Investigation of human parvovirus B19 prevalence in a large healthy umbilical cord blood donors
,
Abstract
Background and Objectives: Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is regarded as a potential threat to UCB contamination. This study aimed to evaluate the prevalence of parvovirus B19 in cord blood donors by Semi-Nested PCR. This study is the first large-scale report of the B19 DNA in cord blood donors in Iran.
Materials and Methods: A total of 691 umbilical cord blood were collected under standard procedure. Then, DNA from buffy coat and plasma were extracted, and semi-nested PCR was performed for all samples.
Results: Two out of 691 samples (0.29%) indicated viremia in plasma and buffy coat.
Conclusion: In this line, designing and validating a quantitative PCR assay for detection, quantification, and discrimination of Human B19 DNA genotypes of cord blood donors is necessary to enhance the safety of this source of stem cells.
1. Smith AR, Wagner JE. Alternative haematopoietic stem cell sources for transplantation: place of umbilical cord blood. Br J Haematol 2009;147:246-261.
2. Rogers I, Casper RF. Umbilical cord blood stem cells. Best Pract Res Clin Obstet Gynaecol 2004;18:893-908.
3. Eapen M, Rubinstein P, Zhang MJ, Stevens C, Kurtzberg J, Scaradavou A, et al. Outcomes of transplantation of unrelated donor umbilical cord blood and bone marrow in children with acute leukaemia: a comparison study. Lancet 2007;369:1947-1954.
4. Aghideh AN, Kheirandish M, Abolghasemi H, Gharehbaghian A. Platelet growth factors suppress ex vivo expansion and enhance differentiation of umbilical cord blood CD133+ stem cells to megakaryocyte progenitor cells. Growth Factors 2010;28:409-416.
5. Noroozi-Aghideh A, Kheirandish M. Human cord blood-derived viral pathogens as the potential threats to the hematopoietic stem cell transplantation safety: A mini review. World J Stem Cells 2019;11:73-83.
6. Meissner-Roloff M, Gaggia L, Vermeulen M, Mazanderani AFH, du Plessis NM, Steel HC, et al. Strategies for screening cord blood for a public cord blood bank in high HIV prevalence regions. Glob Health Epidemiol Genom 2018;3:e9.
7. de Pagter PJ, Schuurman R, Visscher H, de Vos M, Bierings M, van Loon AM, et al. Human herpes virus 6 plasma DNA positivity after hematopoietic stem cell transplantation in children: an important risk factor for clinical outcome. Biol Blood Marrow Transplant 2008;14:831-839.
8. Abiodun I, Opaleye OO, Ojurongbe O, Fagbami AH. Seroprevalence of parvovirus B19 IgG and IgM antibodies among pregnant women in Oyo State, Nigeria. J Infect Dev Ctries 2013;7:946-950.
9. Ergaz Z, Ornoy A. Parvovirus B19 in pregnancy. Reprod Toxicol 2006;21:421-435.
10. Aberham C, Pendl C, Gross P, Zerlauth G, Gessner M. A quantitative, internally controlled real-time PCR Assay for the detection of Parvovirus B19 DNA. J Virol Methods 2001;92:183-191.
11. Laub R, Strengers P. Parvoviruses and blood products. Pathol Biol (Paris) 2002;50:339-348.
12. Lehmann HW, von Landenberg P, Modrow S. Parvovirus B19 infection and autoimmune disease. Autoimmun Rev 2003;2:218-223.
13. Giorgio E, De Oronzo MA, Iozza I, Di Natale A, Cianci S, Garofalo G, et al. Parvovirus B19 during pregnancy: a review. J Prenat Med 2010;4:63-66.
14. Hokynar K, Norja P, Laitinen H, Palomaki P, Garbarg-Chenon A, Ranki A, et al. Detection and differentiation of human Parvovirus variants by commercial quantitative real-time PCR tests. J Clin Microbiol 2004;42:2013-2019.
