The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap

  • Anik Ma'unatin Mail Department of Agricultural Technology, Brawijaya University, Malang, East Java, Indonesia AND Department of Chemistry, Maulana Malik Ibrahim State Islamic University Malang, Malang, East Java, Indonesia
  • Harijono Harijono Department of Agricultural Technology, Brawijaya University, Malang, East Java, Indonesia
  • Elok Zubaidah Department of Agricultural Technology, Brawijaya University, Malang, East Java, Indonesia
  • Muhaimin Rifa'i Department of Biology, Brawijaya University, Malang, East Java, Indonesia
Keywords:
Borassus flabellifer;, Exopolysaccharide;, Fructobacillus fructosus;, Lactic acid bacteria;, Leuconostoc mesenteroides

Abstract

Background and Objectives: Lontar (Borassus flabellifer L.) is widely grown in Indonesia and one of its products is palm sap. Palm sap contains a high level of sugar, making it suitable as a medium to increase the lactic acid bacteria (LAB) production of exopolysaccharides (EPS). This study aimed to isolate the EPS-producing LAB from palm sap and evaluate its EPS production. LAB isolation was carried out on MRS agar containing 0.5% CaCO3.
Materials and Methods: The screening and production of EPS were carried out on MRS media supplemented with 10% sucrose. The molecular identification of the selected EPS-producing LAB was based on 16S rDNA. A quantitative analysis of EPS polymer dry mass and total sugar was conducted using one-way ANOVA.
Results: In this study, five EPS-producing LABs were found: Fructobacillus fructosus N4, Leuconostoc mesenteroides N5, Leuconostoc mesenteroides N7, Leuconostoc mesenteroides N9, and Fructobacillus fructosus N10. The highest EPS yield in liquid media was 10.997 ± 1.591 g/L by Leuconostoc mesenteroides N7, whereas the lowest was 4.505 ± 0.459 g/L by Fructobacillus fructosus N10.
Conclusion: This study found Fructobacillus fructosus strains as EPS producers that have never been reported before.

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Published
2020-10-31
How to Cite
1.
Ma’unatin A, Harijono H, Zubaidah E, Rifa’i M. The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap. Iran J Microbiol. 12(5):437-444.
Section
Original Article(s)