Original Article

Investigating the effect of pH, different concentrations of glutamate acid and salt on production in low-fat probiotic cheese

Abstract

Background and Objectives: Gamma-aminobutyric acid (GABA) is a non-protein amino acid produced by lactic acid bacteria. Among GABA-producing bacteria, lactic acid bacteria have received more attention due to their probiotic nature and properties such as inhibiting pathogenic bacteria, strengthening the immune system, and so on.
Materials and Methods: Investigation on the effect of three independent variables including pH (4.7, 4.9 and 5.1), glutamic acid (1, 2 and 3 mgg-1) and salt (2, 2.5 and 3%) on GABA production in low fat cheese by probiotic bacteria.
Results: By increasing the amount of glutamic acid and decreasing the pH from 5.1 to 4.7, the amount of GABA production in ultra-filtration cheese significantly increased on the 15th and 30th days of production (p≤0.05), while by increasing the amount of salt the production GABA decreased on the 15th and 30th days. Simultaneous optimal conditions to achieve maximum GABA production in cheese on the 15th and 30th production day was respectively 167.7917 mg/kg-1 and 220.125 mg/kg-1 using 3 mg/g glutamic acid, 2% salt at pH 4.7.
Conclusion: The results showed that by identifying probiotic bacteria with the highest potential for GABA production and optimizing the culture medium, more GABA can be produced in food products and a positive step can be taken to produce pragmatic products and promote consumer health.

1. Fox P, McSweeney P, Cogan T, Guinee T (2004). Cheese: chemistry, physics, and microbiology. 3rd ed. Elsevier, Amsterdam.
2. Coman MM, Cecchini C, Verdenelli MC, Silvi S, Orpianesi C, Cresci A. Functional foods as carriers for SYNBIO®, a probiotic bacteria combination. Int J Food Microbiol 2012;157:346-352.
3. Topping DL, Clifton PM. Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 2001;8:1031-1064.
4. Zhao A, Hu X, Pan L, Wang X. Isolation and characterization of a gamma-aminobutyric acid producing strain Lactobacillus buchneri WPZ001 that could efficiently utilize xylose and corncob hydrolysate. Appl Microbiol Biotechnol 2015;99:3191-3200.
5. Kheiri-Yegane Azar B, Esmaeili A, Rabbani M. Cloning of glutamate decarboxylase and production of γ- aminobutyric acid from Lactobacillus brevis. Res Pharm Sci 2012;7:12-20.
6. Mazzoli R, Pessione E, Dufour M, Laroute V, Giuffrida MG, Giunta C, et al. Glutamate-induced metabolic changes in Lactococcus lactis NCDO 2118 during GABA production: combined transcriptomic and proteomic analysis. Amino Acids 2010;39:727-737.
7. Lee EJ, Lee SP. Novel bioconversion of sodium glutamate to γ-Amino butyric acid by co-culture of Lactobacillus plantarum K154 in Ceriporia lacerata culture broth. Food Sci Biotechnol 2014;23:1997-2005.
8. Li H, Qiu T, Huang G, Cao Y. Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentation. Microb Cell Fact 2010;9:85.
9. Komatsuzaki N, Shima J, Kawamotoa S, Momosed H, Kimurab T. Production of γ-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 2005;22:497-504.
10. Siragusa S, De Angelis M, Di Cagno R, Rizzello CG, Coda R, Gobbetti M. Synthesis of gamma-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl Environ Microbiol 2007;73:7283-7290.
11. Lacroix N, St-Gelais D, Champagne CP, Vuillemard JC. Gamma-aminobutyric acid-producing abilities of lactococcal strains isolated from old-style cheese starters. Dairy Sci Technol 2013;93:315-327.
12. Wu CH, Hsueh YH, Kuo JM, Liu SJ. Characterization of a potential probiotic Lactobacillus brevis RK03 and efficient production of γ-aminobutyric acid in batch fermentation. Int J Mol Sci 2018;19:143.
13. Sharafi S, Nateghi L. Optimization of gamma-aminobutyric acid production by probiotic bacteria through response surface methodology. Iran J Microbiol 2020;12:584-591.
14. Sharafi S, Nateghi L, Eyvazzade O, Ebrahimi MTA. The physicochemical, texture hardness and sensorial properties of ultrafiltrated low-fat cheese containing galactomannan and novagel gum. Acta Sci Pol Technol Aliment 2020;19:83-100.
15. Bartolomeo MP, Maisano, F. Validation of a reversed-phase HPLC method for quantitative amino acid analysis. J Biomol Tech 2006;17:131-137.
16. Dhakal R, Bajpai VK, Baek KH. Production of GABA (gamma-aminobutyric acid) by microorganisms: a review. Braz J Microbiol 2012;43:1230-1241.
17. Cho YR, Chang JY, Chang HC. Production of gamma-aminobutyric acid (GABA) by Lactobacillus buchneri isolated from kimchi and its neuroprotective effect on neuronal cells. J Microbiol Biotechnol 2007;17:104-109.
18. Lu X, Chen Z, Gu Z, Han Y. Isolation of γ-aminobutyric acid-producing bacteria and optimization of fermentative medium. Biochem Eng J 2008;41:48-52.
19. Yang SY, Lu FX, Lu ZX, Bie XM, Jiao Y, Sun LJ, et al. Production of gamma-aminobutyric acid by Streptococcus salivarius subsp. thermophilus Y2 under submerged fermentation. Amino Acids 2008;34:473-478.
20. Di Cagno R, Mazzacane F, Rizzello CG, De Angelis M, Giuliani G, Meloni M, et al. Synthesis of γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications. Appl Microbiol Biotechnol 2010;86:731-741.
21. Iimure TM, Kihara N, Hirota T, Zhou k, Hayashi K, Ito A. Method for production of gamma-amino butyric acid (GABA) using barley bran supplemented with glutamate. Food Res Int 2009;42:319-323.
22. Hiraga K, Ueno Y, Oda K. Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate. Biosci Biotechnol Biochem 2008;72:1299-1306.
23. Park KB, Oh SH. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresour Technol 2007; 98:1675-1679.
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IssueVol 13 No 3 (2021) QRcode
SectionOriginal Article(s)
Published2021-06-05
Keywords
Gamma-aminobutyric acid; Low fat cheese; Probiotic bacteria

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How to Cite
1.
Sharafi S, Nateghi L, Yousefi S. Investigating the effect of pH, different concentrations of glutamate acid and salt on production in low-fat probiotic cheese. Iran J Microbiol. 13(3):389-398.