Fermentative production of carotenoids from marine actinomycetes


Background and Objectives: In marine actinomycetes, carotenoid production occurs in constitutive, light-dependent or cryptic manner. The present work deals with the fermentative production of carotenoids from marine actinomycetes.
Materials and Methods: Marine actinomycetes namely Streptomyces strain AQBMM35 was isolated from the marine sponge Mycale mytilorum collected from South West coast of India using ISP media. The Streptomyces isolates were characterized for their colony characteristics, morphological properties, physiological and biochemical properties and were tentatively identified. Fermentation of the strain under fluorescent white light was carried out for the production of carotenoids. UV spectrum, TLC and HPLC analysis were done for the confirmation of carotenoids.
Results: The characteristics studied strongly suggest that the strain AQBMM35 belongs to the genus Streptomyces sp. It has been found that Streptomyces strain (AQBMM35) fermenting under fluorescent white light produced carotenoids. Spectrophotometric analysis of the carotenoid fraction revealed a peak at 280 nm. TLC analysis of the carotenoid extract showed the presence of phytoene (Rf of 0.81). HPLC confirmed the production of phytoene when compared with standards.
Conclusion: The fermenting sponge-associated Streptomyces isolate (AQBMM35) produced carotenoids namely phytoene. If this symbiotic Streptomyces strain, from which secondary metabolite like carotenoids are derived, can be cultured under light, then it can be used for mass production of precursor pigment and it can be used as an antioxidant and also as a food additive.

Sandmann G. Carotenoid biosynthesis and biotechno- logical application. Arch Biochem Biophys 2001; 385:4-12.

Chattopadhyay P, Chatterjee S, Sen SK. Biotechnological potential of natural food grade biocolorants. African Journal of Biotechnology 2008; 7 (17): 2972-2985.

Takano H, Asker D, Beppu T, Ueda K. Genetic control for light-induced carotenoid production in non- phototrophic bacteria. J Ind Microbiol Biotechnol 2006;33: 88-93.

Dharmaraj S, Ashokkumar B, Dhevendaran K.Food-grade pigments from Streptomyces sp. isolated from the marine sponge Callyspongia diffusa. Food Res Int 2009; 42: 487-492.

Hooper JNA and Van Soest RWM (2002). Systema Porifera: a guide to the classification of sponges. Kluwer Academic/Plenum Publishers, New York.

Montalvo NF, Mohamed NM, Enticknap JJ, Hill RT.Novel actinobacteria from marine sponges. Antonie van Leeuwenhoek 2005; 87: 29-36.

Shirling EB and Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313-340.

Buchanan RE and Gibbons NE. Bergey’s manual of determinative bacteriology, 7th edn, London. Bergey Taxon 1974; 24: 377-378.

Xu LH and Lee WJ (2006). Actinomycete systematic- prin- ciple, methods and practice. Science, Beijing, pp. 381-387.

Okazaki T and Okami Y. Actinomycetes tolerant to increased sodium chloride concentration and their metabolites. J Fermentation Technol 1975; 53: 833-840.

Locci R (1989) Streptomycetes and related genera. In: Williams ST, Sharpe ME, Holt JG, editors. Bergey’s manual of syatematic bacteriology, pp. 2451-2493. Wil- liams and Wilkins, Baltimore.

Schumann G, Nurnberger H, Sandmann G, Krugel H.Activation and analysis of cryptic crt genes for carotenoid biosynthesis from Streptomyces griseus. Mol Gen Genet 1996; 252: 658-666.

Faulkner DJ, Harper MK, Haygood MG, Salomon CE, Schmidt EW (2000). Symbiotic bacteria in sponges: sources of bioactive substances, pp. 107-119. In N. Fusetani (ed.), Drugs from the sea. Karger, Basel, Switzerland.

Dharmaraj S and Alagarsamy S. Bioactive potential of Streptomyces isolated from marine sponges, World J Microbiol Biotechnol 2009; 25: 1971-1979.

Ernst H. Recent advances in industrial carotenoid synthesis. Pure Appl Chem 2002; 74: 1369-1382.

IssueVol 1 No 4 (2009) QRcode
Marine actinomycetes Streptomyces Fermentation Carotenoids Phytoene

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How to Cite
Dharmaraj S, Ashokkumar B, Dhevendaran K. Fermentative production of carotenoids from marine actinomycetes. Iran J Microbiol. 1;1(4):36-41.