The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap
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Abstract
Background and Objectives: Lontar (Borassus flabellifer L.) is widely grown in Indonesia and one of its products is palm sap. Palm sap contains a high level of sugar, making it suitable as a medium to increase the lactic acid bacteria (LAB) production of exopolysaccharides (EPS). This study aimed to isolate the EPS-producing LAB from palm sap and evaluate its EPS production. LAB isolation was carried out on MRS agar containing 0.5% CaCO3.
Materials and Methods: The screening and production of EPS were carried out on MRS media supplemented with 10% sucrose. The molecular identification of the selected EPS-producing LAB was based on 16S rDNA. A quantitative analysis of EPS polymer dry mass and total sugar was conducted using one-way ANOVA.
Results: In this study, five EPS-producing LABs were found: Fructobacillus fructosus N4, Leuconostoc mesenteroides N5, Leuconostoc mesenteroides N7, Leuconostoc mesenteroides N9, and Fructobacillus fructosus N10. The highest EPS yield in liquid media was 10.997 ± 1.591 g/L by Leuconostoc mesenteroides N7, whereas the lowest was 4.505 ± 0.459 g/L by Fructobacillus fructosus N10.
Conclusion: This study found Fructobacillus fructosus strains as EPS producers that have never been reported before.
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15. Shao LZ, Wu H, Zhang W, Chen L, Ai B, Guo B. Partial characterization and immunostimulatory activity of exopolysac-charides from Lactobacillus rhamnosus KF5. Carbohydr Polym 2014; 107: 51-56.
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18. Alegría A, Delgado S, Florez A, Mayo B. Identification, typing, and functional characterization of Leuconostoc spp. strains from traditional, starter-freecheeses. Dairy Sci Technol 2013;93: 657-673.
19. Endo A, Tanizawa Y, Tanaka N, Maeno S, Kumar H, Shiwa Y, et al. Comparative genomics of Fructobacillus spp. And Leuconostoc spp. reveals nichespecific evolution of Fructobacillus spp. BMC Genomics 2015; 16: 1117.
20. Han J, Hang F, Guo B, Liu Z, You C, Wu Z. Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose. Journal of Carbohydr Polym 2014; 112:556-562.
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22. Feng F, Zhou Q, Yang Y, Zhao F, Du R, Han Y, et al. Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product. Int J Biol Macromol 2018; 113: 45-50.
23. Wu Z, Wang G, Pan D, Guo Y, Zeng X, Sun Y, et al. Inflamation-related pro-apoptotic activity of exopolysaccharides isolated from Lactococcus lactis subsp. lactis. Benef Microbes 2016; 7: 761-768.
24. Vettori MHPG, Franchetti SMM, Contiero J. Structural characterization of a new dextran with a low degree of branching produce by Leuconostoc mesenteroides FT045B dextransucrase. Carbohydr Polym 2012;88: 1440-1444.
2. Laino J, Villena J, Kanmani P, Kitazawa H. Immunoregulatory effect triggered by lactic acid bacteria exopolysaccharides: New insight into Molecular Interactions with host cells. Microorganisms 2016;4:27.
3. Hijum SAFTV, Geel-Schutten GHV, Rahaoui H, Maarel MJECVD, Dijkhuizen L. Characterization of a novel fructosyltransferase from Lactobacillus reuteri that synthesizes high-molecular-weight inulin and inulin oligosaccharides. Appl Environ Microbiol 2002; 68: 4390-4398.
4. Vu B, Chen M, Crawford RJ, Ivanova EP. Bacterial extracellular polysaccharides involved in biofilm formation. Molecules 2009; 14: 2535-2554.
5. Torino MI, de Valdez GF, Mozzi F. Biopolymers from lactic acid bacteria. Novel application sin foods and beverages. Front Microbiol 2015; 6: 834.
6. Patil P, Wadehra A, Munjal K, Behare P. Isolation of exopolysaccharides producing lactic acid bacteria from dairy products. Asian J Dairy Food Res 2015;34: 280-284.
7. Bajpai VK, Rather IA, Majumder R, Shukla S, Aeron A, Kim K, et al. Exopolysaccharide and lactic acid bacteria: Perception, functionality and prospects. Bangladesh J Pharmacol 2015; 11: 1.
