Phylogenetic characterization of rhinovirus and adenovirus in hospitalized children aged ≤ 18 years with severe acute respiratory infection in Iran
-
Simin Abbasi
,
Nazanin Zahra Shafiei-Jandaghi
,
Azadeh Shadab
,
Seyed Abbas Hassani
,
Abbas Rahimi Foroushani
,
Nazanin Hosseinkhan
,
Fahimeh Aghamir
,
Talat Mokhtari-Azad
,
Jila Yavarian
Abstract
Background and Objectives: Human rhinoviruses (HRVs) and human adenoviruses (HAdVs) are among the most prevalent viruses in hospitalized patients with severe acute respiratory infection (SARI). This study aimed to evaluate the molecular characterization of HRV and HAdV in hospitalized patients with SARI, who aged ≤ 18 years in Tehran, Iran.
Materials and Methods: To detect these two viruses, a conventional nested RT-PCR (Reverse transcription-polymerase chain reaction) assay was performed on 264 throat swabs collected from December 2018 to March 2019. The epidemiological data were analyzed and phylogenetic trees were constructed.
Results: Of 264 cases with SARI, 36 (13.6%) and 28 (10.6%) were positive for HAdV and HRV respectively. Of 21 HRV sequenced samples, HRV-A (42.9%), HRV-B (9.5%) and HRV-C (47.6%) and of 36 HAdV sequenced samples, HAdV-C6 (38.9%), HAdV-B7 (22.2%), HAdV-B3 (11.1%), HAdV-B16 (5.6%), HAdV-C5 (13.9%), HAdV-C57 (5.6%), HAdV-E4 (2.8%); were detected in children with SARI. Some viral genotypes appeared to cause more severe disease, which may lead to hospitalization.
Conclusion: Large-scale studies are recommended to investigate the epidemiology and molecular characterizations through surveillance networks to provide useful information on etiology, seasonality, and demographic associations in patients with SARI.
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24. Dhingra A, Hage E, Ganzenmueller T, Böttcher S, Hofmann J, Hamprecht K, et al. Molecular evolution of human adenovirus (HAdV) species C. Sci Rep 2019; 9: 1039.
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26. Ahn JG, Kim DS, Kim KH. Clinical characteristics and cytokine profiles of children with acute lower respiratory tract infections caused by human rhinovirus. PLoS One 2018; 13(7): e0198624.
27. Haddad-Boubaker S, Mefteh K, Mejri C, Bouaffsoun A, El Moussi A, Boutiba I, et al. High genotypic diversity of Rhinoviruses obtained from Tunisian children with severe acute respiratory infection. J Infect Dev Ctries 2021; 15: 726-735.
28. Su Y-T, Lin Y-T, Yang C-C, Tsai S-S, Wang J-Y, Huang Y-L, et al. High correlation between human rhinovirus type C and children with asthma exacerbations in Taiwan. J Microbiol Immunol Infect 2020; 53: 561-568.
29. Zlateva KT, Van Rijn AL, Simmonds P, Coenjaerts FEJ, Van Loon AM, Verheij TJM, et al. Molecular epidemiology and clinical impact of rhinovirus infections in adults during three epidemic seasons in 11 European countries (2007–2010). Thorax 2020; 75: 882-890.
30. Li W, Yu B, Zhou J, Wang Y, Xue B, Pan J, et al. Genetic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Guangzhou, China. Virol J 2021; 18: 174.
2. Yao L-H, Wang C, Wei T-L, Wang H, Ma F-L, Zheng L-S. Human adenovirus among hospitalized children with respiratory tract infections in Beijing, China, 2017–2018. Virol J 2019; 16: 78.
3. Li Z-J, Zhang H-Y, Ren L-L, Lu Q-B, Ren X, Zhang C-H, et al. Etiological and epidemiological features of acute respiratory infections in China. Nat Commun 2021; 12: 5026.
4. Zadheidar S, Yavarian J, Heydarifard Z, Nejati A, Sadeghi K, Ghavami N, et al. Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study. BMC Pediatr 2022; 22: 583.
5. Gandhi L, Maisnam D, Rathore D, Chauhan P, Bonagiri A, Venkataramana M. Respiratory illness virus infections with special emphasis on COVID-19. Eur J Med Res 2022; 27: 236.
6. Dou Y, Li Y, Ma C, Zhu H, Du J, Liu H, et al. Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction. Mol Med Rep 2018; 18: 2889-2897.
7. Khalaf RJ, Fadhil HY, Aufi IM, Namdar SA. Molecular diagnosis of human adenovirus in children with upper respiratory tract infections. J Pharm Biol Sci 2017; 12: 09-13.
8. Lu G, Peng X, Li R, Liu Y, Wu Z, Wang X, et al. An outbreak of acute respiratory infection at a training base in Beijing, China due to human adenovirus type B55. BMC Infect Dis 2020; 20: 537.
9. Lam WY, Yeung ACM, Tang JW, Ip M, Chan EWC, Hui M, et al. Rapid multiplex nested PCR for detection of respiratory viruses. J Clin Microbiol 2007; 45: 3631-3640.
10. Savolainen C, Blomqvist S, Mulders MN, Hovi T. Genetic clustering of all 102 human rhinovirus prototype strains: serotype 87 is close to human enterovirus 70. J Gen Virol 2002; 83: 333-340.
11. Chakhunashvili G, Wagner AL, Power LE, Janusz CB, Machablishvili A, Karseladze I, et al. Severe Acute Respiratory Infection (SARI) sentinel surveillance in the country of Georgia, 2015-2017. PLoS One 2018; 13(7): e0201497.