15. Lunardi C, Tinazzi E, Bason C, Dolcino M, Corrocher R, Puccetti A. Human Parvovirus B19 infection and autoimmunity. Autoimmun Rev 2008;8:116-120.
16. Riipinen A, Vaisanen E, Nuutila M, Sallmen M, Karikoski R, Lindbohm ML, et al. Parvovirus b19 infection in fetal deaths. Clin Infect Dis 2008;47:1519-1525.
17. Crane J, Mundle W, Boucoiran I, Maternal Fetal Medicine C. Parvovirus B19 infection in pregnancy. J Obstet Gynaecol Can 2014;36:1107-1116.
18. Bonvicini F, Manaresi E, Gallinella G, Gentilomi GA, Musiani M, Zerbini M. Diagnosis of fetal parvovirus B19 infection: value of virological assays in fetal specimens. BJOG 2009;116:813-817.
19. Shahrokhi S, Ebtekar M, Alimoghaddam K, Pourfathollah AA, Kheirandish M, Ardjmand A, et al. Substance P and calcitonin gene-related neuropeptides as novel growth factors for ex vivo expansion of cord blood CD34(+) hematopoietic stem cells. Growth Factors 2010;28:66-73.
20. Golchin N, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z. Quantification of viral genome in cord blood donors by real time PCR to investigate human Herpesvirus type 8 active infection. Transfus Apher Sci 2015;53:378-380.
21. Abedi E, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z, et al. Quantitative polymerase chain reaction for detection of human Herpesvirus-7 infection in umbilical cord blood donors. Transpl Infect Dis 2015;17:21-24.
22. Abedi E, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z, et al. Quantification of active and latent form of human Cytomegalovirus infection in umbilical cord blood donors by Real-Time PCR. Int J Organ Transplant Med 2017;8:140-145.
23. Marhamati N, Shirvani F, Azimi L, Hashemieh M, Hosseini A, Asad Shahmirzadi S, et al. Molecular detection of human Parvovirus B19 in umbilical cord blood: case study of Tehran, Iran. Gene Reports 2021;23:101183.
24. Norbeck O, Papadogiannakis N, Petersson K, Hirbod T, Broliden K, Tolfvenstam T. Revised clinical presentation of Parvovirus B19-associated intrauterine fetal death. Clin Infect Dis 2002;35:1032-1038.
25. Landolsi H, Yacoubi MT, Bouslama L, Lahmar A, Trabelsi A, Hmissa S, et al. Detection of the human Parvovirus B19 in nonimmune hydrops fetalis using immunohistochemistry and nested-PCR in formalin-fixed and paraffin-embedded placenta and fetal tissues. Pathol Biol (Paris) 2009;57(3):e1-7.
26. Heegaard ED, Brown KE. Human Parvovirus B19. Clin Microbiol Rev 2002;15:485-505.
27. Slavov SN, Rodrigues ES, Sauvage V, Caro V, Diefenbach CF, Zimmermann AM, et al. Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil. J Med Virol 2019;91:1224-1231.
28. Heegaard ED, Petersen BL, Heilmann CJ, Hornsleth A. Prevalence of Parvovirus B19 and Parvovirus V9 DNA and antibodies in paired bone marrow and serum samples from healthy individuals. J Clin Microbiol 2002;40:933-936.
29. Tolfvenstam T, Broliden K. Parvovirus B19 infection. Semin Fetal Neonatal Med 2009;14:218-221.
30. Mendelson E, Aboudy Y, Smetana Z, Tepperberg M, Grossman Z. Laboratory assessment and diagnosis of congenital viral infections: Rubella, Cytomegalovirus (CMV), Varicella-zoster virus (VZV), Herpes Simplex Virus (HSV), Parvovirus B19 and human immunodeficiency virus (HIV). Reprod Toxicol 2006;21:350-382.
31. Zhou Y, Bian G, Zhou Q, Gao Z, Liao P, Liu Y, et al. Detection of cytomegalovirus, human Parvovirus B19, and herpes simplex viru“ 1/2 in women with firs” trimester spontaneous abortions. J Med Virol 2015;87:1749-1753.
