Original Article

Effect of giving probiotic supplement Lactobacillus acidophilus La-14 as an immunomodulator to maintain a respiratory system in Mus musculus

Abstract

Background and Objectives: In 2020 the whole world is experiencing a pandemic condition due to infection with the SARS-CoV-2 virus which can cause the COVID-19 disease. This condition results in “Panic Buying”, because everyone tries to avoid the spread and transmission of the COVID-19 disease by doing various ways, one of which is by taking additional supplements such as vitamin C and probiotic supplements.
Materials and Methods: The materials used were mice Mus musculus male DDY strain aged 1-2 months. Probiotic supplement Lactobacillus acidophilus La-14 with a viability of 1 × 108 CFU/ml. with a weight of 0.16 grams dissolved in 0.25 ml 0.9% NaCl. Vitamin C used is a commercial vitamin C tablet, weighing 0.06 grams in 0.25 ml 0.9% NaCl. Meanwhile, the feed for mice (Mus musculus) is a complete feed from Pokphand with the code BR1 CP511B. Lung histology preparations data were analysed descriptively and statistically through the test Chi square while the data on the number of lymphocytes were analysed descriptively.
Results: The histological observations of lungs of Mus musculus showed that in the treatment of ML, MV, and MKA test was carried out chi square ratio between the groups that did not have lymphocyte infiltration and those that had lymphocyte infiltration showed a significant difference (p <0.05). Meanwhile, the results of the lymphocyte count showed that ML and MV treatment was higher than that of MK treatment.
Conclusion: It is suggested that the administration of probiotics can stimulate and modulate the respiratory immune system.