12. Pscheidt VM, Gregianini TS, Martins LG, Veiga ABGd. Epidemiology of human adenovirus associated with respiratory infection in southern Brazil. Rev Med Virol 2021; 31(4): e2189.
13. Malekshahi SS, Azad TM, Yavarian J, Shahmahmoodi S, Naseri M, Rezaei F. Molecular detection of respiratory viruses in clinical specimens from children with acute respiratory disease in Iran. Pediatr Infect Dis J 2010; 29: 931-933.
14. Hatem A, Mohamed S, Abu Elhassan UE, Ismael EAM, Rizk MS, El-Kholy A, et al. Clinical characteristics and outcomes of patients with severe acute respiratory infections (SARI): results from the Egyptian surveillance study 2010–2014. Multidiscip Respir Med 2019; 14: 11.
15. Leotte J, Trombetta H, Faggion HZ, Almeida BM, Nogueira MB, Vidal LR, et al. Impact and seasonality of human rhinovirus infection in hospitalized patients for two consecutive years. J Pediatr (Rio J) 2017; 93: 294-300.
16. Rady HI, El Kholy A. Prevalence of Human rhinovirus infection in young children with acute wheezing. Gaz Egypt Paediatr Assoc 2018; 66: 35-38.
17. Essa S, Owayed A, Altawalah H, Khadadah M, Behbehani N, Al-Nakib W. Mixed viral infections circulating in hospitalized patients with respiratory tract infections in kuwait. Adv Virol 2015; 2015: 714062.
18. Zhao M-C, Guo Y-H, Qiu F-Z, Wang L, Yang S, Feng Z-S, et al. Molecular and clinical characterization of human adenovirus associated with acute respiratory tract infection in hospitalized children. J Clin Virol 2020; 123: 104254.
19. Nakamura H, Fujisawa T, Suga S, Taniguchi K, Nagao M, Ito M, et al. Species differences in circulation and inflammatory responses in children with common respiratory adenovirus infections. J Med Virol 2018; 90: 873-880.
20. Bautista-Gogel J, Madsen CM, Lu X, Sakthivel SK, Froh I, Kamau E, et al. Outbreak of Respiratory Illness Associated With Human Adenovirus Type 7 Among Persons Attending Officer Candidates School, Quantico, Virginia, 2017. J Infect Dis 2020; 221: 697-700.
21. Haque E, Banik U, Monwar T, Anthony L, Adhikary AK. Worldwide increased prevalence of human adenovirus type 3 (HAdV-3) respiratory infections is well correlated with heterogeneous hypervariable regions (HVRs) of hexon. PLoS One 2018; 13(3): e0194516.
22. Wu X, Lu X, Schneider E, Ahmed JA, Njenga MK, Breiman RF, et al. Reassessment of high prevalence human adenovirus detections among residents of two refugee centers in Kenya under surveillance for acute respiratory infections. J Med Virol 2019; 91: 385-391.
23. Coleman KK, Robie ER, Abdelgadir A, Kozhumam AS, Binder RA, Gray GC. Six decades of human adenovirus type 4 infections reviewed: increasing infections among civilians are a matter of concern. Clin Infect Dis 2021; 73: 740-746.
24. Dhingra A, Hage E, Ganzenmueller T, Böttcher S, Hofmann J, Hamprecht K, et al. Molecular evolution of human adenovirus (HAdV) species C. Sci Rep 2019; 9: 1039.
25. Pomeranz G, Pando R, Hindiyeh M, Sherbany H, Meningher T, Sharabi S, et al. Rhinovirus infections in infants suggest that early detection can prevent unnecessary treatment. J Clin Virol 2019; 115: 11-17.
26. Ahn JG, Kim DS, Kim KH. Clinical characteristics and cytokine profiles of children with acute lower respiratory tract infections caused by human rhinovirus. PLoS One 2018; 13(7): e0198624.
27. Haddad-Boubaker S, Mefteh K, Mejri C, Bouaffsoun A, El Moussi A, Boutiba I, et al. High genotypic diversity of Rhinoviruses obtained from Tunisian children with severe acute respiratory infection. J Infect Dev Ctries 2021; 15: 726-735.
28. Su Y-T, Lin Y-T, Yang C-C, Tsai S-S, Wang J-Y, Huang Y-L, et al. High correlation between human rhinovirus type C and children with asthma exacerbations in Taiwan. J Microbiol Immunol Infect 2020; 53: 561-568.
29. Zlateva KT, Van Rijn AL, Simmonds P, Coenjaerts FEJ, Van Loon AM, Verheij TJM, et al. Molecular epidemiology and clinical impact of rhinovirus infections in adults during three epidemic seasons in 11 European countries (2007–2010). Thorax 2020; 75: 882-890.
30. Li W, Yu B, Zhou J, Wang Y, Xue B, Pan J, et al. Genetic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Guangzhou, China. Virol J 2021; 18: 174.
| Files | ||
| Issue | Vol 15 No 1 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i1.11932 | |
| Keywords | ||
| Human rhinovirus; Human adenovirus; Respiratory infection; Phylogeny; Iran | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Abbasi S, Shafiei-Jandaghi NZ, Shadab A, Hassani SA, Rahimi Foroushani A, Hosseinkhan N, Aghamir F, Mokhtari-Azad T, Yavarian J. Phylogenetic characterization of rhinovirus and adenovirus in hospitalized children aged ≤ 18 years with severe acute respiratory infection in Iran. Iran J Microbiol. 2023;15(1):155-162.
