Antimicrobial activities of microbial essential fatty acid against foodborne pathogenic bacteria
Background and Objectives: Human health and development have been related to dietary intake of essential fatty acids (omega 3, 6 and 9) and important for brain development, immune system function, and blood pressure regulation. Microbial essential oils are more natural and safer alternatives to synthetic preservatives. These oils have been demonstrated to have antibacterial activity within food systems and may be ideal additives to food formulations. Zygomycete fungi are well-known as good candidate for production of essential oils.
Materials and Methods: Essential oils of fungi Mucor rouxii, Mucor circinelloides and Cuninghamella echinulata were extracted and fatty acids were analyzed by GC, for the first, antimicrobial activity of the fungi essential oils against foodborne pathogenic bacteria E. coli, S. aureus, B. cereus, B. subtilis, and S. enterica was examined by disc diffusion and well diffusion methods and the minimal inhibitory concentrations (MIC) of oils were determined by microtiter plate.
Results: The fungi oils were exhibited the stron g antibacterial effect against Gram-positive bacteria, B. cereus, S. aureus and B. subtilis higher than Gram-negative and commercial oleic acid and linoleic acid. The MIC of the fungi oil extracts was 0.25 mg/ml for B. cereus and B. subtilis and 0.5 mg/ml about S. aureus. This research demonstrated microbial essential oils may be suitable for their antimicrobial properties in food.
Conclusion: Microbial essential oil with good antibacterial activity could also be used in selected cases like foodborne disease.
2. Sprague M, Desbois AP. Fatty acid and lipid class composition in cutaneous mucus of Atlantic salmon. Aquac Res 2021; 52: 6808-6813.
3. Sokoła-Wysoczańska E, Wysoczański T, Wagner J, Czyż K, Bodkowski R, Lochyński S, et al. Polyunsaturated fatty acids and their potential therapeutic role in cardiovascular system disorders-a review. Nutrients 2018; 10: 1561.
4. Mirbagheri M, Nahvi I, Emamzadeh R. Reduction of chemical and biological oxygen demands from oil wastes via oleaginous fungi: An attempt to convert food by products to essential fatty acid. Iran J Biotechnol 2015; 13: 25-30.
5. Li ZH, Cai M, Liu YS, Sun PL, Luo SL. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules 2019; 24: 1577.
6. Glick NR, Fischer MH. The role of essential fatty acids in human health. J Evid Based Complementary Altern Med 2013; 18: 268-289.
7. Kim YG, Lee JH, Raorane CJ, Oh ST, Park JG, Lee J. Herring oil and omega fatty acids inhibit Staphylococcus aureus biofilm formation and virulence. Front Microbiol 2018; 9: 1241.
8. Jung SW, Lee SW. The antibacterial effect of fatty acids on Helicobacter pylori infection. Korean J Intern Med 2016; 31: 30-35.
9. Bonilla DL, Ly LH, Fan YY, Chapkin RS, McMurray DN. Incorporation of a dietary omega 3 fatty acid impairs murine macrophage responses to Mycobacterium tuberculosis. PLoS One 2010; 5(5): e10878.
10. Patil SD, Sharma R, Srivastava S, Navani NK, Pathania R. Downregulation of yidC in Escherichia coli by antisense RNA expression results in sensitization to antibacterial essential oils eugenol and carvacrol. PLoS One 2013; 8(3): e57370.
11. Papanikolaou S, Galiotou-Panayotou M, Fakas S, Komaitis M, Aggelis G. Lipid production by oleaginous mucorales cultivated on renewable carbon sources. Eur J Lipid Sci Technol 2007; 109: 1016-1070.
12. Naviglio D, DellaGreca M, Ruffo F, Andolfi A, Gallo, M. Rapid analysis procedures for triglycerides and fatty acids as pentyl and phenethyl esters for the detection of butter adulteration using chromatographic techniques. J Food Qual 2017; 2017: 1-11.
13. Mirbagheri M, Nahvi I, Emamzadeh R, Emtiazi G, Shirani E. Oil wastes management: medium optimization for the productionof alpha-linolenic acid in Mucor circinelloides. Int J Environ Sci Technol 2016; 13: 31-38.
14. Akhbariyoon H, Azizpour Y, Fakhrizadeh Esfahani M, Firoozabad MSM, Rad MR, Esfahani KS, et al. Immune checkpoint inhibition for the treatment of cancers: An update and critical review of ongoing clinical trials. Clin Immunol 2021; 232: 108873.
15. Zheng CJ, Yooa JS, Lee TG, Cho HY, Kim YH, Kim WG. Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett 2005; 579: 5157-5162.
16. Fischera CL, Drakea DR, Dawson DV, Blanchette DR, Brogden KA, Wertz PW. Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria. Antimicrob Agents Chemother 2012; 56: 1157-1161.
17. Mamatha SS, Ravi R, Venkateswaran G. Medium optimization of gamma linolenic acid production in Mucor rouxii CFR -G15 using RSM. Food Bioproc Tech 2008; 1: 405-409.
18. Chanda W, Joseph TP, Guo XF, Wang WD, Liu M, Vuai MS, et al. Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens. J Zhejiang Univ Sci B 2018; 19: 253-262.
19. Yoon BK, Jackman JA, Valle-González ER, Cho NJ. Antibacterial free fatty acids and monoglycerides: biological activities, experimental testing, and therapeutic applications. Int J Mol Sci 2018; 19: 1114.
|Issue||Vol 14 No 2 (2022)|
|Anti-bacterial agent; Fatty acid essential; Omega 6; Microbial sensitivity tests; Foodborne diseases|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|