Surveying the chemical composition and antibacterial activity of essential oils from selected medicinal plants against human pathogens
Abstract
Background and Objectives: Essential oils (EOs) with different biological activities, such as antibacterial properties, are a valuable resource for developing new drugs.
Materials and Methods: Ingredients of six medicinally important EOs, including Artemisia dracunculus, Anethum graveolens, Citrus limon, Citrus sinensis, Cinnamomum zeylanicum and Zingiber officinale, were identified using GC-MS analysis. Moreover, their five major compounds were also listed. Furthermore, the half-maximal inhibitory concentration (IC50) against four important human bacteria was also investigated using the 96-well plate microdilution.
Results: C. sinensis EO with IC50 of 1.0 and 4.7 mg.mL-1 have the most effect on the growth of S. aureus and P. aeruginosa. Moreover, EOs of Cinnamomum zeylanicum (IC50: 1.0 mg. mL-1) and Artemisia dracunculus (IC50: 1.3 mg.mL-1) significantly showed better inhibitory effect on E. coli and K. pneumoniae.
Conclusion: These EOs could be used for developing inexpensive, potent, and green antibacterial agents.
1. Pandey AK, Kumar P, Singh P, Tripathi NN, Bajpai VK. Essential oils: sources of antimicrobials and food preservatives. Front Microbiol 2017;7:2161.
2. Firmino DF, Cavalcante TTA, Gomes GA, Firmino NCS, Rosa LD, de Carvalho MG, et al. Antibacterial and antibiofilm activities of Cinnamomum sp. essential oil and Cinnamaldehyde: antimicrobial activities. ScientificWorldJournal 2018;2018:7405736.
3. Tongnuanchan P, Benjakul S. Essential oils: extraction, bioactivities, and their uses for food preservation. J Food Sci 2014;79:R1231-1249.
4. Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, Jorge AO. Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. J Appl Oral Sci 2013;21:25-31.
5. Nguefack J, Budde BB, Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol 2004;39:395-400.
6. Osanloo M, Sedaghat MM, Esmaeili F, Amani A. Larvicidal activity of essential oil of Syzygium aromaticum (Clove) in com-parison with its major constituent, eugenol, against Anopheles stephensi. J Arthropod Borne Dis 2018;12:361-369.
7. Husain I, Ahmad R, Chandra A, Raza ST, Shukla Y, Mahdi F. Phytochemical characterization and biological activity evaluation of ethanolic extract of Cinnamomum zeylanicum. J Ethnopharmacol 2018;219:110-116.
8. Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 2008;69: 1732-1738.
9. Brochot A, Guilbot A, Haddioui L, Roques C. Antibacterial, antifungal, and antiviral effects of three essential oil blends. Microbiologyopen 2017;6(4):e00459.
10. Man A, Santacroce L, Jacob R, Mare A, Man L. Antimicrobial activity of six essential oils against a group of human pathogens: A comparative study. Pathogens 2019;8:15.
11. Saeb S, Amin M, Gooybari RS, Aghel N. Evaluation of antibacterial activities of Citrus limon, Citrus reticulata, and Citrus grandis against pathogenic bacteria. Int J Enteric Pathog 2016;4(4): e37103.
12. Lambert R, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 2001;91:453-462.
13. Chaleshtori RS, Rokni N, Razavilar V, Kopaei MR. The evaluation of the antibacterial and antioxidant activity of Tarragon (Artemisia dracunculus L.) essential oil and its chemical composition. Jundishapur J Microbiol 2013;6 (9); e7877.
14. Said-Al Ahl HA, Sarhan AM, Dahab ADMA, Abou-Zeid E-SN, Ali MS, Naguib NY, et al. Essential oils of Anethum graveolens L.: chemical composition and their antimicrobial activities at vegetative, flowering and fruiting stages of development. Int J Plant Sci 2015;1:98-102.
15. Kuglerova M, Tesarova H, Grade JT, Halamova K, Wanyana-Maganyi O, Van Damme P, et al. Antimicrobial and antioxidative effects of Ugandan medicinal barks. Afr J Biotechnol 2011;10:3628-3632.
16. Osanloo M, Abdollahi A, Valizadeh A, Abedinpour N. Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms. Iran J Microbiol 2020;12:43-51.
17. Osanloo M, Sedaghat MM, Sereshti H, Rahmani M, Saeedi Landi F, Amani A. Chitosan nanocapsules of tarragon essential oil with low cytotoxicity and long-lasting activity as a green nano-larvicide. J Nanostruct 2019;9:723-735.
18. Valizadeh A, Shirzad M, Esmaeili F, Amani A.Increased antibacterial activity of Cinnamon Oil Microemulsionin Comparison with Cinnamon Oil Bulk and Nanoemulsion. Nanomed Res J 2018;3:37-43.
