Antibacterial properties of selected Malaysian Tualang honey against Pseudomonas aeruginosa and Streptococcus pyogenes

  • Mohammad Abdulraheem Al-Kafaween Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
  • Hamid Ali Nagi Al-Jamal Mail Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
  • Abu Bakar Mohd Hilmi Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
  • Nour Amin Elsahoryi Department of Nutrition, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan
  • Norzawani Jaffar Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
  • Mohd Khairi Zahri Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia
Keywords:
Antibacterial activity;, Tulang honey;, Scanning electron microscopy;, Differential expression

Abstract

Background and Objectives: Tualang honey (TH) is a Malaysian multifloral jungle honey. In recent years, there has been a marked increase in the number of studies published in medical databases regarding its potential health benefits. The study aimed to investigate the effect of TH against Pseudomonas aeruginosa and Streptococcus pyogenes.
Materials and Methods: The effect of TH on both bacteria was investigated using MIC, MBC, growth curve, time-kill curve, scanning electron microscopy (SEM) and RT-qPCR.
Results: The MIC of TH against P. aeruginosa and S. pyogenes was 18.5% (w/v) and 13% (w/v) respectively and MBC was 25% (w/v) for both bacteria. Spectrophotometric readings of at least 90% inhibition yielded MIC90 values of TH, 18.5% (w/v) and 15% (w/v) for P. aeruginosa and S. pyogenes respectively. A time–kill curve demonstrated a bactericidal with a 4-log reduction estimated within 8 hours. Using SEM, loss of structural integrity and marked changes in cell shape were observed. RT-qPCR analysis showed that TH reduced the pattern of gene expression in both bacteria, with a trend toward reduced expression of the virulence genes of interest.
Conclusion: This study suggests that TH could potentially be used as an alternative therapeutic agent for microbial infection particularly against these two organisms.

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Published
2020-12-15
How to Cite
1.
Al-Kafaween M, Al-Jamal H, Mohd Hilmi AB, Elsahoryi N, Jaffar N, Zahri M. Antibacterial properties of selected Malaysian Tualang honey against Pseudomonas aeruginosa and Streptococcus pyogenes. Iran J Microbiol. 12(6):565-576.
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