8. Chen Y, Zhang H, Wang YX, Nie SP, Li C, Xie MY. Acetylation and carboxymethylation of the polysaccharide from Ganoderma atrum and their antioxidant and immunomodulating activities. Food Chem 2014; 156:279-288.
9. Li S, Huang R, Shah NP, Tao X, Xiong Y, Wei H. Antioxidant and antibacterial activities of exopolysaccharides from Bifido bacterium bifidum WBIN03 and Lactobacillus plantarum R315. J Dairy Sci 2014;97: 7334-7343.
10. Wang J, Xiao Z, Zheng T, Congcong H, Yawei Y, Zhennai Y. Isolation and characterization of exopolysaccharide-producing Lactobacillus plantarum SKT109 from Tibet Kefir. Pol J Food Nutr Sci 2015; 65:269-279.
11. Sirajuddin S, Mulyadi M, Dirawan G, Amir F, Pertiwi N. Conservation status of lontar palm trees (Borassus flabellifer Linn) in Jeneponto district, south Sulawesi, Indonesia. J Trop Crop Sci 2016; 3:28-33.
12. Lule V, Singh R, Behare P, Tomar SK. Comparison of exopolysaccharide production by indigenous Leuconostoc mesenteroides strains in whey medium. Asian J Dairy Food Res 2015;34: 8-12.
13. Tayo BA, Ishola R, Oyewunmi T. Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confuse strains. Biotechnol Rep 2018;19:e00271.
14. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorymetric method for determination of sugar and related substances. Anal Chem 1956; 28: 350-356.
15. Shao LZ, Wu H, Zhang W, Chen L, Ai B, Guo B. Partial characterization and immunostimulatory activity of exopolysac-charides from Lactobacillus rhamnosus KF5. Carbohydr Polym 2014; 107: 51-56.
16. Siddiqui NN, Afsheen A, Alba S, Shah AUQ, Antonio M. Structural analysis and characterization of dextran produced by wild and mutant strains of Leuconostoc mesenteroides. Carbohydr Polym 2014; 99: 331-338.
17. Joo Seo B, Bajpai VK, Rather IA, Park YH. Partially purified exopolysaccharide from Lactobacillus plantarum YML009 with total Phenolic content, antioxidant, and free radical scavenging efficacy. Indian J Pharm Educ Res 2015; 49: 282-292.
18. Alegría A, Delgado S, Florez A, Mayo B. Identification, typing, and functional characterization of Leuconostoc spp. strains from traditional, starter-freecheeses. Dairy Sci Technol 2013;93: 657-673.
19. Endo A, Tanizawa Y, Tanaka N, Maeno S, Kumar H, Shiwa Y, et al. Comparative genomics of Fructobacillus spp. And Leuconostoc spp. reveals nichespecific evolution of Fructobacillus spp. BMC Genomics 2015; 16: 1117.
20. Han J, Hang F, Guo B, Liu Z, You C, Wu Z. Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose. Journal of Carbohydr Polym 2014; 112:556-562.
21. Joshi SR, Koijam K. Exopolysaccharide production by a lactic acid bacteria, Leuconostoc lactis isolated from ethnically fermented beverage. Natl Acad Sci Lett 2014; 37: 59-64.
22. Feng F, Zhou Q, Yang Y, Zhao F, Du R, Han Y, et al. Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product. Int J Biol Macromol 2018; 113: 45-50.
23. Wu Z, Wang G, Pan D, Guo Y, Zeng X, Sun Y, et al. Inflamation-related pro-apoptotic activity of exopolysaccharides isolated from Lactococcus lactis subsp. lactis. Benef Microbes 2016; 7: 761-768.
24. Vettori MHPG, Franchetti SMM, Contiero J. Structural characterization of a new dextran with a low degree of branching produce by Leuconostoc mesenteroides FT045B dextransucrase. Carbohydr Polym 2012;88: 1440-1444.
| Files | ||
| Issue | Vol 12 No 5 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i5.4605 | |
| Keywords | ||
| Borassus flabellifer; Exopolysaccharide; Fructobacillus fructosus; Lactic acid bacteria; Leuconostoc mesenteroides | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Ma’unatin A, Harijono H, Zubaidah E, Rifa’i M. The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap. Iran J Microbiol. 2020;12(5):437-444.
