Detection and identification of Chlamydia spp. from pigeons in Iran by nested PCR and sequencing

  • Nadia Golestani Department of Clinical Sciences, School of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
  • Payam Haghighi Khoshkhoo Mail Department of Clinical Sciences, School of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
  • Hossein Hosseini Department of Clinical Sciences, School of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
  • Gita Akbari Azad Department of Clinical Sciences, School of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
Chlamydia psittaci;, Polymerase chain reaction;, Pigeons;, Genotype B;, Chlamydia avium


Background and Objectives: Chlamydia psittaci, an obligate intracellular, Gram-negative zoonotic pathogen, has eight serovars and nine genotypes isolated from avian species with higher frequency in parrots and pigeons. The aim of this study was to characterize Chlamydia spp. using nested PCR and sequencing.
Materials and Methods: A total of 270 pharyngeal swab samples collected randomly from asymptomatic pigeons of 30 pigeon aviaries in Tehran province. DNA was extracted with specific kit and amplified by specific primers in the first PCR and outer membrane protein A (ompA) gene in the second PCR. Positive samples were sequenced and phylogenetic tree analyzed based on the ompA gene.
Results: Records showed that 16 of 30 (53%) pigeon aviaries were positive for Chlamydia spp. Phylogenetic tree analysis revealed that 15 of 16 (93.7%) positive samples, belonged to C. psittaci genotype B whereas the other sample belonged to C. avium. C. psittaci detected in 50% of pigeon aviaries that is high rate in Tehran province.
Conclusion: As C. psittaci is a zoonosis and life threaten pathogen for human being, these results indicate the significance of it detection in asymptomatic pigeons. Also, this is the first report of Chlamydia avium presence in Iranian pigeons which its zoonotic potential is still unknown.


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How to Cite
Golestani N, Haghighi Khoshkhoo P, Hosseini H, Akbari Azad G. Detection and identification of Chlamydia spp. from pigeons in Iran by nested PCR and sequencing. Iran J Microbiol. 12(4):331-337.
Original Article(s)