Original Article

Antimicrobial activity of bioactive compounds of Haplopappus multifolius and Haplopappus taeda against human pathogenic microorganisms


Background and Objectives: Haplopappus multifolius Phil. Ex Reiche and Haplopappus taeda Reiche are medicinal shrubs native to Chile and are popularly known as "Bailahuén". Regularly, this plant is used for liver, digestive and renal affections, as well as colds and the cleaning of infected wounds. The aim of the study was to identify the responsible compounds for the antimicrobial activity of H. multifolius and H. taeda.
Materials and Methods: Infusions and ethanolic extracts of H. taeda and H. multifolius were analysed by thin-layer chromatography bioautography (TLC-B) to determine the compounds responsible for the antimicrobial activity against Gram-positive and Gram-negative bacterial strains and yeasts of Bailahuén. Finally, the minimum inhibitory concentration (MIC) of pure compounds isolated was determinate.
Results: Extract of Bailahuén had activity only against Gram-positive bacterial strains and this activity was associated with aesculetin, 18-acetoxy-cis-cleroda-3,13E-dien-15-oic acid and aromadendrin-7-methyl ether compounds.
Conclusion: H. multifolius and H. taeda have antibacterial capacity on different species of Gram-positive bacteria pathogenic for humans.

1. Gardam MA. Is methicillin-resistant Staphylococcus aureus an emerging community pathogen? A review of the literature. Can J Infect Dis 2000; 11: 202-211.
2. Alviano DS, Alviano CS. Plant extracts: search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol 2009; 10: 106-121.
3. WHO (2002). Traditional Medicine Growing Needs and Potential - WHO Policy Perspectives on Medicines, No. 002, May, World Health Organization, Geneva, Switzerland.
4. Vogel H, González M, Faini F, Razmilic I, Rodríguez J, San Martín J, et al. Antioxidant propierties and TLC characterization of tour Chilean Haplopappus- species known as bailahuén. J Ethnopharmacol 2005; 97: 97-100.
5. Muñoz M, Barrera E, and Meza I (1981). El uso medicinal y alimenticio de plantas nativas y naturalizadas en Chile. Publicación Ocasional, 33, Museo Nacional de Historia Natural, Santiago.
6. Urzúa A, Torres R, Muñoz M, Palacios Y. Comparative antimicrobial study of the resinous exudates of some Chilean Haplopappus (Asteraceae). J Ethnopharmacol 1995; 45: 71-74.
7. Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sokmen A, et al. Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). J Ethnopharmacol 2003; 87: 215-220.
8. Murillo-Alvarez J, Franzblau S. Antimicrobial and cytotoxic activity of some medicinal plants from Baja California Sur (México). Pharm. Biol 2001; 39: 445-449.
9. Urzúa A, Torres R, Mendoza I, Delle Monache F. Antibacterial new clerodane diterpenes from the surface of Haplopappus foliosus. Planta Med 2003; 69: 675-677.
10. Chiang MT, Bittner M, Silva M, Mondaca A, Zemelman R, Sammes PG. A prenylated coumarin with antimicrobial activity from Haplopappus multifolius. Phytochemistry 1982; 21: 2753-2755.
11. Torres R, Faini F, Modak B, Urbina F, Labbe C, Guerrero J. Antioxidant activity of coumarins and flavonols from the resinous exudate of Haplopappus multifolius. Phytochemistry 2006; 67: 984-987.
12. Faini F, Labbé C, Torres R, Rodilla J, Silva L, Delle Monache F. New phenolic esters from the resinous exudate of Haplopappus taeda. Fitoterapia 2007; 78: 611-613.
13. CLSI (2015). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI Document M100-S25. http://www.clsi.org/
14. Biswanath D, Reddy MR, Ramu R, Ravindranath N, Harish H, Ramakrishna KV, et al. Clerodane diterpenoids from Pulicaria wightiana. Phytochemistry 2005; 66:633-638.
15. Murthy MM, Subramanyam M, Bindu Hima M, Annapurna J. Antimicrobial activity of clerodane diterpenoids from Polyathia longifolia seeds. Fitoterapia 2005; 76: 336-339.
16. Urzúa A, Jara F, Tojo E, Wilkens M, Mendoza L, Rezende MC. A new antibacterial clerodane diterpenoid from the resinous exudates of Haplopappus uncinatus. J Ethnopharmacol 2006; 103: 297-301.
IssueVol 13 No 1 (2021) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijm.v13i1.5498
Haplopappus taeda; Haplopappus multifolius; Flavonoids; Terpenoids; Sesculetin

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How to Cite
Padilla C, Lobos O, Poblete-Tapia P, Carrasco-Sánchez V. Antimicrobial activity of bioactive compounds of Haplopappus multifolius and Haplopappus taeda against human pathogenic microorganisms. Iran J Microbiol. 13(1):98-103.