32. Tognon M, Tagliapietra A, Magagnoli F, Mazziotta C, Oton-Gonzalez L, Lanzillotti C, et al. Investigation on spontaneous abortion and human Papillomavirus infection. Vaccines (Basel) 2020;8:473.
33. Parsania M, Bamdad T, Hassan ZM, Kheirandish M, Pouriayevali MH, Sari RD, et al. Evaluation of apoptotic and anti-apoptotic genes on efficacy of DNA vaccine encoding glycoprotein B of Herpes Simplex virus type 1. Immunol Lett 2010;128:137-142.
34. Meshkat Z, Soleimanjahi H, Mahmoudi M, Hassan ZM, Mirshahabi H, Meshkat M, et al. CTL responses to a DNA vaccine encoding E7 gene of human Papillomavirus type 16 from an Iranian isolate. Iran J
Immunol 2008;5:82-91.
35. Servey JT, Reamy BV, Hodge J. Clinical presentations of Parvovirus B19 infection. Am Fam Physician 2007;75:373-376.
36. Rollin R, Alvarez-Lafuente R, Marco F, Jover JA, Hernandez-Garcia C, Rodriguez-Navas C, et al. Human Parvovirus B19, Varicella zoster virus, and human Herpesvirus-6 in mesenchymal stem cells of patients with osteoarthritis: analysis with quantitative real-time polymerase chain reaction. Osteoarthritis Cartilage 2007;15:475-478.
37. Teixeira LO, Amaral SC, Finger-Jardim F, da Hora VP, Goncalves CV, Soares MA, et al. [Frequency of human Papillomavirus in the placenta, in the colostrum and in the umbilical cord blood]. Rev Bras Ginecol Obstet 2015;37:203-207.
38. Koskimaa HM, Waterboer T, Pawlita M, Grenman S, Syrjanen K, Syrjanen S. Human Papillomavirus genotypes present in the oral mucosa of newborns and their concordance with maternal cervical human papillomavirus genotypes. J Pediatr 2012;160:837-843.
39. Worda C, Huber A, Hudelist G, Schatten C, Leipold H, Czerwenka K, et al. Prevalence of cervical and intrauterine human Papillomavirus infection in the third trimester in asymptomatic women. J Soc Gynecol
Investig 2005;12:440-444.
40. Hahn HS, Kee MK, Kim HJ, Kim MY, Kang YS, Park JS, et al. Distribution of maternal and infant human Papillomavirus: risk factors associated with vertical transmission. Eur J Obstet Gynecol Reprod Biol 2013;169:202-206.
41. Ambuhl LM, Baandrup U, Dybkaer K, Blaakaer J, Uldbjerg N, Sorensen S. Human Papillomavirus infection as a possible cause of spontaneous abortion and spontaneous preterm delivery. Infect Dis Obstet Gynecol 2016;2016:3086036.
42. Lara-Díaz VJ, Arízaga-Ballesteros V, Garrido-Rodríguez A, Ruiz-de la Garza R, Moreno-Cuevas JE. Incidence of positive DNA from cytomegalovirus, human Parvovirus B19 and hepatitis B virus, at birth. Salud Publica Mex 2012;54:105-106.
43. Alanen A, Kahala K, Vahlberg T, Koskela P, Vainionpaa R. Seroprevalence, incidence of prenatal infections and reliability of maternal history of varicella zoster virus, cytomegalovirus, herpes simplex virus and Parvovirus B19 infection in south-western Finland. BJOG 2005;112:50-56.
44. Behzad-Behbahani A, Pouransari R, Tabei SZ, Rahiminejad MS, Robati M, Yaghobi R, et al. Risk of viral transmission via bone marrow progenitor cells versus umbilical cord blood hematopoietic stem cells in bone marrow transplantation. Transplant Proc 2005;37:3211-3212.