1. Arafat SMY, Kar SK, Menon V, Kaliamoorthy C, Mukherjee S, Alradie-Mohamed A, et al. Panic buying: an insight from the content analysis of media reports during COVID-19 pandemic. Neurol Psychiatry Brain Res 2020;37:100-103.
2. Ruiz ML, Owatari MS, Yamashita MM, Ferrarezi JVS, Garcia P, Cardoso L, et al. Histological effects on the kidney, spleen, and liver of Nile tilapia Oreochromis niloticus fed different concentrations of probiotic Lactobacillus plantarum. Trop Anim Health Prod 2020;52:167-176.
3. Singh KS, Kumar S, Mohanty AK, Grover S, Kaushik JK. Mechanistic insights into the host-microbe interaction and pathogen exclusion mediated by the mucus-binding protein of Lactobacillus plantarum. Sci Rep 2018;8:14198.
4. Abbasia A, Aghebati-Maleki A, Yousefi M, Aghebati-Maleki L. Probiotic intervention as a potential therapeutic for managing gestational disorders and improving pregnancy outcomes. J Reprod Immunol 2021;143:103244.
5. Rusli, Amalia F, Dwyana Z. Potential of Lactobacillus Acidophilus bacteria as antidiare and immunomodulator. J Biol Makassar 2018;3:25-30.
6. Foysal MJ, Fotedar R, Siddik MAB, Tay A. Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii). Sci Rep 2020;10:5916.
7. Mashayekhi H, Mazhari M, Esmaeilipour O. Eucalyptus leaves powder, antibiotic and probiotic addition to broiler diets: effect on growth performance, immune response, blood components and carcass traits. Animal 2018;12:2049-2055.
8. Back SJ, Park SJ, Moon JS, Lee SB, Jo SJ, Nam TJ, et al. The effects of dietary heat-killed probiotics bacteria additives in low-fishmeal feed on growth performance, immune responses, and intestinal morphology in juvenile olive flounder paralichthys olivaceus. Aquac Reports 2020;18:100415.
9. Mnif S, Elkosantini S, Darmoul S, Ben Said L. An immune memory and negative selection based decision support system to monitor and control public bus transportation Systems. IFAC-PapersOnLine 2016;49:143-148.
10. Lee SJ, Chinen J, Kavanaugh A. Immunomodulator therapy: monoclonal antibodies, fusion proteins, cytokines, and immunoglobulins. J Allergy Clin Immunol 2010;125(2 Suppl 2):S314-323.
11. Chan LS. Ocular and oral mucous membrane pemphigoid (cicatricial pemphigoid). Clin Dermatol 2012;30:34-37.
12. Hussey Freeland DM, Fan-Minogue H, Spergel JM, Chatila TA, Nadeau KC. Advances in food allergy oral immunotherapy: toward tolerance. Curr Opin Immunol 2016;42:119-123.
13. Sekkat A, Bouchal S, Charifi Y, Hajjam KE, Alaoui YL, Belahsen MF, et al. Pulmonary and intracranial miliary tuberculosis secondary to Behçet’s anti-TNF alfa treatment. Radiol Case Rep 2020;16:338-342.
14. Suva LJ, Cooper A, Watts AE, Ebetino FH, Price J, Gaddy D. Bisphosphonates in veterinary medicine: the new horizon for use. Bone 2021;142:115711.
15. Lau I, Potluri A, Ibeh CL, Redman RS, Paal E, Bandyopadhyay BC. Microcalcifications in stone-obstructed human submandibular gland are associated with apoptosis and cell proliferation. Arch Oral Biol 2017;82:99-108.
16. Cheng T, Feng Y, Chen X, Zhou J, Song Y. Lung-resident mesenchymal stem cells regulated the inflammatory responses in innate and adaptive immune cells through HVEM-BTLA pathway during ARDS. Exp Cell Res 2020;395:112155.
17. Lyu M, Liu H, Ye Y, Yin Z. Inhibition effect of thiol-type antioxidants on protein oxidative aggregation caused by free radicals. Biophys Chem 2020;260:106367.
18. Sannasimuthu A, Arockiaraj J. Intracellular free radical scavenging activity and protective role of mammalian cells by antioxidant peptide from thioredoxin disulfide reductase of Arthrospira platensis. J Funct Foods 2019;61:103513.
19. Shakoor H, Feehan J, Al Dhaheri AS, Ali HI, Platat C, Ismail LC, et al. Immune-boosting role of vitamins D, C, E, zinc, selenium and omega-3 fatty acids: could they help against COVID-19? Maturitas 2021;143:1-9.
20. Alawneh JI, Barreto MO, Moore RJ, Soust M, Al-harbi H, James AS, et al. Systematic review of an intervention: the use of probiotics to improve health and productivity of calves. Prev Vet Med 2020;183:105147.
21. Wahyuningsih R, Darmono SS, Margawati A. Effect of probiotics Lactobacillus helveticus Rosell-52 and Lactobacillus rhamnosus Rosell-11 on the lymphocyte levels of the elderly. Indones Nutr J 2014;3:13-19.
22. Dushku E, Ioannou A, Staikou A, Yiangou M. Probiotic properties and immunomodulatory activity of gastrointestinal tract commensal bacterial strains isolated from the edible farmed snail Cornu aspersum maxima. Fish Shellfish Immunol 2019;92:792-801.
23. Ma T, Suzuki Y, Guan LL. Dissect the mode of action of probiotics in affecting host-microbial interactions and immunity in food producing animals. Vet Immunol Immunopathol 2018;205:35-48.
24. Place DE, Kanneganti TD. The innate immune system and cell death in autoinflammatory and autoimmune disease. Curr Opin Immunol 2020;67:95-105.
25. Li T, Teng D, Mao R, Hao Y, Wang X, Wang J. A critical review of antibiotic resistance in probiotic bacteria. Food Res Int 2020;136:109571.
26. Sun L, Wang X, Saredy J, Yuan Z, Yang X, Wang H. Innate-adaptive immunity interplay and redox regulation in immune response. Redox Biol 2020;37:101759.
27. Qin X, Liu J, Du Y, Li Y, Zheng L, Chen G, et al. Different doses of vitamin C supplementation enhances the Th1 immune response to early Plasmodium yoelii 17XL infection in BALB/c mice. Int Immunopharmacol 2019;70:387-395.
28. Szczypka M, Sobieszczańska A, Suszko-Pawłowska A, Lis M. Selegiline and clomipramine effects on lymphocyte subsets, regulatory T cells and sheep red blood cell (SRBC)-induced humoral immune response after in vivo administration in mice. Eur J Pharmacol 2020; 887:173560.
29. De la Fuente M, Sánchez C, Vallejo C, Díaz-Del Cerro E, Arnalich F, Hernanz Á. Vitamin C and vitamin C plus E improve the immune function in the elderly. Exp Gerontol 2020;142:111118.
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IssueVol 13 No 3 (2021) QRcode
SectionOriginal Article(s)
Published2021-06-05
Keywords
Lactobacillus acidophilus; Vitamin C; Lymphocyte infiltration; Lymphocytes; Mus musculus

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1.
Artanti D, Sispita Sari YE, Azizah F, Puwaningsih NV, Rohmayani V, Nasrullah D. Effect of giving probiotic supplement Lactobacillus acidophilus La-14 as an immunomodulator to maintain a respiratory system in Mus musculus. Iran J Microbiol. 13(3):381-388.