Anti-proliferative effects of cell wall, cytoplasmic extract of Lactococcus lactis and nisin through down-regulation of cyclin D1 on SW480 colorectal cancer cell line
-
Sareh Sadat Hosseini
,
Hossein Goudarzi
,
Zohreh Ghalavand
,
Bahareh Hajikhani
,
Zahra Rafeieiatani
,
Mojdeh Hakemi-Vala
Abstract
Background and Objectives: Colorectal cancer is one of the most types of cancer. Researchers have shown that lactic acid bacteria have antitumor activity. The cell wall of Lactococcus lactis, as the bacterial cytoplasmic extract and nisin can affect the proliferation of cancer cells. Since cyclin D1 plays an important role in the progression of the cell cycle, its regulation can also be a therapeutic approach. We investigated the antiproliferative effect of cell wall, cytoplasmic extract and nisin on SW480 cancer cell line and the expression level of cyclin D1 gene in treated cancer cells.
Materials and Methods: SW480 cell lines were treated with different concentrations of bacterial cell wall, cytoplasmic extract and nisin. MTT test was also performed. The expression level of cyclin D1 gene was determined using Real time PCR. Data were analyzed using Graph Pad Prism software.
Results: The growth rate of cancer cells treated with nisin has significantly decreased compared to the cancer cells treated by other two substances (p< 0.05). Survival rates of the cancer cells treated by nisin at a concentration of 2000 μg, cytoplasmic extract, and cell wall were 34%, 47% and 49%, respectively. Real-time PCR results showed that cyclin D1 mRNA expression has significantly decreased in nisin treated sw480 cells (P<0.05).
Conclusion: The results of this study show that nisin, bacterial cytoplasmic extract, and bacterial cell wall have antiproliferative effects, which are associated with the decreased expression of cyclin D1 in SW480 cell line.
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20. Zhang X, Sukamporn P, Zhang S, Min KW, Baek SJ. 3,3′-diindolylmethane downregulates cyclin D1 through triggering endoplasmic reticulum stress in colorectal cancer cells. Oncol Rep 2017; 38: 569-574.
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24. Tuo WF, Zhang LW, Yi H, Zhang Y. Short communication: Antiproliferative effect of wild Lactobacillus strains isolated from fermented foods on HT-29 cells. J Dairy Sci 2010; 93: 2362-2366.
25. Tufail M, Hussain S, Malik F. Isolation and evaluation of antibacterial activity of bacteriocin produced by Lactobacillus bulgaricus from yogurt. Afr J Microbiol Res 2011; 5: 3842-3847.
26. Sarika AR, Lipton AP. Isolation of a bacteriocin-producing Lactococcus lactis and application of Its bacteriocin to manage Spoilage bacteria in high-value marine fish under different storage temperatures. Appl Biochem Biotechnol 2012; 167:1280-1289.
27. Ahmadi SH, Ghollasi M, Mahmoodzadeh Hoseini H. The apoptotic impact of nisin as a potent bacteriocin on the colon cancer cells. Microb Pathog 2017; 111:193-197.
28. Li Y, Wei J, Xu C, Zhao Z, You T. Prognostic significance of cyclin D1 expression in colorectal cancer: a meta-analysis of observational studies. PLoS One 2014; 9(4): e94508.
29. Musgrove EA, Caldon CE, Barraclough J, Stone A, Sutherland RL. Cyclin D as a therapeutic target in cancer. Nat Rev Cancer 2011; 11:558-572.
30. Hang L, Fried FB, Guo H, Friedman AD. Cyclin dependent kinase phosphorylation of RUNX1/AML1 on 3 sites increases trans activation potency and stimulates cell proliferation. Blood 2008;111:1193-1200.
31. Maher S, McClean S. Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro. Biochem Pharmacol 2006; 71:1289-1298.
32. Wang S, Han X, Zhang L, Zhang Y, Li H, Jiao Y. Whole peptidoglycan extracts from the Lactobacillus paracasei subsp. Paracasei M5 strain exert anticancer activity in vitro. Biomed Res Int 2018; 2018: 1-11.
2. Uccello M, Malaguarnera G, Basile F, D’agata V, Malaguarnera M, Bertino G, et al. Potential role of probiotics on colorectal cancer prevention. BMC Surg 2012:12 Suppl 1(Suppl 1):S35.
3. Vivarelli S, Falzone L, Sofia M, Daria B, Carlo N, Massimo G, et al. Benefits of using probiotics as adjuvants in anticancer therapy (Review). World Acad Sci J 2019:1;125-135.
4. Biemar F, Foti M. Global progress against cancer challenges and opportunities. Cancer Biol Med 2013; 10: 183-186.
5. Górska A, Przystupski D, Niemczura M, Kulbacka J. Probiotic bacteria: a promising tool in cancer prevention and therapy. Curr Microbiol 2019; 76: 939-949.
6. Ai-Lian Song A, Lionel LA, Swee Hua L, Abdul Rahim R. A review on Lactococcus lactis: from food to factory. Microb Cell Fact 2017; 16:55.
7. Nam EJ, Ritchie R, Kamarajan P, Miao D, Kapila Y. Nisin, an apoptogenic bacteriocin and food preservative, attenuates HNSCC tumorigenesis via CHAC1. Cancer Med 2012; 1: 295-305.
8. Ren D, Jianwei Z, Shengjie G, Liu H, Yu H. Antimicrobial characteristics of Lactic acid bacteria isolated from homemade fermented food. Biomed Res Int 2018; 2018:5416725.
9. Hosseini SS, Hajikhani B, Faghihloo E, Goudarzi H. Increased expression of caspase genes in colorectal cancer cell line by nisin. Arch Clin Infect Dis 2020; 15 (2); e97734.