45. Sohrabi A, Samarbafzadeh A, Makvandi M, Maraghi S, Razi T, Darban D. A seroepidemiological study of Parvovirus B19, Toxoplasma gondii and Chlamydia trachomatis in pregnant women referring to Obs & Gyn ward of Ahwaz Imam Khomeini hospital. J Reprod Infertil 2007;8:171-175.
2. Rogers I, Casper RF. Umbilical cord blood stem cells. Best Pract Res Clin Obstet Gynaecol 2004;18:893-908.
3. Eapen M, Rubinstein P, Zhang MJ, Stevens C, Kurtzberg J, Scaradavou A, et al. Outcomes of transplantation of unrelated donor umbilical cord blood and bone marrow in children with acute leukaemia: a comparison study. Lancet 2007;369:1947-1954.
4. Aghideh AN, Kheirandish M, Abolghasemi H, Gharehbaghian A. Platelet growth factors suppress ex vivo expansion and enhance differentiation of umbilical cord blood CD133+ stem cells to megakaryocyte progenitor cells. Growth Factors 2010;28:409-416.
5. Noroozi-Aghideh A, Kheirandish M. Human cord blood-derived viral pathogens as the potential threats to the hematopoietic stem cell transplantation safety: A mini review. World J Stem Cells 2019;11:73-83.
6. Meissner-Roloff M, Gaggia L, Vermeulen M, Mazanderani AFH, du Plessis NM, Steel HC, et al. Strategies for screening cord blood for a public cord blood bank in high HIV prevalence regions. Glob Health Epidemiol Genom 2018;3:e9.
7. de Pagter PJ, Schuurman R, Visscher H, de Vos M, Bierings M, van Loon AM, et al. Human herpes virus 6 plasma DNA positivity after hematopoietic stem cell transplantation in children: an important risk factor for clinical outcome. Biol Blood Marrow Transplant 2008;14:831-839.
8. Abiodun I, Opaleye OO, Ojurongbe O, Fagbami AH. Seroprevalence of parvovirus B19 IgG and IgM antibodies among pregnant women in Oyo State, Nigeria. J Infect Dev Ctries 2013;7:946-950.
9. Ergaz Z, Ornoy A. Parvovirus B19 in pregnancy. Reprod Toxicol 2006;21:421-435.
10. Aberham C, Pendl C, Gross P, Zerlauth G, Gessner M. A quantitative, internally controlled real-time PCR Assay for the detection of Parvovirus B19 DNA. J Virol Methods 2001;92:183-191.
11. Laub R, Strengers P. Parvoviruses and blood products. Pathol Biol (Paris) 2002;50:339-348.
12. Lehmann HW, von Landenberg P, Modrow S. Parvovirus B19 infection and autoimmune disease. Autoimmun Rev 2003;2:218-223.
13. Giorgio E, De Oronzo MA, Iozza I, Di Natale A, Cianci S, Garofalo G, et al. Parvovirus B19 during pregnancy: a review. J Prenat Med 2010;4:63-66.
14. Hokynar K, Norja P, Laitinen H, Palomaki P, Garbarg-Chenon A, Ranki A, et al. Detection and differentiation of human Parvovirus variants by commercial quantitative real-time PCR tests. J Clin Microbiol 2004;42:2013-2019.
15. Lunardi C, Tinazzi E, Bason C, Dolcino M, Corrocher R, Puccetti A. Human Parvovirus B19 infection and autoimmunity. Autoimmun Rev 2008;8:116-120.
16. Riipinen A, Vaisanen E, Nuutila M, Sallmen M, Karikoski R, Lindbohm ML, et al. Parvovirus b19 infection in fetal deaths. Clin Infect Dis 2008;47:1519-1525.
17. Crane J, Mundle W, Boucoiran I, Maternal Fetal Medicine C. Parvovirus B19 infection in pregnancy. J Obstet Gynaecol Can 2014;36:1107-1116.
18. Bonvicini F, Manaresi E, Gallinella G, Gentilomi GA, Musiani M, Zerbini M. Diagnosis of fetal parvovirus B19 infection: value of virological assays in fetal specimens. BJOG 2009;116:813-817.