19. Raeisi M, Tajik H, Razavi RS, Maham M, Moradi M, Hajimohammadi B, et al. Essential oil of tarragon (Artemisia dracunculus) antibacterial activity on Staphylococcus aureus and Escherichia coli in culture media and Iranian white cheese. Iran J Microbiol 2012;4:30-34.
20. Derakhshan S, Navidinia M, Ahmadi A. Antibacterial activity of Dill (Anethum graveolens) essential oil and antibiofilm activity of Cumin (Cuminum cyminum) alcoholic extract. Infect Epidemiol Microbiol 2017;3:122-126.
21. Ruangamnart A, Buranaphalin S, Temsiririrkkul R, Chuakul W, Pratuangdejkul J. Chemical compositions and antibacterial activity of essential oil from dill fruits (Anethum graveolens L.) cultivated in Thailand. Mahidol Univ J Pharm Sci 2015;42:135-143.
22. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 2006;6:39.
23. Eldahshan OA, Halim AF. Comparison of the composition and antimicrobial activities of the essential oils of green branches and leaves of Egyptian navel orange (Citrus sinensis (L.) Osbeck var. Malesy). Chem Biodivers 2016;13:681-685.
24. Frassinetti S, Caltavuturo L, Cini M, Della Croce C, Maserti B. Antibacterial and antioxidant activity of essential oils from Citrus spp. J Essent Oil Res 2011;23:27-31.
25. Debbarma J, Kishore P, Nayak BB, Kannuchamy N, Gudipati V. Antibacterial activity of ginger, eucalyptus and sweet orange peel essential oils on fish-borne bacteria. J Food Process Preserv 2013;37:1022-1030.
26. Abdalla WE, Abdallah EM. Antibacterial activity of ginger (Zingiber Officinale Rosc.) Rhizome: a mini review. Int J Pharmacogn Chinese Med 2018;2:000142.
27. Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 2015;28:603-661.
28. Chambers HF. The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 2001;7:178-182.
29. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123-140.
30. Jafari A, Aslani M, Bouzari S. Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iran J Microbiol 2012;4:102-117.
31. Streeter K, Katouli M. Pseudomonas aeruginosa: A review of their pathogenesis and prevalence in clinical settings and the environment. Infect Epidemiol Microbiol 2016;2:25-32.
32. Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol 2011;19:419-426.
33. Piperaki E-T, Syrogiannopoulos GA, Tzouvelekis LS, Daikos GL. Klebsiella pneumoniae: virulence, biofilm and antimicrobial resistance. Pediatr Infect Dis J 2017;36:1002-1005.
34. Bengoechea JA, Sa Pessoa J. Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS Microbiol Rev 2019;43:123-144.
2. Firmino DF, Cavalcante TTA, Gomes GA, Firmino NCS, Rosa LD, de Carvalho MG, et al. Antibacterial and antibiofilm activities of Cinnamomum sp. essential oil and Cinnamaldehyde: antimicrobial activities. ScientificWorldJournal 2018;2018:7405736.
3. Tongnuanchan P, Benjakul S. Essential oils: extraction, bioactivities, and their uses for food preservation. J Food Sci 2014;79:R1231-1249.
4. Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, Jorge AO. Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. J Appl Oral Sci 2013;21:25-31.
5. Nguefack J, Budde BB, Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol 2004;39:395-400.
6. Osanloo M, Sedaghat MM, Esmaeili F, Amani A. Larvicidal activity of essential oil of Syzygium aromaticum (Clove) in com-parison with its major constituent, eugenol, against Anopheles stephensi. J Arthropod Borne Dis 2018;12:361-369.
7. Husain I, Ahmad R, Chandra A, Raza ST, Shukla Y, Mahdi F. Phytochemical characterization and biological activity evaluation of ethanolic extract of Cinnamomum zeylanicum. J Ethnopharmacol 2018;219:110-116.
8. Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 2008;69: 1732-1738.
9. Brochot A, Guilbot A, Haddioui L, Roques C. Antibacterial, antifungal, and antiviral effects of three essential oil blends. Microbiologyopen 2017;6(4):e00459.
10. Man A, Santacroce L, Jacob R, Mare A, Man L. Antimicrobial activity of six essential oils against a group of human pathogens: A comparative study. Pathogens 2019;8:15.
11. Saeb S, Amin M, Gooybari RS, Aghel N. Evaluation of antibacterial activities of Citrus limon, Citrus reticulata, and Citrus grandis against pathogenic bacteria. Int J Enteric Pathog 2016;4(4): e37103.
