58: 17-24.
17. Santos S, Ungureanu G, Boaventura R, Botelho C. Selenium contaminated waters: An overview of analytical methods, treatment options and recent advances in sorption methods. Sci Total Environ 2015; 521-522: 246-460.
18. Turanov AA, Xu X-M, Carlson BA, Yoo M-H, Gladyshev VN, Hatfield DL. Biosynthesis of selenocysteine, the 21st amino acid in the genetic code, and a novel pathway for cysteine biosynthesis. Adv Nutr 2011; 2: 122-128.
19. Xu X-M, Carlson BA, Mix H, Zhang Y, Saira K, Glass RS, et al. Biosynthesis of selenocysteine on its tRNA in eukaryotes. PLoS Biol 2007; 5(1): e4.
20. Nancharaiah YV, Venugopalan VP. Denitrification of synthetic concentrated nitrate wastes by aerobic granular sludge under anoxic conditions. Chemosphere 2011; 85: 683-688.
21. He Y, Xiang Y, Zhou Y, Yang Y, Zhang J, Huang H, et al. Selenium contamination, consequences and remediation techniques in water and soils: A review. Environ Res 2018; 164: 288-301.
22. Wadgaonkar SL, Nancharaiah YV, Esposito G, Lens PNL. Environmental impact and bioremediation of seleniferous soils and sediments. Crit Rev Biotechnol 2018; 38: 941-956.
23. Stolz JF, Basu P, Santini JM, Oremland RS. Arsenic and selenium in microbial metabolism. Annu Rev Microbiol 2006; 60: 107-130.
24. Soudi MR, Ghazvini PTM, Khajeh K, Gharavi S. Bioprocessing of seleno-oxyanions and tellurite in a novel Bacillus sp. strain STG-83: A solution to removal of toxic oxyanions in presence of nitrate. J Hazard Mater 2009; 165: 71-77.
25. Mohapatra RK, Parhi PK, Pandey S, Bindhani BK, Thatoi H, Panda CR. Active and passive biosorption of Pb(II)using live and dead biomass of marine bacterium Bacillus xiamenensis PbRPSD202: Kinetics and isotherm studies. J Environ Manage 2019; 247: 121-134.
26. Kessi J, Ramuz M, Wehrli E, Spycher M, Bachofen R. Reduction of Selenite and detoxification of elemental Selenium by the phototrophic bacterium Rhodospirillum rubrum. Appl Environ Microbiol 1999; 65: 4734- 4740.
| Files | ||
| Issue | Vol 15 No 2 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i2.12481 | |
| Keywords | ||
| Bacillus; Metalloids pollution; Selenite bioremediation | ||
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How to Cite
1.
Tavafi H, Tajer-Mohammad-Ghazvini P, Babaei A. Screening, identification and experimental design to optimization of the selenite bioremediation by new isolated Bacillus sp. Selena 3 in water. Iran J Microbiol. 2023;15(2):290-302.