19. Shahrokhi S, Ebtekar M, Alimoghaddam K, Pourfathollah AA, Kheirandish M, Ardjmand A, et al. Substance P and calcitonin gene-related neuropeptides as novel growth factors for ex vivo expansion of cord blood CD34(+) hematopoietic stem cells. Growth Factors 2010;28:66-73.
20. Golchin N, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z. Quantification of viral genome in cord blood donors by real time PCR to investigate human Herpesvirus type 8 active infection. Transfus Apher Sci 2015;53:378-380.
21. Abedi E, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z, et al. Quantitative polymerase chain reaction for detection of human Herpesvirus-7 infection in umbilical cord blood donors. Transpl Infect Dis 2015;17:21-24.
22. Abedi E, Kheirandish M, Sharifi Z, Samiee S, Kokhaei P, Pourpak Z, et al. Quantification of active and latent form of human Cytomegalovirus infection in umbilical cord blood donors by Real-Time PCR. Int J Organ Transplant Med 2017;8:140-145.
23. Marhamati N, Shirvani F, Azimi L, Hashemieh M, Hosseini A, Asad Shahmirzadi S, et al. Molecular detection of human Parvovirus B19 in umbilical cord blood: case study of Tehran, Iran. Gene Reports 2021;23:101183.
24. Norbeck O, Papadogiannakis N, Petersson K, Hirbod T, Broliden K, Tolfvenstam T. Revised clinical presentation of Parvovirus B19-associated intrauterine fetal death. Clin Infect Dis 2002;35:1032-1038.
25. Landolsi H, Yacoubi MT, Bouslama L, Lahmar A, Trabelsi A, Hmissa S, et al. Detection of the human Parvovirus B19 in nonimmune hydrops fetalis using immunohistochemistry and nested-PCR in formalin-fixed and paraffin-embedded placenta and fetal tissues. Pathol Biol (Paris) 2009;57(3):e1-7.
26. Heegaard ED, Brown KE. Human Parvovirus B19. Clin Microbiol Rev 2002;15:485-505.
27. Slavov SN, Rodrigues ES, Sauvage V, Caro V, Diefenbach CF, Zimmermann AM, et al. Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil. J Med Virol 2019;91:1224-1231.
28. Heegaard ED, Petersen BL, Heilmann CJ, Hornsleth A. Prevalence of Parvovirus B19 and Parvovirus V9 DNA and antibodies in paired bone marrow and serum samples from healthy individuals. J Clin Microbiol 2002;40:933-936.
29. Tolfvenstam T, Broliden K. Parvovirus B19 infection. Semin Fetal Neonatal Med 2009;14:218-221.
30. Mendelson E, Aboudy Y, Smetana Z, Tepperberg M, Grossman Z. Laboratory assessment and diagnosis of congenital viral infections: Rubella, Cytomegalovirus (CMV), Varicella-zoster virus (VZV), Herpes Simplex Virus (HSV), Parvovirus B19 and human immunodeficiency virus (HIV). Reprod Toxicol 2006;21:350-382.
31. Zhou Y, Bian G, Zhou Q, Gao Z, Liao P, Liu Y, et al. Detection of cytomegalovirus, human Parvovirus B19, and herpes simplex viru“ 1/2 in women with firs” trimester spontaneous abortions. J Med Virol 2015;87:1749-1753.
32. Tognon M, Tagliapietra A, Magagnoli F, Mazziotta C, Oton-Gonzalez L, Lanzillotti C, et al. Investigation on spontaneous abortion and human Papillomavirus infection. Vaccines (Basel) 2020;8:473.
33. Parsania M, Bamdad T, Hassan ZM, Kheirandish M, Pouriayevali MH, Sari RD, et al. Evaluation of apoptotic and anti-apoptotic genes on efficacy of DNA vaccine encoding glycoprotein B of Herpes Simplex virus type 1. Immunol Lett 2010;128:137-142.
