Original Article

Isolation of fecal Escherichia coli from fresh strawberries (fragaria ananassa) in Iran

Abstract

Background and Objectives: Microbial safety of the fresh fruits is a global concern. The production of strawberry (Fragaria ananassa) in Iran has grown 4.5% from 2016 to 2019. Little information is available about the microbiological quality of strawberry produced in Iran. The objective of this investigation was to assess the fecal Escherichia coli (FEC) contamination of fresh strawberries of south of Kerman province where is the centers of production and supply of strawberries to other parts of Iran.
Materials and Methods: In this cross-sectional descriptive study, the FEC of a total number of 109 strawberry samples, which were collected from green-houses of strawberry of Kerman province during three months, were enumerated using the most probable number (MPN) as described by Iranian National Standards Organization, protocol No.2946, and interpreted using the latest statute released by Iran Veterinary Organization, Executive order number 2946, released in 2011.
Results: MPN of FEC counted in strawberries ranged between <0.3 (n=37) and >110 (n=19) per gram (g) having a mean, mode, and median value of 250.3, <0.3, and 9.4 MPN/g, respectively. More than one-fourth of the samples (28.44%) were polluted with FEC at a level of >100 MPN/g.
Conclusion: Our findings may be resulted from the sanitary quality of the farm and strawberries of the study area, which indicated that the microbial safety of the strawberries in this survey was not satisfactory, alarming public health.

1. Amrutha B, Sundar K, Shetty PH. Study on E. coli and Salmonella biofilms from fresh fruits and vegetables. J Food Sci Technol 2017; 54: 1091-1097.
2. Callejón RM, Rodríguez-Naranjo MI, Ubeda C, Hornedo-Ortega R, Garcia-Parrilla MC, Troncoso AM. Reported foodborne outbreaks due to fresh produce in the United States and European Union: Trends and causes. Foodborne Pathog Dis 2015; 12: 32-38.
3. Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, et al. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ Microbiol 2010; 12: 2385-2397.
4. Verma P, Saharan VV, Nimesh S, Singh AP. Phenotypic and virulence traits of Escherichia coli and Salmonella strains isolated from vegetables and fruits from India. J Appl Microbiol 2018; 125: 270-281.
5. Denis N, Zhang H, Leroux A, Trudel R, Bietlot H. Prevalence and trends of bacterial contamination in fresh fruits and vegetables sold at retail in Canada. Food Control 2016; 67: 225-234.
6. Food And Agriculture Organitzation of the United Nations [FAOSTAT] (2019). Available from:
www.fao.org/faostat/en/#compare (accessed 01 September 2019)
7. Health Canada [HC] (2008). Health products and food Branch standards and guidelines for the microbio­logical safety of food – An interpretive summary. Health Canada, Ottawa, ON, Canada. Available from: http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/volume1-eng.php (accessed 01 October 2021)
8. Delbeke S, Ceuppens S, Hessel CT, Castro I, Jacxsens L, De Zutter L, et al. Microbial safety and sanitary quality of strawberry primary production in Belgium: risk factors for Salmonella and Shiga toxin-producing Escherichia coli contamination. Appl Environ Microbiol 2015; 81: 2562-2570.
9. Feng P, Weagant SD, Grant MA, Burkhardt W (2002). Enumeration of Escherichia coli and the Coliform Bacteria. In: Bacteriological Analytical Manual. Washington, USA: United States Food and Drug Administration; Available from: www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm064948.htm (accessed 01 October 2016).
10. United States Department of Agriculture Food Safety And Inspection Service (2014). Most Probable Number from Serial Dilutions. BAM Appendix 2. United States Department of Agriculture Food Safety And Inspection Service, Office of Public Health Science. Available from: [https://www.fda.gov/food/laboratory-methods-food/bam-appendix-2-most-probable-number-serial-dilutions] (accessed 16 September 2022).
11. Zare Jeddi M, Yunesian M, Es’haghi Gorji M, Noori N, Pourmand MR, Jahed Khaniki GR. Microbial evaluation of fresh, minimally-processed vegetables and bagged sprouts from chain supermarkets. J Health Popul Nutr 2014; 32:391-399.
12. Ortiz-Solà J, Viñas I, Colás-Medà P, Anguera M, Abadias M. Occurrence of selected viral and bacterial pathogens and microbiological quality of fresh and frozen strawberries sold in Spain. Int J Food Microbiol 2020; 314: 108392.
13. Mukherjee A, Speh D, Dyck E, Diez-Gonzalez F. Preharvest evaluation of coliforms, Escherichia coli, Salmonella, and Escherichia coli O157:H7 in organic and conventional produce grown by Minnesota farmers. J Food Prot 2004; 67: 894-900.
14. Bohaychuk VM, Bradbury RW, Dimock R, Fehr M, Gensler GE, King RK, et al. A microbiological survey of selected Alberta-grown fresh produce from farmers’ markets in Alberta, Canada. J Food Prot 2009; 72: 415-420.
15. Johannessen GS, Eckner KF, Heiberg N, Monshaugen M, Begum M, Økland M, et al. Occurrence of Escherichia coli, Campylobcter, Salmonella and Shiga-toxin producing E. coli in Norwegian primary strawberry production. Int J Environ Res Public Health 2015; 12: 6919-6932.
16. Barkhori-Mahni M, Rezaeian M, Beigom-Kia E, Raeisi R, Khanaliha Kh, Tarighi F, et al. Prevalence of intestinal parasitic infections in Jiroft, Kerman Province, Iran. Iran J Parasitol 2016; 11: 232-238.
17. Yoon Y, Kim K, Nam M, Shim W-B, Ryu J-G, Kim D-H, et al. Microbiological assessment in strawberry production and recommendations to establish a good agricultural practice system. Foodborne Pathog Dis 2010; 7: 1511-1519.
18. World Health Organization [WHO] (2008). Microbiological Hazards In Fresh Leafy Vegetables And Herbs: Meeting Report. World Health Organization, Geneva. Available from: www.fao.org/3/i0452e/i0452e.pdf (accessed 01 October 2016).
19. Goodburn C, Wallace CA. The microbiological efficacy of decontamination methodologies for fresh produce: A review. Food Control 2013; 32: 418-427.
20. Olaimat AN, Holley RA. Factors influencing the microbial safety of fresh produce: a review. Food Microbiol 2012; 32: 1-19.
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IssueVol 14 No 5 (2022) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijm.v14i5.10963
Keywords
Contamination; Escherichia coli; Fragaria; Fruit; Iran; Public health

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How to Cite
1.
Hamidian N, Salari S, Rashki A, Najimi M. Isolation of fecal Escherichia coli from fresh strawberries (fragaria ananassa) in Iran. Iran J Microbiol. 2022;14(5):683-688.