12. Lambert R, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 2001;91:453-462.
13. Chaleshtori RS, Rokni N, Razavilar V, Kopaei MR. The evaluation of the antibacterial and antioxidant activity of Tarragon (Artemisia dracunculus L.) essential oil and its chemical composition. Jundishapur J Microbiol 2013;6 (9); e7877.
14. Said-Al Ahl HA, Sarhan AM, Dahab ADMA, Abou-Zeid E-SN, Ali MS, Naguib NY, et al. Essential oils of Anethum graveolens L.: chemical composition and their antimicrobial activities at vegetative, flowering and fruiting stages of development. Int J Plant Sci 2015;1:98-102.
15. Kuglerova M, Tesarova H, Grade JT, Halamova K, Wanyana-Maganyi O, Van Damme P, et al. Antimicrobial and antioxidative effects of Ugandan medicinal barks. Afr J Biotechnol 2011;10:3628-3632.
16. Osanloo M, Abdollahi A, Valizadeh A, Abedinpour N. Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms. Iran J Microbiol 2020;12:43-51.
17. Osanloo M, Sedaghat MM, Sereshti H, Rahmani M, Saeedi Landi F, Amani A. Chitosan nanocapsules of tarragon essential oil with low cytotoxicity and long-lasting activity as a green nano-larvicide. J Nanostruct 2019;9:723-735.
18. Valizadeh A, Shirzad M, Esmaeili F, Amani A.Increased antibacterial activity of Cinnamon Oil Microemulsionin Comparison with Cinnamon Oil Bulk and Nanoemulsion. Nanomed Res J 2018;3:37-43.
19. Raeisi M, Tajik H, Razavi RS, Maham M, Moradi M, Hajimohammadi B, et al. Essential oil of tarragon (Artemisia dracunculus) antibacterial activity on Staphylococcus aureus and Escherichia coli in culture media and Iranian white cheese. Iran J Microbiol 2012;4:30-34.
20. Derakhshan S, Navidinia M, Ahmadi A. Antibacterial activity of Dill (Anethum graveolens) essential oil and antibiofilm activity of Cumin (Cuminum cyminum) alcoholic extract. Infect Epidemiol Microbiol 2017;3:122-126.
21. Ruangamnart A, Buranaphalin S, Temsiririrkkul R, Chuakul W, Pratuangdejkul J. Chemical compositions and antibacterial activity of essential oil from dill fruits (Anethum graveolens L.) cultivated in Thailand. Mahidol Univ J Pharm Sci 2015;42:135-143.
22. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 2006;6:39.
23. Eldahshan OA, Halim AF. Comparison of the composition and antimicrobial activities of the essential oils of green branches and leaves of Egyptian navel orange (Citrus sinensis (L.) Osbeck var. Malesy). Chem Biodivers 2016;13:681-685.
24. Frassinetti S, Caltavuturo L, Cini M, Della Croce C, Maserti B. Antibacterial and antioxidant activity of essential oils from Citrus spp. J Essent Oil Res 2011;23:27-31.
25. Debbarma J, Kishore P, Nayak BB, Kannuchamy N, Gudipati V. Antibacterial activity of ginger, eucalyptus and sweet orange peel essential oils on fish-borne bacteria. J Food Process Preserv 2013;37:1022-1030.
26. Abdalla WE, Abdallah EM. Antibacterial activity of ginger (Zingiber Officinale Rosc.) Rhizome: a mini review. Int J Pharmacogn Chinese Med 2018;2:000142.
27. Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 2015;28:603-661.
28. Chambers HF. The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 2001;7:178-182.
29. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123-140.
30. Jafari A, Aslani M, Bouzari S. Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iran J Microbiol 2012;4:102-117.
31. Streeter K, Katouli M. Pseudomonas aeruginosa: A review of their pathogenesis and prevalence in clinical settings and the environment. Infect Epidemiol Microbiol 2016;2:25-32.
32. Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol 2011;19:419-426.
33. Piperaki E-T, Syrogiannopoulos GA, Tzouvelekis LS, Daikos GL. Klebsiella pneumoniae: virulence, biofilm and antimicrobial resistance. Pediatr Infect Dis J 2017;36:1002-1005.
34. Bengoechea JA, Sa Pessoa J. Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS Microbiol Rev 2019;43:123-144.
| Files | ||
| Issue | Vol 12 No 6 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i6.5032 | |
| Keywords | ||
| Essential oil; Antibacterial activity; Pathogens; Microdilution | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Osanloo M, Ghaznavi G, Abdollahi A. Surveying the chemical composition and antibacterial activity of essential oils from selected medicinal plants against human pathogens. Iran J Microbiol. 2020;12(6):577-583.