34. Meshkat Z, Soleimanjahi H, Mahmoudi M, Hassan ZM, Mirshahabi H, Meshkat M, et al. CTL responses to a DNA vaccine encoding E7 gene of human Papillomavirus type 16 from an Iranian isolate. Iran J
Immunol 2008;5:82-91.
35. Servey JT, Reamy BV, Hodge J. Clinical presentations of Parvovirus B19 infection. Am Fam Physician 2007;75:373-376.
36. Rollin R, Alvarez-Lafuente R, Marco F, Jover JA, Hernandez-Garcia C, Rodriguez-Navas C, et al. Human Parvovirus B19, Varicella zoster virus, and human Herpesvirus-6 in mesenchymal stem cells of patients with osteoarthritis: analysis with quantitative real-time polymerase chain reaction. Osteoarthritis Cartilage 2007;15:475-478.
37. Teixeira LO, Amaral SC, Finger-Jardim F, da Hora VP, Goncalves CV, Soares MA, et al. [Frequency of human Papillomavirus in the placenta, in the colostrum and in the umbilical cord blood]. Rev Bras Ginecol Obstet 2015;37:203-207.
38. Koskimaa HM, Waterboer T, Pawlita M, Grenman S, Syrjanen K, Syrjanen S. Human Papillomavirus genotypes present in the oral mucosa of newborns and their concordance with maternal cervical human papillomavirus genotypes. J Pediatr 2012;160:837-843.
39. Worda C, Huber A, Hudelist G, Schatten C, Leipold H, Czerwenka K, et al. Prevalence of cervical and intrauterine human Papillomavirus infection in the third trimester in asymptomatic women. J Soc Gynecol
Investig 2005;12:440-444.
40. Hahn HS, Kee MK, Kim HJ, Kim MY, Kang YS, Park JS, et al. Distribution of maternal and infant human Papillomavirus: risk factors associated with vertical transmission. Eur J Obstet Gynecol Reprod Biol 2013;169:202-206.
41. Ambuhl LM, Baandrup U, Dybkaer K, Blaakaer J, Uldbjerg N, Sorensen S. Human Papillomavirus infection as a possible cause of spontaneous abortion and spontaneous preterm delivery. Infect Dis Obstet Gynecol 2016;2016:3086036.
42. Lara-Díaz VJ, Arízaga-Ballesteros V, Garrido-Rodríguez A, Ruiz-de la Garza R, Moreno-Cuevas JE. Incidence of positive DNA from cytomegalovirus, human Parvovirus B19 and hepatitis B virus, at birth. Salud Publica Mex 2012;54:105-106.
43. Alanen A, Kahala K, Vahlberg T, Koskela P, Vainionpaa R. Seroprevalence, incidence of prenatal infections and reliability of maternal history of varicella zoster virus, cytomegalovirus, herpes simplex virus and Parvovirus B19 infection in south-western Finland. BJOG 2005;112:50-56.
44. Behzad-Behbahani A, Pouransari R, Tabei SZ, Rahiminejad MS, Robati M, Yaghobi R, et al. Risk of viral transmission via bone marrow progenitor cells versus umbilical cord blood hematopoietic stem cells in bone marrow transplantation. Transplant Proc 2005;37:3211-3212.
45. Sohrabi A, Samarbafzadeh A, Makvandi M, Maraghi S, Razi T, Darban D. A seroepidemiological study of Parvovirus B19, Toxoplasma gondii and Chlamydia trachomatis in pregnant women referring to Obs & Gyn ward of Ahwaz Imam Khomeini hospital. J Reprod Infertil 2007;8:171-175.
| Files | ||
| Issue | Vol 14 No 1 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v14i1.8813 | |
| Keywords | ||
| Parvoviridae; Human parvovirus B19; Umbilical cord blood; Hematopoietic stem cells; Nested polymerase chain reaction | ||
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How to Cite
1.
Ramezany H, Kheirandish M, Samiee S, Khosravifar M, Hashemi SM. Investigation of human parvovirus B19 prevalence in a large healthy umbilical cord blood donors. Iran J Microbiol. 2022;14(1):119-124.