10. Kohwi Y, Konishi K. Antitumor effect of normal intestinalmicroflora on Ehrlich ascites tumor. Jpn J Cancer Res 1988; 79: 109 -116.
11. Sekine K, Toida T, Saito M, Kuboyama M, Kawashima T, Hashimoto Y. A new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Cancer Res 1985; 45:1300-1307.
12. Ji Yeon K, Hee Jong W, Young K, Kyoung K, Hyong L. Cell cycle dysregulation induced by cytoplasm of Lactococcus lactis ssp. lactis in SNUC2A, a colon cancer cell line. Nutr Cancer 2003; 46:197-201.
13. He Y, Franco OE, Jiang M, Williams K, Love HD, Coleman I, et al. Tissue-specific consequences of cyclin D1 overexpression in prostate cancer progression. Cancer Res 2007; 67:8188-8197.
14. Mylona E, Tzelepis K, Theohari I, Giannopoulou I, Papadimitriou C, Nakopoulou L. Cyclin D1 in invasive breast carcinoma: favourable prognostic significance in unselected patients and within subgroups with an aggressive phenotype. Histopathology 2013; 62:472-480.
15. Chen WC, Lin MS, Zhang BF, Fang J, Zhou Q, Hu Y, et al. Survey of molecular profiling during human colon cancer development and progression by immunohistochemical staining on tissue microarray. World J
Gastroenterol 2007; 13: 699-708.
16. Bali A, O’Brien PM, Edwards LS, Sutherland RL, Hacker NF, Henshall SM. Cyclin D1, p53, and p21Waf1/Cip1 expression is predictive of poor clinical outcome in serous epithelial ovarian cancer. Clin Cancer Res 2004;10:5168-5177.
17. Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, et al. Colorectal cancer. Lancet 2010; 375:1030-1047.
18. VanArsdale T, Boshoff C, Arndt KT, Abraham RT. Molecular pathways: targeting the cyclin D-CDK4/6 axis for cancer treatment. Clin Cancer Res 2015; 21: 2905-2910.
19. Kim SY, Lee KW, Kim JY, Lee HJ. Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells. Biofactors 2004; 22: 119-122.
20. Zhang X, Sukamporn P, Zhang S, Min KW, Baek SJ. 3,3′-diindolylmethane downregulates cyclin D1 through triggering endoplasmic reticulum stress in colorectal cancer cells. Oncol Rep 2017; 38: 569-574.
21. Amrouche T, Boutin Y, Prioult G, Fliss I. Effects of bifidobacterial cytoplasm, cell wall and exopolysaccharide on mouse lymphocyte proliferation and cytokine production. Int Dairy J 2006; 16: 70-80.
22. Han KJ, Lee NK, Park H, Paik HD. Anticancer and anti-inflammatory activity of probiotic Lactococcus lactis NK34. J Microbiol Biotechnol 2015;25:1697-1701.
23. Thirabunyanon M, Boonprasom P, Niamsup P. Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. Biotechnol Lett 2008; 31: 571-576.
24. Tuo WF, Zhang LW, Yi H, Zhang Y. Short communication: Antiproliferative effect of wild Lactobacillus strains isolated from fermented foods on HT-29 cells. J Dairy Sci 2010; 93: 2362-2366.
25. Tufail M, Hussain S, Malik F. Isolation and evaluation of antibacterial activity of bacteriocin produced by Lactobacillus bulgaricus from yogurt. Afr J Microbiol Res 2011; 5: 3842-3847.
26. Sarika AR, Lipton AP. Isolation of a bacteriocin-producing Lactococcus lactis and application of Its bacteriocin to manage Spoilage bacteria in high-value marine fish under different storage temperatures. Appl Biochem Biotechnol 2012; 167:1280-1289.
27. Ahmadi SH, Ghollasi M, Mahmoodzadeh Hoseini H. The apoptotic impact of nisin as a potent bacteriocin on the colon cancer cells. Microb Pathog 2017; 111:193-197.
28. Li Y, Wei J, Xu C, Zhao Z, You T. Prognostic significance of cyclin D1 expression in colorectal cancer: a meta-analysis of observational studies. PLoS One 2014; 9(4): e94508.
29. Musgrove EA, Caldon CE, Barraclough J, Stone A, Sutherland RL. Cyclin D as a therapeutic target in cancer. Nat Rev Cancer 2011; 11:558-572.
30. Hang L, Fried FB, Guo H, Friedman AD. Cyclin dependent kinase phosphorylation of RUNX1/AML1 on 3 sites increases trans activation potency and stimulates cell proliferation. Blood 2008;111:1193-1200.
31. Maher S, McClean S. Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro. Biochem Pharmacol 2006; 71:1289-1298.
32. Wang S, Han X, Zhang L, Zhang Y, Li H, Jiao Y. Whole peptidoglycan extracts from the Lactobacillus paracasei subsp. Paracasei M5 strain exert anticancer activity in vitro. Biomed Res Int 2018; 2018: 1-11.
| Files | ||
| Issue | Vol 12 No 5 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i5.4603 | |
| Keywords | ||
| Cell wall; Cytoplasmic extract; Nisin; Cyclic redundancy check; Cyclin D1 | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Hosseini SS, Goudarzi H, Ghalavand Z, Hajikhani B, Rafeieiatani Z, Hakemi-Vala M. Anti-proliferative effects of cell wall, cytoplasmic extract of Lactococcus lactis and nisin through down-regulation of cyclin D1 on SW480 colorectal cancer cell line. Iran J Microbiol. 2020;12(5):424-430.
