Articles

Role of Gardnerella vaginalis as an etiological agent of bacterial vaginosis

Abstract

Background and Objectives: Bacterial vaginosis is a risk factor for obstetric infections, various adverse outcomes of pregnancy and pelvic inflammatory disease. The objectives of this study were to assess the prevalence of bacterial vaginosis in women attending Gynaecology Outpatient Department (O.P.D) and sexually transmitted disease (S.T.D.) clinic and to assess the role of Gardnerella vaginalis as an etiological agent of bacterial vaginosis.
Materials and Methods: Two hundred women attending Gynaecology O.P.D and S.T.D. clinic with symptoms suggesting lower genital tract infection were included in the study. pH of the vaginal discharge was measured and three high vaginal swabs were collected. Bacterial vaginosis was diagnosed using Amsel’s criteria and Nugent’s method. Gardnerella vaginalis was isolated and identified by standard methods.
Results: Prevalence of bacterial vaginosis using Amsel’s criteria and Gram stain scoring method was found to be 51.5%and 49% respectively. Gardnerella vaginalis was isolated in only 8.7% cases of bacterial vaginosis.
Conclusion: Our study showed a relatively high prevalence of bacterial vaginosis in the population under study. Women attending various healthcare facilities should be screened and treated properly to prevent recurrence. Low isolation rate of Gardnerella vaginalis may be attributed to factors like poor viability and fastidiousness of the organism to grow in various media.

Hill GB. The microbiology of bacterial vaginosis. Am J Obstet Gynecol 1993; 169:450-454.

Sobel Jack D. Bacterial Vaginosis. Annu Rev Med 2000; 51: 349-356.

Saharan SP, Surve C, Raut V, Bhattacharya M.Diagnosis and prevalence of bacterial vaginosis. J Postgrad Med 1993; 39: 72-73.

Pheifer TA, Forsyth PS, Durfee MA, Pollock HM, Holmes KK. Nonspecific vaginitis: role of Haemophilus vaginalis and treatment with metronidazole. N Engl J Med 1978; 298:1429-1434.

Bramley HM, Dixon RA, Jones BM. Haemophilus vaginalis (Corynebacterium vaginale, Gardnerella vaginalis) in a family planning clinic population. Br J Vener Dis 1981; 57: 62-66.

Bhalla P, Chawla R, Garg S, Singh MM, Raina U, Bhalla R, et al. P. Prevalence of bacterial vaginosis among women in Delhi,India. Indian J Med Res 2007;125: 167-172.

Amsel R, Totten PA, Spiegel CA, Eschenbach DA, Holmes KK. Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med 1983; 74:14-22.

Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardised method of Gram stain interpretation. J Clin Microbiol 1991; 29: 297-301.

Rao PS, Devi S, Shriyan A, Rajaram M, Chandra KJ.Diagnosis of bacterial vaginosis in a rural set up:Comparison of clinical algorithm, smear scoring and culture by semiquantitative technique. Indian J Med Microbiol 2004; 22:47-50.

Howard AJ, Ison Catherine A. Haemophilus, Gardnerella and other bacilli. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th ed. Edinburgh: Churchill Livingstone; 1996. P.449-64.

Bhujwala RA, Buckshee K, Shriniwas. Gardnerella vaginalis and associated aerobic bacteria in non- specific vaginitis. Indian J Med Res 81,1985; 251-256.

Eschenbach DA. Bacterial vaginosis and anaerobes in obstetric-gynecologic infection. Clin Infect Dis 1993;16:282-287.

Joesoef MR, Wiknjosastro G, Norojono W, Sumampouw H, Linnan M, Hansell MJ, et al. Coinfection with chlamydia and gonorrhoea among pregnant women with bacterial vaginosis. Int J STD AIDS 1996; 7: 61-64.

Bhalla P, Kaushika A. Epidemiological and microbiological correlates of bacterial vaginosis. Indian J Dermatol Venereol Leprol 1994; 60 :8-14.

Mohadani JW, Dekate RR, Shrikhande AV.Cytodiagnosis of discharge per vaginum. Indian J Pathol Microbiol 1998; 41:403-411.

Hay PE. Recurrent bacterial vaginosis. Dermatol Clin 1998; 16:769-773.

Chandeying V, Skov S, Kemapunmanus M, Law M, Geater A, Rowe P. Evaluation of two clinical protocols for the management of women with vaginal discharge in Southern Thailand. Sex Transm Infect 1998; 74:194-201.

Rosenstein IJ, Morgan DJ, Sheehan M, Lamont RF, Taylor RD. Bacterial vaginosis in pregnancy: distribution of bacterial species in different Gram- stain cetegories of the vaginal flora. J Med Microbiol 1996; 45: 120-126.

Esim BE, Kars B, Karsidag AY, Karadeniz BI, Kaymaz O, Gencer S, et al. Diagnosis of vulvovaginitis: comparison of clinical and microbiological diagnosis. Arch Gynecol and Obstet 2010; 282:515-519.

Greenwood JR, Pickett MJ. Salient features of Haemophilus vaginalis. J Clin Microbiol 1979; 9: 200-204.

Files
IssueVol 6 No 6 (2014) QRcode
SectionArticles
Keywords
Bacterial vaginosis Gardnerella vaginalis Gynaecology

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Baruah FK, Sharma A, Das C, Hazarika N, Hussain JH. Role of Gardnerella vaginalis as an etiological agent of bacterial vaginosis. Iran J Microbiol. 1;6(6):409-414.